Addgene

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pcDNA3.1/GS-DJ1-D149A-V5

Plasmid

#29345

Depositor

Mark Cookson

Article

Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

Insert

DJ1 (PARK7 Human)

Use

Tags

His6 and V5 epitope

Expression

Mammalian

Mutation

Asp149Ala

Promoter

Available since

April 20, 2011

Availability

Academic Institutions and Nonprofits only
pBW2066_pCAG-PV1-NTD-ENTRY-L1-GID1-NLS-BGHpA

Plasmid

#108844

Purpose

Insert protein fragment into MluI/BspEI to fuse to GID1/NLS tags on C-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

GID1,NLS

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pSicoR (Puro) NEMO shRNA1

Plasmid

#22505

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

NEMO shRNA1 (Ikbkg Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pMyc-CMV1-huTLR4mut-C2141A

Plasmid

#53527

Purpose

expression of mutant TLR4 in mammalian cells

Depositor

Linda Yu

Article

Kuo et al Cell Death Differ. 2015 Jan 30. doi: 10.

Insert

TLR4 C2141A (TLR4 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

C2141A

Promoter

CMV

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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pSK+ chickBMPR-1B DN

Plasmid

#49529

Purpose

cloning vector for dominant negative chick BMP receptor 1B

Depositor

Peter ten Dijke, Lee Niswander

Article

Zou et al Genes Dev. 1997 Sep 1;11(17):2191-203.

Insert

BMPR-1B DN (BMPR1B Chicken)

Use

Tags

Expression

Bacterial

Mutation

dominant negative mutation (K231R); also P2A, M11…

Promoter

T3

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only
JPF0419v

Plasmid

#124021

Purpose

Encodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pCAG-Lyn-iRFP713_pCAG-NES-mAzamiGreen_pCAG-p38KTR-mCerulean_pCAG-H2B::mScarlet

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCDB267

Plasmid

#80695

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gHHH_06

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gHHH_06

Use

Tags

OsmY-10His-Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCDB278

Plasmid

#80694

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gHH_44

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gHH_44

Use

Tags

OsmY-10His-Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCDB294

Plasmid

#80693

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gHEEE_02

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gHEEE_02

Use

Tags

OsmY-10His-Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCDB199

Plasmid

#80691

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEHE_06

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gEHE_06

Use

Tags

OsmY-10His-Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCDB289

Plasmid

#80690

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEEHE_02

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gEEHE_02

Use

Tags

OsmY-10His-Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDB185

Plasmid

#80688

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEEH_04

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gEEH_04

Use

Tags

OsmY-10His-Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDB285

Plasmid

#80687

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEEEH_04

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gEEEH_04

Use

Tags

OsmY-10His-Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-27b-Decoy.hPGK.GFP.WPRE

Plasmid

#46603

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-27b Decoy (MIR27B, Mir27b Mouse, Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-HA CASTOR2 ACT2

Plasmid

#84494

Purpose

overexpression of CASTOR2 ACT2 domain (aa 171-end)

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR2 ACT2 (GATSL2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

171-last amino acid

Promoter

CMV

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-Flag CASTOR2 ACT1

Plasmid

#84493

Purpose

overexpression of CASTOR2 ACT1 domain (aa 1-171)

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR2 ACT1 (GATSL2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

1-171 amino acid

Promoter

CMV

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-HA CASTOR1 ACT2

Plasmid

#84492

Purpose

overexpression of CASTOR1 ACT2 domain (aa 169-end)

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 ACT2 (GATSL3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

169-last amino acid

Promoter

CMV

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-Flag CASTOR1 ACT1

Plasmid

#84491

Purpose

overexpression of CASTOR1 ACT1 domain (aa 1-169)

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 ACT1 (GATSL3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

1-169 amino acid

Promoter

CMV

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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MigR1-Myc-Nterm TRIB2

Plasmid

#131153

Purpose

Retroviral expression of N terminus of mouse TRIB2

Depositor

Warren Pear, Karen Keeshan

Article

Keeshan et al Cancer Cell. 2006 Nov;10(5):401-11. doi:

Insert

Trib2 (Trib2 Mouse)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

contains amino acids 1-145

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pUASP YFP Rab32 WT

Plasmid

#37738

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab32 (Rab32 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Promoter

GAL4

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only