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Showing: 6481 - 6500 of 18164 results

  4. FKBP-EGFP-RanGAP*

    Plasmid
    #175238
    Purpose
    Bacterial expression and purification, low affinity SUMOylation substrate that can recruited to FRB with rapamycin, point mutation F562A reduces affinity for E2, increasing the Km
    Depositor
    Michael Rosen
    Article
    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d
    Insert
    RANGAP1 (RANGAP1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Amino acid 562 F to A
    Promoter
    tac
    Available since
    Oct. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    175238-plasmid-map-sequence-id-345469

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  6. pJH859

    Plasmid
    #172177
    Purpose
    PylB overexpression and purification
    Depositor
    Matthew Bennett
    Article
    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:
    Insert
    PT7/Lac-[His6-TEV-SUMO-PylB.JM10.1deopt]
    Use
    Tags
    Expression
    Bacterial
    Mutation
    pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…
    Promoter
    Available since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172177-plasmid-map-sequence-id-338578

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  12. pJH191

    Plasmid
    #172149
    Purpose
    Accessory Plasmid for PACE of pylBCD variants
    Depositor
    Matthew Bennett
    Article
    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:
    Insert
    Ppsp gIII.2TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    gIII(P29*, Y184*); [strong promoter eG, strong RB…
    Promoter
    Available since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172149-plasmid-map-sequence-id-341959

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  13. pJH61

    Plasmid
    #172143
    Purpose
    Accessory Plasmid for PACE of pylBCD variants
    Depositor
    Matthew Bennett
    Article
    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:
    Insert
    Ppsp gIII.1TAG, luxAB; Plpp [eG SD8] pylS; PproK pylT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    gIII(P29*); [strong promoter eG, strong RBS SD8] …
    Promoter
    Available since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172143-plasmid-map-sequence-id-338567

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  14. pJH190

    Plasmid
    #172148
    Purpose
    Accessory Plasmid for PACE of pylBCD variants
    Depositor
    Matthew Bennett
    Article
    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:
    Insert
    Ppsp gIII.1TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    gIII(P29*); [strong promoter eG, strong RBS SD8] …
    Promoter
    Available since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172148-plasmid-map-sequence-id-338790

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Showing: 6481 - 6500 of 18164 results