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Plasmid#154074PurposeExpresses GST_CK1α (human CK1α as a fusion protein with GST and His- tags) in E.coliDepositorInsertCSNK1A1 (CSNK1A1 Human)
UseTagsGST and His6ExpressionBacterialMutationPromoterT7Available sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EphrinB3-Flag
Plasmid#155012PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 from pcDNA3.1DepositorInsertEphrinB3 (EPHB3 Human)
UseTagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNMSB84
Plasmid#199309PurposeMosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605DepositorInsertflp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
UseTagsExpressionWormMutationmTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…Promoterflp-18Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNMSB99
Plasmid#199308PurposeMosSCI insertion of myo-3p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605DepositorInsertmyo-3p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
UseTagsExpressionWormMutationmTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…Promotermyo-3Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Cdx2
Plasmid#128829Purposedox-dependent expression of mouse Cdx2DepositorInsertCdx2 (Cdx2 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
GLuc-PM
Plasmid#164783PurposeGaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptorsDepositorInsertGaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Gaussia princeps, Human)
UseTagsGLucExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ 2xFLAG-2xSTREP_FBXO22
Plasmid#159127Purposeexpresses FBXO22 in mammalian cellsDepositorInsertFBXO22 (FBXO22 Human)
UseTags2X-FLAG _ 2X-STREPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Tead4
Plasmid#128832Purposedox-dependent expression of mouse Tead4DepositorInsertTead4 (Tead4 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-hCD2-CD14 (4R)
Plasmid#133062PurposeRetroviral expression of mouse CD14 mutant "4R" deficient in LPS bindingDepositorInsertCD14 (4R) (Cd14 Mouse)
UseRetroviralTagsExpressionMammalianMutationCD14 allele defective for LPS binding (mutations …PromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF073 MBP-Axin-His
Plasmid#154011PurposeExpresses MBP-Axin-His (human Axin as a fusion protein with MBP and His- tags) in E.coliDepositorInsertAxin1 (AXIN1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromotertacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only