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Plasmid#172148PurposeAccessory Plasmid for PACE of pylBCD variantsDepositorInsertPpsp gIII.1TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT
UseTagsExpressionBacterialMutationgIII(P29*); [strong promoter eG, strong RBS SD8] …PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD/His-D-iLight
Plasmid#170270PurposeExpression of iLight with 6xHis N-terminal tag in bacteria.DepositorInsertiLight
UseTags6xHis tagExpressionBacterialMutationSee CommentsPromoteraraBADAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2C Δ5H-H1
Plasmid#163584Purposeexpress Dcp2 FL-H1 at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationTruncation of aa1-326; the first HLM (EDQLKSYAEEQ…PromoterDCP2 promoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 300 AAAA
Plasmid#163579Purposeexpress Dcp2 300 AAAA at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationTruncation of C-terminal domain (301-970); mutati…PromoterDCP2 promoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 300 ΔH1
Plasmid#163578Purposeexpress Dcp2 300 ΔH1 at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationTruncation of C-terminal domain (301-970); Lysine…PromoterDCP2 promoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
px330 WAPL gRNA
Plasmid#165595PurposeInsertion of WAPL degromDepositorInsertWAPL (Wapl Mouse)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
px330 BRM gRNA
Plasmid#165586PurposeInsertion of BRM degronDepositorInsertBRM (Smarca4 Mouse)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFlox_N-BRM_BSD_FKBPF36V_EYFPF36V
Plasmid#163876PurposeAcute Depletion of BRM-EYFPDepositorInsertBrm (Smarca2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM457 EF1alpha EBFP2-P2A-BlastR (TUPV8)
Plasmid#139237PurposemMoClo TUPV8, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCSDepositorInsertEBFP2-P2A-Blasticidin S Deaminase
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM456 EF1alpha EBFP2-P2A-BlastR (TUPV7)
Plasmid#139236PurposemMoClo TUPV7, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCSDepositorInsertEBFP2-P2A-Blasticidin S Deaminase
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM454 EF1alpha EBFP2-P2A-BlastR (TUPV5)
Plasmid#139234PurposemMoClo TUPV5, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCSDepositorInsertEBFP2-P2A-Blasticidin S Deaminase
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM453 EF1alpha EBFP2-P2A-BlastR (TUPV4)
Plasmid#139233PurposemMoClo TUPV4, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCSDepositorInsertEBFP2-P2A-Blasticidin S Deaminase
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM452 EF1alpha EBFP2-P2A-BlastR (TUPV3)
Plasmid#139232PurposemMoClo TUPV3, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCSDepositorInsertEBFP2-P2A-Blasticidin S Deaminase
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM451 EF1alpha EBFP2-P2A-BlastR (TUPV2)
Plasmid#139231PurposemMoClo TUPV2, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCSDepositorInsertEBFP2-P2A-Blasticidin S Deaminase
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPIC_pet28a
Plasmid#131654PurposeBacterial expression of ETS gene SPICDepositorInsertSPIC (SPIC Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-His-ELP(MV7E2)3-ddFLN4-SpyCatcher
Plasmid#157674PurposeExpression of SpyCatcher with ddFLN4, ELP linker, His6x tag, and ybbR Tag.DepositorInsertSpyCatcher with ddFLN4, ELP, Histag and ybbR tag
UseTagsddFLN4, ELP, Histag and ybbR tagExpressionBacterialMutationPromoterT7Available sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE80L-His-SpyTag-XMod(XL2V)-Doc
Plasmid#157676PurposeExpression of XMod-Doc mutant XL2V (All LEUs were mutated to VAL in XMod region) with His6x tag and SpyTag.DepositorInsertXMod-Doc mutant XL2V with Histag and SpyTag
UseTagsHistag and SpyTagExpressionBacterialMutationPromoterT5Available sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD150 ZF43x12-Spaced-Far_EYFP
Plasmid#138895PurposeEYFP reporter vector for the ZF43x12-Spaced-Far promoterDepositorInsertZF43x12-Spaced-Far
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x12-Spaced-FarAvailable sinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD147 ZF43x8-Spaced-Far_EYFP
Plasmid#138893PurposeEYFP reporter vector for the ZF43x8-Spaced-Far promoterDepositorInsertZF43x8-Spaced-Far
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x8-Spaced-FarAvailable sinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD145 ZF43x4-Spaced-Far_EYFP
Plasmid#138891PurposeEYFP reporter vector for the ZF43x4-Spaced-Far promoterDepositorInsertZF43x4-Spaced-Far
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x4-Spaced-FarAvailable sinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only