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Plasmid#68705PurposeFrancisella tularensis subsp. novicida Cas9DepositorInsertFtCas9
UseCRISPRTagsHA-NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-PEmax-P2A-GFP
Plasmid#202000PurposeBased on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.DepositorInsertPEmax-P2A-GFP
UseCRISPR and Synthetic BiologyTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-TET1-Hygro
Plasmid#104404Purposetransient expression of dCas9-TET1 fusion proteinDepositorInsertTET1 (TET1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
1782_pAAV-U6-Ai9-Sa-gRNA2-CB-SACas9-HA-OLLAS-spA_C
Plasmid#135978PurposegRNA against the right loxP site of the Ai9 alleleDepositorInsertAi9 gRNA2
UseAAV, CRISPR, and Mouse TargetingTagsHAExpressionMutationPromoterCBAvailable sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
1781_pAAV-U6-Ai9-Sa-gRNA1-CB-SACas9-HA-OLLAS-spA_C
Plasmid#135979PurposegRNA against the left loxP site of the Ai9 alleleDepositorInsertAi9 gRNA1
UseAAV, CRISPR, and Mouse TargetingTagsHAExpressionMutationPromoterU6Available sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-dTET1-H1672Y-D1674A-Hygro
Plasmid#104413Purposetransient expression of dCas9-dTET1 fusion proteinDepositorInsertdTET1
UseCRISPRTagsExpressionMammalianMutationH1672Y-D1674APromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCB32
Plasmid#111911PurposeExpresses gRNA to target Nte1 locus and has HygR markerDepositorInsertgRNA cassette targeting Nte1 locus
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterSNR52pAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-JMJD2A-S288G-T289G-Hygro
Plasmid#104418Purposetransient expression of dCas9-dJMJD2A fusion proteinDepositorInsertdJMJD2A
UseCRISPRTagsExpressionMammalianMutationS288G-T289GPromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX400 Campylobacter lari CF89-12 Cas9
Plasmid#68336PurposeCampylobacter lari CF89-12 Cas9DepositorInsertClCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZBTB12-donor
Plasmid#113802PurposeCRISPR donor plasmid to tag human transcription factor ZBTB12 with GFPDepositorInsertZBTB12 homology arms flanking EGFP-IRES-Neo cassette (ZBTB12 Human)
UseCRISPRTagsExpressionMutationrs9267664, rs9267663PromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
PX399 Mycoplasma gallisepticum str. F Cas9
Plasmid#68335PurposeMycoplasma gallisepticum str. F Cas9DepositorInsertMgCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTFAP2B-donor
Plasmid#113823PurposeCRISPR donor plasmid to tag human transcription factor TFAP2B with GFPDepositorInsertTFAP2B homology arms flanking EGFP-IRES-Neo cassette (TFAP2B Human)
UseCRISPRTagsExpressionMutationUnknownPromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTSHZ2-donor
Plasmid#113810PurposeCRISPR donor plasmid to tag human transcription factor TSHZ2 with GFPDepositorInsertTSHZ2 homology arms flanking EGFP-IRES-Neo cassette (TSHZ2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXD1-donor
Plasmid#112350PurposeCRISPR donor plasmid to tag human transcription factor MXD1 with GFPDepositorInsertMXD1 homology arms flanking EGFP-IRES-Neo cassette (MXD1 Human)
UseCRISPRTagsExpressionMutationhg38:2:69937537:A>C,rs3771530PromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF423-donor
Plasmid#113822PurposeCRISPR donor plasmid to tag human transcription factor ZNF423 with GFPDepositorInsertZNF423 homology arms flanking EGFP-IRES-Neo cassette (ZNF423 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 omega1 Tnos:BASTA:Pnos - P35S:hCas9:Tnos (GB3469)
Plasmid#160647Purposemodule containing the human codon optimized Cas9 and the BASTA selection markerDepositorInsertBASTA / Cas9
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnos, P35SAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZBTB11-donor
Plasmid#112348PurposeCRISPR donor plasmid to tag human transcription factor ZBTB11 with GFPDepositorInsertZBTB11 homology arms flanking EGFP-IRES-Neo cassette (ZBTB11 Human)
UseCRISPRTagsExpressionMutationhg38:3:101651181:A>TPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCB30
Plasmid#111910PurposeExpresses gRNA to target URA3 knockout locus and has KanMX markerDepositorInsertgRNA cassette targeting URA3 knockout locus
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterSNR52pAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
PX379 Legionella pneumophila str. Paris Cas9
Plasmid#68316PurposeLegionella pneumophila str. Paris Cas9DepositorInsertLpCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
EFS-FLAG-SpCas9-6xNLS(c-Myc)-P2A-Puro (pRG214)
Plasmid#221385PurposeLentiviral expression of SpCas9-6xNLS(c-Myc) with puromycin resistance geneDepositorInsertSpCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJune 11, 2024AvailabilityAcademic Institutions and Nonprofits only