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Showing: 6481 - 6500 of 20471 results
  1. pEF1a-PEmax-P2A-GFP

    Plasmid
    #202000
    Purpose
    Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.
    Depositor
    Insert
    PEmax-P2A-GFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. p-dCas9-TET1-Hygro

    Plasmid
    #104404
    Purpose
    transient expression of dCas9-TET1 fusion protein
    Depositor
    Insert
    TET1 (TET1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. p-dCas9-dTET1-H1672Y-D1674A-Hygro

    Plasmid
    #104413
    Purpose
    transient expression of dCas9-dTET1 fusion protein
    Depositor
    Insert
    dTET1
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    H1672Y-D1674A
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pCB32

    Plasmid
    #111911
    Purpose
    Expresses gRNA to target Nte1 locus and has HygR marker
    Depositor
    Insert
    gRNA cassette targeting Nte1 locus
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    SNR52p
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. p-dCas9-JMJD2A-S288G-T289G-Hygro

    Plasmid
    #104418
    Purpose
    transient expression of dCas9-dJMJD2A fusion protein
    Depositor
    Insert
    dJMJD2A
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    S288G-T289G
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. PX400 Campylobacter lari CF89-12 Cas9

    Plasmid
    #68336
    Purpose
    Campylobacter lari CF89-12 Cas9
    Depositor
    Insert
    ClCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
  7. pZBTB12-donor

    Plasmid
    #113802
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZBTB12 with GFP
    Depositor
    Insert
    ZBTB12 homology arms flanking EGFP-IRES-Neo cassette (ZBTB12 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs9267664, rs9267663
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. pTFAP2B-donor

    Plasmid
    #113823
    Purpose
    CRISPR donor plasmid to tag human transcription factor TFAP2B with GFP
    Depositor
    Insert
    TFAP2B homology arms flanking EGFP-IRES-Neo cassette (TFAP2B Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Unknown
    Promoter
    Available since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  9. pTSHZ2-donor

    Plasmid
    #113810
    Purpose
    CRISPR donor plasmid to tag human transcription factor TSHZ2 with GFP
    Depositor
    Insert
    TSHZ2 homology arms flanking EGFP-IRES-Neo cassette (TSHZ2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. pMXD1-donor

    Plasmid
    #112350
    Purpose
    CRISPR donor plasmid to tag human transcription factor MXD1 with GFP
    Depositor
    Insert
    MXD1 homology arms flanking EGFP-IRES-Neo cassette (MXD1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:2:69937537:A>C,rs3771530
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  11. pZNF423-donor

    Plasmid
    #113822
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF423 with GFP
    Depositor
    Insert
    ZNF423 homology arms flanking EGFP-IRES-Neo cassette (ZNF423 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  12. pEGB3 omega1 Tnos:BASTA:Pnos - P35S:hCas9:Tnos (GB3469)

    Plasmid
    #160647
    Purpose
    module containing the human codon optimized Cas9 and the BASTA selection marker
    Depositor
    Insert
    BASTA / Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, P35S
    Available since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
  13. pZBTB11-donor

    Plasmid
    #112348
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZBTB11 with GFP
    Depositor
    Insert
    ZBTB11 homology arms flanking EGFP-IRES-Neo cassette (ZBTB11 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:3:101651181:A>T
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  14. pCB30

    Plasmid
    #111910
    Purpose
    Expresses gRNA to target URA3 knockout locus and has KanMX marker
    Depositor
    Insert
    gRNA cassette targeting URA3 knockout locus
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    SNR52p
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
  15. EFS-FLAG-SpCas9-6xNLS(c-Myc)-P2A-Puro (pRG214)

    Plasmid
    #221385
    Purpose
    Lentiviral expression of SpCas9-6xNLS(c-Myc) with puromycin resistance gene
    Depositor
    Insert
    SpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 11, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 6481 - 6500 of 20471 results