Showing: 6421 - 6440 of 16015 results
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Plasmid#114077PurposeT7 promoter bacterial expression plasmid for human codon optimized AsCas12a-enRR(E174R/S542R/K607R) with C-terminal NLS and His-tagDepositorInserthuman codon optimized AsCas12a-enRR (E174R/S542R/K607R) with C-terminal NLS and His-tag
UseTagsNLS(nucleoplasmin) and 6xHisExpressionBacterialMutationE174R/S542R/K607RPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTO_CHMP2B_LAP
Plasmid#101848PurposeTet-inducible expression of human CHMP2B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integrationDepositorInsertCHMP2B (CHMP2B Human)
UseFlp-frt recombination, tet-inducible expressionTagsLocalization and Affinity Purification (LAP) tagExpressionBacterial and MammalianMutationsilent mutation in Thr 104; A to TPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pTO_CHMP4B_LAP
Plasmid#101850PurposeTet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integrationDepositorInsertCharged Multivesicular Body Protein 4B (CHMP4B Human)
UseGateway cloning, flp-frt recombination, tet-induc…TagsLocalization and Affinity Purification (LAP) tagExpressionBacterial and MammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Gi2-CASE
Plasmid#168121PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi2. Composed of the subunits G alpha i2 (GNAI2) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at C112/E115 within GNAI2PromoterAvailabilityAcademic Institutions and Nonprofits only -
Gq-CASE
Plasmid#168125PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gq. Composed of the subunits G alpha q (GNAQ) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at F124/E125 within GNAQPromoterAvailabilityAcademic Institutions and Nonprofits only -
G13-CASE
Plasmid#168127PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at F125/D126 within GNA13PromoterAvailabilityAcademic Institutions and Nonprofits only -
Go1-CASE
Plasmid#168123PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at L91/G92 within GNAO1PromoterAvailabilityAcademic Institutions and Nonprofits only -
Gs(short)-CASE
Plasmid#168124PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).DepositorUseLuciferaseTagscpVenus on GNG1ExpressionMammalianMutationNLuc is inserted at N136/V137 within GNASPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-SpSuc1
Plasmid#162558PurposeExpresses GST-tagged S.pombe Suc1 in E.coliDepositorInsertSchizosaccharomyces pombe Suc1 (suc1 Fission Yeast)
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Hygro-Bem1-mCherry-AID
Plasmid#173925PurposePlasmid designed for the genomic tagging of saccharomyces cerevisiae Bem1 with mCherry-AIDDepositorInsertsPartial Bem1 C-terminus
hygromycin marker
Bem1 downstream sequence
UseTagsmCherry- AIDExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
APRT
Plasmid#64028PurposeExpression of S. crervisiae strain APRTase in E. coli. Contains N-terminal HistidineX6 tag.DepositorInsertadenine phosphoribosyltransferase (APT1 Budding Yeast)
UseTagsHistidine x 6ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMEXNH3
Plasmid#110079PurposeAnalogous to pMINTNH3, but containing oriM for episomal replication in mycobacteria.DepositorTypeEmpty backboneUseTagsHis10 Tag - 3C cleavage siteExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMEXC3H
Plasmid#110080PurposeAnalogous to pMINTC3H, but containing oriM for episomal replication in mycobacteria.DepositorTypeEmpty backboneUseTags3C cleavage site - His10 TagExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
ClhN-4V5-APEX2-URA
Plasmid#207056PurposeFor ORF tagging with 4V5-APEX2DepositorInsert4xV5-APEX2
UseTags4xV5-APEX2ExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSKDuet21
Plasmid#41695DepositorInsertPhoCDC21-1 intein
UseTagsGB1 and His-tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pSKDuet22
Plasmid#41696DepositorInsertPhoCDC21-2 intein
UseTagsGB1 and His-tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pSKDuet23
Plasmid#41697DepositorInsertPhoPol-II intein
UseTagsGB1 and His-tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pNIC-NH10-GG
Plasmid#215806PurposeCloning of target gene with N terminal non cleavable His10 tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-CH10-GG
Plasmid#215807PurposeCloning of target gene with C terminal non cleavable His10 tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pFM689
Plasmid#74635Purposealias pFA6a-mCherry-hphNT1 - vector for C-terminal tagging of yeast genes with mCherry and hygromycin selection markerDepositorInsertsUseTagsExpressionYeastMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only
Showing: 6421 - 6440 of 16015 results