Addgene

Showing: 3231 - 3240 of 10528 results

Results per page:

Sort results by:

pcdna6.2- myc ULK1 wt

Plasmid

#27629

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

mouse myc ULK1 (Myc Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Initial M from ULK1 removed. Silent mutation at A…

Promoter

Available since

Feb. 18, 2011

Availability

Academic Institutions and Nonprofits only
pMG058 MBP-GS 634-666-His

Plasmid

#154077

Purpose

Expresses MBP-GS 634-666-His (human 33aa Glycogen Synthase peptide as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

GYS1 (GYS1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-TetO-Sox17

Plasmid

#128828

Purpose

dox-dependent expression of mouse Sox17

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Sox17 (Sox17 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPICZ-mouseGIRK2-eGFP-StII-6xHis

Plasmid

#124277

Purpose

Expresses mouse GIRK2 as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

Depositor

Arthur Laganowsky

Article

Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

Insert

GIRK2 (Kcnj6 Mouse)

Use

Tags

eGFP-StreptagII-6xHis

Expression

Yeast

Mutation

Promoter

AOX1

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_ 2xFLAG-2xSTREP_BACH1_Y11F

Plasmid

#159132

Purpose

Expresses BACH1 in mammalian cells

Depositor

Michele Pagano

Article

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Insert

BACH1 (BACH1 Human)

Use

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Mutation

Y11F

Promoter

CMV

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-H1-shRNA-CMV-HA-PACSIN1

Plasmid

#72577

Purpose

Bicistronic construct expressing PACSIN1 shRNA and HA-PACSIN1-rescue construct

Depositor

Richard Huganir

Article

Anggono et al Proc Natl Acad Sci U S A. 2013 Aug 20;11

Inserts

PACSIN1 (Pacsin1 Mouse)
H1-PACSIN1 shRNA cassette

Use

RNAi

Tags

HA

Expression

Mammalian

Mutation

5 silent mutations around shRNA recognition seque…

Promoter

CMV and H1

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH130

Plasmid

#162484

Purpose

SEC plasmid containing LG1 homology arms and myo-3p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.

Depositor

Bob Goldstein

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

myo-3p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR

Use

CRISPR

Tags

myo-3p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR

Expression

Worm

Mutation

Promoter

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
OA-1136J

Plasmid

#153023

Purpose

Express His-MBP-TEV-CasRx recombinant protein

Depositor

Omar Akbari

Article

Brogan et al ACS Sens. 2021 Nov 26;6(11):3957-3966. d

Insert

CasRx

Use

Tags

His-MBP-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH126

Plasmid

#162482

Purpose

SEC plasmid containing LG1 homology arms and vha-8p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.

Depositor

Bob Goldstein

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

vha-8p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR

Use

CRISPR

Tags

vha-8p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR

Expression

Worm

Mutation

Promoter

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis

Plasmid

#124278

Purpose

Expresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

Depositor

Arthur Laganowsky

Article

Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

Insert

GIRK2 (Kcnj6 Mouse)

Use

Tags

eGFP-StreptagII-6xHis

Expression

Yeast

Mutation

R201A

Promoter

AOX1

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge