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pGAD-Atg17(∆266-312)

Plasmid

#121521

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Use

Tags

Expression

Yeast

Mutation

deleted amino acids 266-312

Promoter

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET21d_SNORD78_probe

Plasmid

#73072

Purpose

antisense probe for detection of human SNORD78 (C/D box snoRNA U78)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD78 (SNORD78 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pUC 3GLA

Plasmid

#71762

Purpose

gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant

Depositor

Matthias Soller

Article

Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCarCBADE

Plasmid

#117757

Purpose

Codon optimized CarCBADE enzymes from Pectobacterium carotovorum in pBbaA5k backbone

Depositor

Greg Bokinsky

Article

Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

Insert

Codon optimised CarCBA, Protein CarD, Ferredoxin CarE

Use

Tags

Expression

Bacterial

Mutation

Promoter

PLac(UV5)

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCDB367

Plasmid

#110277

Purpose

gHEEE_02 with a N-terminal 10-His DsbC TEV-peptide fusion

Depositor

Christopher Bahl

Article

Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

Insert

gHEEE_02

Use

Tags

10-His, Tev recognition peptide, and mature DsbC …

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pGAL4DBD-SuUR f.l

Plasmid

#112995

Purpose

P element vector for transposon insertion of full-length Drosophila SuUR into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Sher et al Genome Res. 2012 Jan;22(1):64-75. doi: 1

Insert

SuUR (SuUR Fly)

Use

P insertion vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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miR-223-RFP pCDNA

Plasmid

#97142

Purpose

expression of miR-223 and TagRFP

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

miR-223

Use

Tags

TagRFP

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSI-check2-TAB2-3'UTR

Plasmid

#97157

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

TAB2 3'UTR (TAB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1291 TDP43 RRM-GFP G368W

Plasmid

#107837

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

G368W mutation in LLPS reporter

Promoter

Available since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS934 TDP43 RRM-GFP M-V

Plasmid

#107801

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All methionine to valine in LLPS reporter

Promoter

Available since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS935 TDP43 RRM-GFP M-S

Plasmid

#107800

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All methionine to serine in LLPS reporter

Promoter

Available since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1052 TDP43 RRM-GFP R-K

Plasmid

#107856

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All arginine to lysine in LLPS reporter

Promoter

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1279 TDP43 RRM-GFP G348V

Plasmid

#107831

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

G348V mutation in LLPS reporter

Promoter

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1280 TDP43 RRM-GFP G348F

Plasmid

#107830

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

G348F mutation in LLPS reporter

Promoter

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS968 TDP43 RRM-GFP G309S

Plasmid

#107824

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

G309S mutation in LLPS reporter

Promoter

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS966 TDP43 RRM-GFP G309F

Plasmid

#107822

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

G309F mutation in LLPS reporter

Promoter

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1256 TDP43 RRM-GFP W385G

Plasmid

#107807

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

W385G mutation in LLPS reporter

Promoter

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pGS_C_3xFLAG

Plasmid

#101653

Purpose

Expression of type I signal-peptide containing proteins with C-terminal 3xFLAG-tag in insect cells

Depositor

Monika Golas, Bjoern Sander

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, MCS linker, 3xFLAG tag

Use

Tags

3xFLAG tag

Expression

Insect

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMflT-o2

Plasmid

#101310

Purpose

Contains: oriC2 fragment of M. florum L1 replication origin (dnaA/dnaN intergenic region), colE1 replication origin, recoded tetM resistance gene.

Depositor

Sébastien Rodrigue

Article

Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

Insert

oriC2 of M. florum strain L1 (dnaA/dnaN intergenic region)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 S137D (#628)

Plasmid

#99944

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S137D

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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