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Plasmid#110641PurposeRetroviral expression of flag-tagged yeast FSF1 in mammalian cellsDepositorInsertFSF1 (FSF1 Budding Yeast)
UseRetroviralTagsFLAGExpressionMammalianMutationcodon-optimized cDNA (no amino acid mutations)PromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBW1309_pCAG-PV1-FKBP-L1-CTD-ENTRY-BGHpA
Plasmid#108837PurposeInsert protein fragment into KpnI/EcoRI to fuse to FKBP tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsFKBPExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1312_pCAG-PV1-NTD-ENTRY-L1-ABI-NLS-BGHpA
Plasmid#108840PurposeInsert protein fragment into MluI/BspEI to fuse to ABI/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsABI,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1313_pCAG-PV1-PYL-L1-CTD-ENTRY-BGHpA
Plasmid#108841PurposeInsert protein fragment into KpnI/EcoRI to fuse to PYL tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsPYLExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2654_pCAG-PV1-pMag-L1-CTD-ENTRY-BGHpA
Plasmid#108847PurposeInsert protein fragment into KpnI/EcoRI to fuse to pMag tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagspMagExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1308_pCAG-PV1-NTD-ENTRY-L1-FRB-NLS-BGHpA
Plasmid#108836PurposeInsert protein fragment into MluI/BspEI to fuse to FRB/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSMP-MeCP2_1
Plasmid#36377DepositorInsertMeCP2 (MECP2 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBW2064_pCAG-PV1-NTD-ENTRY-L1-GAI-NLS-BGHpA
Plasmid#108842PurposeInsert protein fragment into MluI/BspEI to fuse to GAI/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsGAI,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM1157
Plasmid#112534PurposepZE2-PLhrtO-lux-hrtR-RBS3 - ChuA transciptional unit deletion of plasmid pMM643, ColE1 origin, KanRDepositorInsertsHrtR
luxCDABE
UseTagsExpressionBacterialMutationPromoterPL(HrtO) and ProDAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-Cre stuffer v4
Plasmid#158033Purposelenti-viral construct with Cas9, Cre recombinase and U6 driven sgRNA casette with modified tracr RNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
MBP-TSF-TEVcs-ULK1K46I
Plasmid#171417PurposeFor the purifcation of human ULK1 kinase dead complexDepositorInsertULK1 (ULK1 Synthetic)
UseTagsExpressionMammalianMutationkinase deadPromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
KTK_247
Plasmid#180529PurposeEntry vector containing pTac promoterDepositorInsertpTac promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDB181
Plasmid#80692Purposefor secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEHEE_06DepositorInsertgEHEE_06
UseTagsOsmY-10His-Smt3ExpressionBacterialMutationPromoterT7Available sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(human)(R332P)
Plasmid#79037PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(human R332P) (TRIM5 Human)
UseTagsOneSTrEP-FLAGExpressionInsectMutationChanged Arg 332 to ProPromoterpolyhedrinAvailable sinceMarch 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 delta 610-615
Plasmid#19941DepositorInsertcullin 1 delta 610-615 (CUL1 Human)
UseTagsHAExpressionMammalianMutationCUL1 with a ROC1 binding mutantPromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-SLC35B2-FLAG
Plasmid#154863PurposeTransient or Lentiviral expression of sgRNA (Addgene#154860) resistant SLC35B2DepositorInsertSLC35B2 (SLC35B2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationctcctggtgcagtacttc => ttattagtccaatattttPromoterCMVAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pDGB3 alpha1 P35S:AsCas12a:T35S (GB1440)
Plasmid#160610PurposeTranscriptional unit for the expression of the AsCas12a in plant systems driven by the CaMV35s promoter.DepositorInsertP35s:AsCas12a:T35s
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterP35SAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMEH60 Lamp2b MS2 HA
Plasmid#168209PurposeMammalian expression of lysosome-associated membrane protein 2b fused to the MS2 dimer and C-terminal HA tagDepositorInsertLamp2b (LAMP2 Human)
UseTagsHA and MS2 dimerExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSMP-MeCP2_3
Plasmid#36379DepositorInsertMeCP2 (MECP2 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only