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Plasmid#134132PurposeBacterial expression of His-TEV-Ska1 and Ska2DepositorInsertHis-TEV-human Ska1 ; human Ska2
UseTagsHis-TEVExpressionBacterialMutationK183A K184APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2-hFAM 3B-His
Plasmid#172192PurposeCoding sequence of human FAM3B pCS2 C terminal 6xHis tag used for protein purification from conditioned mediumDepositorInserthFAM 3B
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
ZJOM108
Plasmid#133665PurposeIntegration of mTagBFP2-Tlg1 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.DepositorInsertp1k-mTagBFP2-TLG1 (TLG1 Budding Yeast)
UseTagsmTagBFP2ExpressionYeastMutationPromoterAvailable sinceApril 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
MS2_adRNA-MCP_ADAR2 DD_NLS
Plasmid#124704PurposeExpresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD_NLSDepositorInsertMCP_ADAR2 DD_NLS
UseAAVTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
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y2
Plasmid#53586PurposeProduces an active Arabidopsis thaliana lycopene epsilon-cyclase in E. coli. Use with pAC-LYC or pAC-LYCipi to make delta-carotene in E. coli.DepositorInsertlcyE (LUT2 Mustard Weed)
UseTagsExpressionMutationPromoterAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_+1D
Plasmid#149389PurposeP. aeruginosa PA14 CRISPR2 locus, with 1bp addition downstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutation1bp inserted downstream of the IHFprox sitePromoterpBADAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry-miniSOG-Cx43-7
Plasmid#57787PurposeLocalization: Gap Junctions, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertCx43
UseTagsPA-mCherry-miniSOGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMig (Martins Lab)
Plasmid#14125DepositorInsertIRES-EGFP
UseRNAi and RetroviralTagsExpressionMammalianMutationCla I sites were silenced in the original pRetroS…PromoterAvailable sinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_10A
Plasmid#91207PurposeDirect Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: noneDepositorInsert35S:AtCas9 + AtU6:gRNA
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_RANBP2_Ran-BP2
Plasmid#109839PurposeProtein expression and purification of RANBP2_Ran-BP2DepositorInsertRANBP2_Ran-BP2 (RANBP2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1810
Plasmid#91040PurposeModule A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertCsy4-P2A-TaCas9_dead (D10A + H840A)
UseCRISPRTagsExpressionMutationD10A, H840APromoterZmUbiAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
mouse Cavin-1
Plasmid#50441Purposeexpresses mouse Cavin-1 in mammalian cellsDepositorInsertCavin-1 (Cavin1 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-SIK1
Plasmid#51577PurposeConstitutively express nucleolus localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsSIK1 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-SNF7
Plasmid#51574PurposeConstitutively express endosome localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsSNF7 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJL2
Plasmid#184130PurposeFor rescuing null mrp-1 mutation and labelling mrp-1 protein. Translational fusion construct. pPD95.75_Pmrp-1::mrp-1(isoform C cDNA)::gfp.DepositorInsertMRP-1 (mrp-1 Nematode)
UseTagsGFPExpressionWormMutationPromoterPmrp-1Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL1
Plasmid#184129PurposeFor rescuing null mrp-1 mutation. pPD95.77_Pmrp-1::mrp-1(isoform C cDNA)::SL2::gfp. SL2 prevents fusion of MRP-1 and GFP.DepositorInsertMRP-1 (mrp-1 Nematode)
UseTagsGFPExpressionWormMutationPromoterPmrp-1 (3kb)Available sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6_T7_INT[6xminimal_Puf1]
Plasmid#107608PurposeTransient expression of an "INT" construct [bearing six minimal Puf1 stem-loops; 'INT' design: Shechner et al., Nature Methods 2015], in mammalian cells, U6 promoterDepositorInsert6x minimal Puf1 motif
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1710
Plasmid#91039PurposeModule A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_H840A nickase, Terminator: HSPDepositorInsertCsy4-P2A-TaCas9_H840A nickase
UseCRISPRTagsExpressionMutationH840APromoterZmUbiAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC364
Plasmid#91213Purposeprotoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, Csy4 processing)DepositorInsert2 gRNAs targeting tomato ARF8A
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only