Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 6401 - 6420 of 16015 results

  1. TBXTA-c044

    Plasmid
    #139767
    Purpose
    Protein expression/Streptavidin pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Use
    Tags
    Avi-tag (Biotin) and His6-TEV
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41- D225
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    139767-plasmid-map-sequence-id-296582

    Enlarge

  2. USP37

    Plasmid
    #36898
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP37 (USP37 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    contains amino acid residues 4-125 only
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. pSBbi-Puro_LPAR_AKAR-mut

    Plasmid
    #125207
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Eric Davis
    Insert
    LPAR_AKAR-mut
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Availability
    Academic Institutions and Nonprofits only
    125207-plasmid-map-sequence-id-243606

    Enlarge

  4. pLKO.1 LARP7-FLAG 7SK

    Plasmid
    #200733
    Purpose
    Lentiviral expression of Flag-Tagged human LARP7 and the snRNA 7SK
    Depositor
    Claudio Alarcón
    Insert
    LARP7 and 7SK (LARP7 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    200733-plasmid-map-sequence-id-401803

    Enlarge

  5. HARS_pNIC-Bio3

    Plasmid
    #153050
    Purpose
    Expression construct for producing Avi tagged histidyl tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    HARS (HARS1 Human)
    Use
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    153050-plasmid-map-sequence-id-304465

    Enlarge

  6. pIRES-H3.3-G34R-FLAG-BFP

    Plasmid
    #206140
    Purpose
    Expression of a FLAG tagged human histone mutant H3.3-G34R with BFP
    Depositor
    Maria Castro
    Insert
    H3.3 (H3-3A Human)
    Use
    Tags
    FLAG-eBFP
    Expression
    Mammalian
    Mutation
    G34R
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    206140-plasmid-map-sequence-id-405579

    Enlarge

  7. pCAG-EGFP-N-IFT80

    Plasmid
    #218731
    Purpose
    Expresses C-terminally EGFP-tagged IFT80 in mammalian cells
    Depositor
    Kazuhisa Nakayama
    Insert
    IFT80 (IFT80 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    218731-plasmid-map-sequence-id-434218

    Enlarge

  8. pAcGFP-N1_CBPdIDR1

    Plasmid
    #221904
    Purpose
    Transient expression of GFP-tagged CBPdeltaIDR1 in mammalian cells
    Depositor
    Daniel Bose
    Insert
    CBPdeltaIDR1 (CREBBP Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    deletion of IDR1 (aa 72-175)
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    221904-plasmid-map-sequence-id-437441

    Enlarge

  9. UHRF1

    Plasmid
    #28163
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    UHRF1 (UHRF1 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    tandem tudor domain
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. KARS_pNIC-Bio3

    Plasmid
    #153051
    Purpose
    Expression construct for producing Avi tagged Lysyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    KARS (KARS1 Human)
    Use
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    153051-plasmid-map-sequence-id-304466

    Enlarge

  11. pAcGFP-N1_CBPdIDR3

    Plasmid
    #221905
    Purpose
    Transient expression of GFP-tagged CBPdeltaIDR3 in mammalian cells
    Depositor
    Daniel Bose
    Insert
    CBPdeltaIDR3 (CREBBP Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    deletion of IDR3 (aa 441-587)
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    221905-plasmid-map-sequence-id-437439

    Enlarge

  12. pAcGFP-N1_CBPdIDR4

    Plasmid
    #221906
    Purpose
    Transient expression of GFP-tagged CBPdeltaIDR4 in mammalian cells
    Depositor
    Daniel Bose
    Insert
    CBPdeltaIDR4 (CREBBP Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    deletion of IDR4 (aa 662-787)
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    221906-plasmid-map-sequence-id-437458

    Enlarge

  13. pAcGFP-N1_CBPdIDR6

    Plasmid
    #221907
    Purpose
    Transient expression of GFP-tagged CBPdeltaIDR6 in mammalian cells
    Depositor
    Daniel Bose
    Insert
    CBPdeltaIDR6 (CREBBP Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    deletion of IDR6 (aa 1856-2090)
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    221907-plasmid-map-sequence-id-437459

    Enlarge

  14. pAcGFP-N1_CBPdIDR7

    Plasmid
    #221908
    Purpose
    Transient expression of GFP-tagged CBPdeltaIDR7 in mammalian cells
    Depositor
    Daniel Bose
    Insert
    CBPdeltaIDR7 (CREBBP Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    deletion of IDR7 (aa 2109-2434)
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    221908-plasmid-map-sequence-id-437440

    Enlarge

  15. pET-28b-T7-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-6xHis (AAS1880)

    Plasmid
    #114071
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a(E174R/S542R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a (E174R/S542R) with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R and S542R
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    114071-plasmid-map-sequence-id-220509

    Enlarge

  16. pET-28b-T7-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-6xHis (AAS1916)

    Plasmid
    #114076
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-RR (S542R/K607R) with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    S542R and K607R
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    114076-plasmid-map-sequence-id-220531

    Enlarge

  19. pTO_CHMP2B_LAP

    Plasmid
    #101848
    Purpose
    Tet-inducible expression of human CHMP2B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Article
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    CHMP2B (CHMP2B Human)
    Use
    Flp-frt recombination, tet-inducible expression
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Mutation
    silent mutation in Thr 104; A to T
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    101848-plasmid-map-sequence-id-200235

    Enlarge

  20. pTO_CHMP4B_LAP

    Plasmid
    #101850
    Purpose
    Tet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Article
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    Charged Multivesicular Body Protein 4B (CHMP4B Human)
    Use
    Gateway cloning, flp-frt recombination, tet-induc…
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    101850-plasmid-map-sequence-id-200248

    Enlarge

Showing: 6401 - 6420 of 16015 results