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p8266 LentiCRISPR v2 hygro sgNT-1

Plasmid

#193977

Purpose

Expression of spCas9 and non-targeting control sgRNA

Depositor

Elizabeth White

Article

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

spCas9

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-on circRNA Expression Vector

Plasmid

#92351

Purpose

The vector can ef_ciently produce a circRNA (circmCherry) from a split EGFP gene after Dox treatment.

Depositor

Ling-Ling Chen

Article

Zhang et al Cell Rep. 2016 Apr 19;15(3):611-24. doi:

Insert

mCherry and split EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

pTet

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pRS327

Plasmid

#51787

Purpose

multicopy (YEp) vector with a LYS2 marker and a 2-μm origin of replication

Depositor

David Stillman

Article

Eriksson et al Biotechniques. 2004 Feb;36(2):212-3.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pHyo245

Plasmid

#173147

Purpose

Expresses pheS_A294G by dual T7 promoter in e.coli

Depositor

Seokhee Kim

Article

Park et al Nucleic Acids Res. 2021 Apr 6;49(6):e32.

Insert

pheS_A294G

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pmNFL

Plasmid

#83127

Purpose

Mouse neurofilament protein L cDNA mammalian expression construct

Depositor

Anthony Brown

Article

Yan et al Cell Motil Cytoskeleton. 2007 Apr;64(4):

Insert

mouse NFL (Nefl Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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p5E-EF1a/b-actin

Plasmid

#82583

Purpose

5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expression

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

EF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pRIP3-mCherry

Plasmid

#61386

Purpose

Expresses RIP3-mCherry in mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

RIP3 (RIPK3 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pMK262 (RAD21-mAC Neo)

Plasmid

#140538

Purpose

RAD21 tagging with mAID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

RAD21 (RAD21 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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4MT-D3-CPV

Plasmid

#58184

Purpose

Localization: Mitochondria Ca Biosensor, Excitation: 433 / 515 , Emission: 475 / 528

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pETDuet-T7-Ct-YhjH

Plasmid

#79167

Purpose

Dual expression of Ct biosensor and YhjH under individual T7 promoters

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Inserts

yhjH
T7-Spinach2-Ct

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_ATP1.0mut-IRES-mCherry-CAAX

Plasmid

#167583

Purpose

Express the ATP-insensitive control sensor GRAB_ATP1.0mut in mammalian cells

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-DsRed2

Plasmid

#15777

Depositor

Edward Callaway

Article

Wickersham et al Neuron. 2007 Mar 1. 53(5):639-47.

Insert

DsRed2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-ASAP3-WPRE

Plasmid

#132318

Purpose

expresses ASAP3(ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vector

Depositor

Michael Lin

Article

Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

Insert

ASAP3

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET-45b-mEGFP-HMGB1-WT

Plasmid

#194543

Purpose

Bacterial Expression of 6xHis mEGFP-HMGB1 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBTK527a

Plasmid

#110606

Purpose

BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with spectinomycin resistance)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST14-DogCatcher

Plasmid

#171772

Purpose

Expresses DogCatcher protein in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

DogCatcher

Use

Tags

His6 and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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Chr7_Centromere-Targeting_gRNA

Plasmid

#195129

Purpose

dual gRNA vector targeting centromere-proximal locations on Chromosome 7p and 7q in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr7 gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-3xFLAG-Rbfox3

Plasmid

#213727

Purpose

Mammalian cell expression vector for overexpression of Rbfox3 in motor neurons.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

Rbfox3 (Rbfox3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pmGFP

Plasmid

#117926

Purpose

Control plasmid expressing myc-GFP.

Depositor

Kumiko Ui-Tei

Article

Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pET15p65

Plasmid

#27661

Depositor

Kathleen Collins

Article

Prathapam et al Nat Struct Mol Biol. 2005 Mar . 12(3):25

Insert

Tetrahymena telomerase holoenzyme p65

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only
SIRT6 Flag

Plasmid

#13817

Purpose

Mammalian expression of human SIRT6 with flag tag

Depositor

Eric Verdin

Article

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT6 (SIRT6 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pCOLA-T7-WspR:G249A

Plasmid

#79163

Purpose

Expresses inactive WspR G249A under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

WspR (wspR P. areuginosa)

Use

Tags

Expression

Bacterial

Mutation

G249A

Promoter

T7

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mNeptune2.5

Plasmid

#51310

Purpose

Expresses far-red fluorescent protein mNeptune2.5 in mammalian cells

Depositor

Michael Lin

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

mNeptune2.5

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2014

Availability

Academic Institutions and Nonprofits only

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Flag-Rabin8

Plasmid

#118070

Purpose

Expresses Flag-tagged human Rabin8 in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

human Rabin8 (RAB3IP Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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LbCas12a-crRNA

Plasmid

#213753

Purpose

For circular RNA-mediated prime editor using LbCas12a-crRNA in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

LbCas12a-crRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET21TtTERT(195-516)

Plasmid

#28172

Depositor

Kathleen Collins

Article

O'Connor et al J Biol Chem. 2005 Apr 29. 280(17):17533-

Insert

Tetrahymena TERT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only
pX330A-nBRD4/PITCh

Plasmid

#91794

Purpose

PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4

Depositor

Behnam Nabet, James Bradner

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

SpCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

chicken β-actin promoter

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pET28HistevTtTERT(1-516)

Plasmid

#28171

Depositor

Kathleen Collins

Article

O'Connor et al J Biol Chem. 2005 Apr 29. 280(17):17533-

Insert

Tetrahymena TERT

Use

Tags

6xhistidine

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only
myc-hULK1

Plasmid

#31961

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

ULK1 (ULK1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV, SP6

Available since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only

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pdestMB14_sur5p-dCas9-VP64

Plasmid

#177788

Purpose

Overexpression of dCas9-VP64 in C. elegans

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

dCas9-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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