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pMGF170
Plasmid#96997Purposeish1-mCherry insertion cassette with NAT resistance geneDepositorInsertish1 (ish1 Fission Yeast)
UseTagsGST and mCherryExpressionBacterialMutationish1 C-terminal domain fused with mCherry + NAT r…PromoterN/AAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-PRAS40 F129A
Plasmid#86763PurposeN-terminal HA-tagged protein expression in mammalian cellsDepositorInserthuman PRAS40 (AKT1S1 Human)
UseTagsHAExpressionMammalianMutationF129APromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-PRAS40 W10A
Plasmid#86761PurposeN-terminal HA-tagged protein expression in mammalian cellsDepositorInserthuman PRAS40 (AKT1S1 Human)
UseTagsHAExpressionMammalianMutationW10APromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-10-3xFLAG-LGP2-MIII
Plasmid#58683PurposeExpresses LGP2 with a mutation to motif III in mammalian cellsDepositorInsertLGP2-MIII (DHX58 Human)
UseTags3x FLAGExpressionMammalianMutationthreonine and serine of helicase motif III mutate…PromoterCMVAvailable sinceJuly 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
2.3 hA33 luciferase
Plasmid#68148Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -2270 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
Gal4-VP16-HSF1Δ∼PRD
Plasmid#71727Purposeplasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1Δ∼PRD RDDepositorInsertGal4 DBD HSF1 Δ∼PRD RD VP16 AD (HSF1 Human, Herpes Simplex Virus protein VP16, Budding Yeast)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.BRWD3-V5H
Plasmid#48624PurposeExpresses Drosophila BRWD3 (with V5 and His tags at the C-terminus) in mammalian cellsDepositorInsertBRWD3 (BRWD3 Fly)
UseTagsV5 and His tagsExpressionMammalianMutationno mutations, stop was removed to fuse V5His at C…PromoterCMVAvailable sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT-hMAPT
Plasmid#231583PurposeMinigene of human MAPT containing exon 9, 10, 11 and relevant intronic sequencesDepositorInserthuman MAPT (MAPT Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
10279-M28-663
Plasmid#225662PurposeLentivirus protein expression. Matched control construct for 8522-M01-663 (Addgene # 225660) gives basal expression from mCMVp for gating/thresholdingDepositorInsertdsCopGFP
UseLentiviralTagsExpressionMutationPromotermCMVpAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
10279-M30-663
Plasmid#225663PurposeLentivirus protein expression. Matched control construct for 8522-M31-663 (Addgene # 225661) gives basal expression from mCMVp for gating/thresholdingDepositorInsertdsCherry
UseLentiviralTagsExpressionMutationPromotermCMVpAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP6 145-201
Plasmid#104597PurposeExpresses C-terminal 145-201 residues of CHMP6 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-11DepositorInsertCHMP6 residues 145-201 (CHMP6 Human)
UseTagsHis-SUMOExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACB112
Plasmid#222211PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the S11R mutation and a C-terminal His tagDepositorInsertfghA(S11R)
UseTags6x HisExpressionBacterialMutationChanged Serine 11 to ArgininePromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2088-Mut-GPA-NLuc-Biosensor-Retrovirus-TD138
Plasmid#222876PurposeRetroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). Mixed WT/V84R Glycophorin A (GPA) scaffold.DepositorInsertFRB-GPA(V84R)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
UseRetroviralTagsMycExpressionMammalianMutationFRB chain: V84R in GPAPromoterMSCV LTRAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
LV-hGFAP-DLX2-ires-GFP
Plasmid#213602PurposeExpression of human DLX2 and GFP under the human GFAP ABC1D promoterDepositorInsertDLX2 (DLX2 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only
