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Plasmid#127328PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon from mRNA with BAG1 5' UTRDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL509 AT sgRNA
Plasmid#126429PurposeTo target a protospacer with AT at the cut site.DepositorInsertspacer against human genome with AT at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL322 GC
Plasmid#126950PurposeTo target a protospacer with GC at the cut site.DepositorInsertspacer against human genome with GC at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL581 GA
Plasmid#126951PurposeTo target a protospacer with GA at the cut site.DepositorInsertspacer against human genome with GA at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL601 AG
Plasmid#126952PurposeTo target a protospacer with AG at the cut site.DepositorInsertspacer against human genome with AG at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL602 CG
Plasmid#126953PurposeTo target a protospacer with CG at the cut site.DepositorInsertspacer against human genome with CG at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB153 - pL2_pSB90_2x35S::ZCT1::tMAS
Plasmid#123195Purposebinary plant vector for transient expression of ZCT1 from Catharanthus roseusDepositorInsert2x35S::ZCT1::tMAS
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB160 - pL2_pSB90_2x35S::ORCA3::tMAS
Plasmid#123196Purposebinary plant vector for transient expression of ORCA3 from Catharanthus roseusDepositorInsert2x35S::ORCA3::tMAS
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB166 - pL2_pSB90_tMAS::rLUC-I::pMAS
Plasmid#123199Purposebinary plant vector for transient expression of a Renilla luciferase (rLUC, with intron)DepositorInserttMAS::rLUC-I::pMAS
UseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJET1.2_Rab28_Sense
Plasmid#124441PurposePlasmid for sense in situ probe in vitro transcriptionDepositorInsertRAB28, member RAS oncogene family (Rab28 Mouse)
UseIn situ probeTagsExpressionMutationPromoterT7Available sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-941
Plasmid#103757PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-941 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-941 target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-567
Plasmid#103650PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-567 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorUseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-1
Plasmid#103160PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-1 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-1 target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pDONR221-OSF-TRIM5-21R(1-300)
Plasmid#79029PurposeGateway entry vector for cloning and propagation in DH5alphaDepositorInsertTRIM5-21R (1-300)
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterT7Available sinceMarch 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIMCyp (owl monkey)
Plasmid#79035PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5Cyp (owl monkey)
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterpolyhedrinAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNIA-CEN-FLAG-LINXa4-Bre1
Plasmid#73617PurposeControl of the E3 ligase Bre1 with LINXa in yeast.DepositorInsertLINXa4-Bre1dNLS
UseSynthetic BiologyTagsFLAGExpressionYeastMutationBre1 point mutations K8A, K9A and K11APromoterADH1Available sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-mOC-STAMP-GFP
Plasmid#73579PurposeLentivirus expression of mOC-STAMPDepositorInsertOC-STAMP (Ocstamp Mouse)
UseLentiviralTagsGFPExpressionMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGS1613
Plasmid#63789PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal tDimer2 Strep-tag II followed by HIS5 selection markerDepositorInserttDimer2-Strep-tag II followed by HIS5
UseE. coli cloning vectorTagstDimer2-Strep-tag IIExpressionMutationPromoternoneAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-gRNA:kit-1
Plasmid#60969PurposeExpression vector of rat Kit-1 guide RNADepositorInsertv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog gRNA (Kit Rat)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 5, 2015AvailabilityAcademic Institutions and Nonprofits only