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pGEMHE-MmCENP-O-GFP

Plasmid

#205178

Purpose

Expresses mouse CENP-O C-term tagged with GFP under T7 promoter - designed for in vitro transcription

Depositor

Michael Lampson

Article

Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208

Insert

CENP-O (Cenpo Mouse)

Use

In vitro transcription

Tags

5 glycines linker followed by GFP

Expression

Mammalian

Mutation

Wild type mouse CENP-O

Promoter

T7

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pGEMHE-RnCENP-O-GFP

Plasmid

#205179

Purpose

Expresses rat CENP-O C-term tagged with GFP under T7 promoter - designed for in vitro transcription

Depositor

Michael Lampson

Article

Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208

Insert

CENP-O (Cenpo Rat)

Use

In vitro transcription

Tags

5 glycines linker followed by GFP

Expression

Mammalian

Mutation

Wild type rat CENP-O

Promoter

T7

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pGEMHE-RnCENP-O-80-GFP

Plasmid

#205182

Purpose

Expresses chimeric rat CENP-O with mouse CENP-O N-terminus; C-term tagged with GFP under T7 promoter - designed for in vitro transcription

Depositor

Michael Lampson

Article

Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208

Insert

CENP-O (Cenpo, Cenpo Mouse, Rat)

Use

In vitro transcription

Tags

5 glycines linker followed by GFP

Expression

Mammalian

Mutation

Chimeric rat CENP-O with mouse CENP-O N terminal …

Promoter

T7

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3-hARL1(Q71L)

Plasmid

#206407

Purpose

Mammalian expression of hARL1(Q71L)

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

ARL1 (ARL1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Q71L

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3-hARL1(T31N)

Plasmid

#206408

Purpose

Mammalian expression of hARL1(T31N)

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

ARL1 (ARL1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T31N

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEF mito-PAcGFP

Plasmid

#188905

Purpose

Expresses mitochondrial photoactivatable GFP

Depositor

Edwin Chapman

Article

Vevea et al J Neurosci. 2023 May 10;43(19):3421-3438

Insert

mitochondrial photoactivatable GFP

Use

Tags

Mitochondrial leader sequence: MSVLTPLLLRGLTGSARR…

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pET-GFP-mSOX17

Plasmid

#206379

Purpose

Expresses EGFP fused mouse SOX17 in bacteria, for protein production.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17 (Sox17 Mouse)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 HA pCAGGS

Plasmid

#206054

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV/B-actin

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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sfGFP-SasG(7G)-mCherry

Plasmid

#206949

Purpose

Mammalian expression of heterobifunctional SasG linkers (7G)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (7G)-mCherry

Use

Tags

mCherry and sfGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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sfGFP-SasG(6G)-mCherry

Plasmid

#206948

Purpose

Mammalian expression of heterobifunctional SasG linkers (6G)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (6G)-mCherry

Use

Tags

mCherry and sfGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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sfGFP-SasG(5G)-mCherry

Plasmid

#206947

Purpose

Mammalian expression of heterobifunctional SasG linkers (5G)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (5G)-mCherry

Use

Tags

mCherry and sfGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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sfGFP-SasG(4G)-mCherry

Plasmid

#206946

Purpose

Mammalian expression of heterobifunctional SasG linkers (4G)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (4G)-mCherry

Use

Tags

mCherry and sfGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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sfGFP-SasG(2G)-mCherry

Plasmid

#206944

Purpose

Mammalian expression of heterobifunctional SasG linkers (2G)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (2G)-mCherry

Use

Tags

mCherry and sfGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pYP-ptef1-GFP

Plasmid

#199990

Purpose

Yeast/E coli shuttle vector expressing GFP from tef1 promoter

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pET-GFP-mSOX17FNV

Plasmid

#206380

Purpose

Expresses EGFP fused mouse SOX17FNV in bacteria, for protein production.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17FNV (Sox17 Mouse)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pET-GFP-miniSOX

Plasmid

#206381

Purpose

Expresses EGFP fused miniSOX in bacteria, for protein production.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

miniSOX

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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GFP hSmad4-HomDonor

Plasmid

#206044

Purpose

Donor plasmid for CRISPR knock-in

Depositor

Ali Hemmati Brivanlou

Article

Nemashkalo et al Development. 2017 Sep 1;144(17):3042-305

Insert

Smad4 (SMAD4 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 HA pcDNA3

Plasmid

#206053

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/shFYN1/GFP4_Seq_B

Plasmid

#206135

Purpose

Knockdown of FYN

Depositor

Maria Castro

Article

Comba et al Neuro Oncol. 2020 Jun 9;22(6):806-818. d

Insert

FYN shRNA (Fyn Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/shFYN1/GFP4_Seq_A

Plasmid

#206134

Purpose

Knockdown of FYN

Depositor

Maria Castro

Article

Comba et al Neuro Oncol. 2020 Jun 9;22(6):806-818. d

Insert

FYN shRNA (Fyn Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge