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Plasmid#73920PurposeGST fusion- Erp1 cytoplasmic tailDepositorInsertErp1 CT
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
P415 Ret2LD
Plasmid#73875PurposeRet2LD - His tagged (2α helices+μHD deleted)DepositorInsertRet2LD
UseTagsHisExpressionYeastMutationPromoterMet25Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
P415 Ret2LD2α
Plasmid#73873PurposeRet2LD2α - His tagged (μHD deleted)DepositorInsertRet2LD2α
UseTagsHisExpressionYeastMutationPromoterMet25Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
p415 Ret2 I155A
Plasmid#73888PurposeI155A mutationDepositorInsertRet2 I155A
UseTagsExpressionYeastMutationIle 155 AlaPromoterMet25Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
p415 Ret2 I149A
Plasmid#73887PurposeI149A mutationDepositorInsertRet2 I149A
UseTagsExpressionYeastMutationIle 149 AlaPromoterMet25Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
p415 Ret2
Plasmid#73872PurposeRet2 (full length)DepositorInsertRet2
UseTagsExpressionYeastMutationPromoterMet25Available sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSL3 - Brr2 del113 N1104L
Plasmid#90253Purposeprotein expressionDepositorInsertBRR2
UseTagsTAPExpressionYeastMutationdeleted first 113 amino acids and amino acid N110…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSL11 - Brr2 del422 N1104L
Plasmid#90261Purposeprotein expressionDepositorInsertBRR2
UseTagsTAPExpressionYeastMutationdeleted first 422 amino acids and amino acid N110…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSL12 - Brr2 del422 Q904E
Plasmid#90262Purposeprotein expressionDepositorInsertBRR2
UseTagsTAPExpressionYeastMutationdeleted first 422 amino acids and amino acid Q904EPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSL13 - Brr2 del422 R1107L
Plasmid#90263Purposeprotein expressionDepositorInsertBRR2
UseTagsTAPExpressionYeastMutationdeleted first 422 amino acids and amino acid R110…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pGI3EM20C
Plasmid#135487PurposeAgrobacterium T-DNA with divergent Spun H2A/Bpr driving H2B-tdTomato and hphDepositorInserthph SpunH2A/Bpr H2B-tdTomato
UseTagstdTomatoExpressionBacterial and YeastMutationPromoterSpunH2A/Bpr (divergent)Available sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM22C
Plasmid#135488PurposeAgrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hphDepositorInserthph SpunH2A/Bpr LifeAct-tdTomato
UseTagsLifeActExpressionBacterial and YeastMutationPromoterSpunH2A/Bpr (divergent)Available sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAV1-mEGFP
Plasmid#27704DepositorInsertcaveolin-1 (CAV1 Canis lupus familiaris)
UseTagsmonomeric EGFPExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBES1-LacOx50-Hyg
Plasmid#210261PurposeIntegrates 50xLacO repeats upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.DepositorInsert50xLacO repeats derived from: PMID: 12864855.
UseTagsExpressionMutationPromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Plas-gRNA-Z3EV
Plasmid#157659PurposeGFP gene and gRNA under Z3EV expression control (estradiol sensor), with Z3EV transcription factor to insert in the genome.DepositorInsertGFP-gRNA NatMX Z3EV
UseInsert storage (replicative in e. coli)TagsExpressionMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMito-dCherry-FRB
Plasmid#186573PurposeExpression of "dark MitoTrap" - an FRB-domain targeted to the mitochondria that is the same size as pMito-mCherry-FRB but is non-fluorescent - in human cellsDepositorInsertDark MitoTrap
UseTagsExpressionMammalianMutationMutation of "K70N" to render mCherry no…PromoterCMVAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Plas-gRNA-LEU2
Plasmid#157656PurposeGFP and gRNA tag for protein and mRNA quantification, to attache to the 3' end of the gene coding sequenceDepositorInsertGFP-gRNA
UseInsert storage (replicative in e. coli)TagsGFP-gRNAExpressionMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Plas-CRISPR_reporter
Plasmid#157658PurposeCRISPR reporter for mRNA quantification, integrative plasmid into NPR2 geneDepositorInsertdCAS9-VP64, mCherry, KanMX
UseInsert storage (replicative in e. coli)TagsExpressionMutationN28D in mCherry- please see depositor comments be…PromoterAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
B52_puro_empty_gRNA
Plasmid#197557Purposesites for cloning two gRNA with puromycin cassetteDepositorTypeEmpty backboneUseEmpty grna backbone with puromycin resistance geneTagsExpressionMammalianMutationPromoterU6 for gRNA, CMV for puromycin resistance.Available sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEM_Δflv3.sm
Plasmid#185533Purposea pGEM-T easy vector containing the streptomycin/spectinomycin resistance cassette flanked by the two regions for the double homologous recombination on the flv3 locusDepositorInsertaadA gene flanked by flv3 upstream and downstream regions
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only