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Plasmid#147122PurposeInsect Expression of CeAIN1_1-350DepositorInsertCeAIN1_1-350 (ain-1 Nematode)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3-DFDF-KFKF_C
Plasmid#146036PurposeInsect Expression of DmEDC3-DFDF-KFKFDepositorInsertDmEDC3-DFDF-KFKF (Edc3 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3-FDF-ADA_C
Plasmid#146037PurposeInsect Expression of DmEDC3-FDF-ADADepositorInsertDmEDC3-FDF-ADA (Edc3 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
Prham-M.XhoI-M.MunI-M.Ocy1ORF8430P-p15A-aadA
Plasmid#170768PurposeMethylase expression vector for 5RM assembly: expresses M.XhoI, M.MunI (MfeI) and M.Ocy1ORF8430P (SpeI) DNA methyltransferases in E. coliDepositorInsertsM.XhoI
M.MunI
M.Ocy1ORF8430P
UseTagsExpressionBacterialMutationPromoterrhamnoseAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMAL-ccDockerin
Plasmid#72200PurposeMBP-ccDockerin expression plasmidDepositorInsertClostridium Cellulolyticum Type I Dockerin
UseTagsHis-Tag and Maltose Binding ProteinExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOME0136
Plasmid#178398PurposeKSHV Kaposinclouster_RevCompl-KapATGdel_SynDepositorInsertKaposinclouster_RevCompl-KapATGdel_Syn
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOME0136MR
Plasmid#178399PurposeKSHV Kaposinclouster_RevCompl-KapATGdel_SynDepositorInsertKaposinclouster_RevCompl-KapATGdel_Syn
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
FKBP-EGFP-PML peptide
Plasmid#175247PurposeBacterial expression and purification, low affinity SUMOylation substrate with a relatively high Km, can be recruited to FRB with rapamycinDepositorInsertPML (PML Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-RanGAP*
Plasmid#175237PurposeBacterial expression and purification, low affinity SUMOylation substrate, point mutation F562A reduces affinity for E2, increasing the KmDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationAmino acid 562 F to APromotertacAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-E2
Plasmid#175243PurposeBacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin, CyPet acts as a FRET donor to YpetDepositorInsertUBE2I (UBE2I Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
FKBP-EGFP-RanGAP*
Plasmid#175238PurposeBacterial expression and purification, low affinity SUMOylation substrate that can recruited to FRB with rapamycin, point mutation F562A reduces affinity for E2, increasing the KmDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationAmino acid 562 F to APromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH858
Plasmid#172176PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.3f2deopt]
UseTagsExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH859
Plasmid#172177PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.JM10.1deopt]
UseTagsExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH580
Plasmid#172164PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD 3f2v2+B); AraC
UseTagsExpressionBacterialMutationPylB(N61S, E84K, E122K, E178K, G188R, E251D, G332…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH750
Plasmid#172170PurposeMicroscopy inclusion body assayDepositorInsertpCDF.3f2, deleted sumo
UseTagsExpressionBacterialMutationEvolved PylBCD operon; SUMO tag deleted from PylB…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH751
Plasmid#172171PurposeMicroscopy inclusion body assayDepositorInsertpCDF.JM10.1, deleted sumo
UseTagsExpressionBacterialMutationEvolved PylBCD operon; SUMO tag deleted from PylB…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH768v2
Plasmid#172173PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.3f2]
UseTagsExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH769v2
Plasmid#172174PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.JM10.1]
UseTagsExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH191
Plasmid#172149PurposeAccessory Plasmid for PACE of pylBCD variantsDepositorInsertPpsp gIII.2TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT
UseTagsExpressionBacterialMutationgIII(P29*, Y184*); [strong promoter eG, strong RB…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH61
Plasmid#172143PurposeAccessory Plasmid for PACE of pylBCD variantsDepositorInsertPpsp gIII.1TAG, luxAB; Plpp [eG SD8] pylS; PproK pylT
UseTagsExpressionBacterialMutationgIII(P29*); [strong promoter eG, strong RBS SD8] …PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only