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pLD-puro-Cc-PRDX5K85R-VA

Plasmid

#98691

Purpose

Lentiviral expression of human PRDX5-K85R in mammalian cells

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

K85R

Promoter

CMV

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-PRDX5E80K-VA

Plasmid

#98689

Purpose

Lentiviral expression of human PRDX5-E80K in mammalian cells

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

E80K

Promoter

CMV

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-PRDX5K83R-VA

Plasmid

#98690

Purpose

Lentiviral expression of human PRDX5-K83R in mammalian cells

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

K83R

Promoter

CMV

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLCS111

Plasmid

#209332

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter expression of N-terminal mScarlet-I-mNeonGreen tandem fluorescent timer fusion protein.

Depositor

Lucia Strader

Article

Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHEN2-SG4 R105A

Plasmid

#196072

Purpose

Express mutant (R105A) SG4 in E.coli

Depositor

Shankar Balasubramanian

Article

Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

Insert

SG4 R105A

Use

Tags

3XFLAG and 6XHis

Expression

Bacterial

Mutation

R105A mutation in SG4

Promoter

T7

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCX-SpiCee

Plasmid

#140836

Purpose

mammalian expression of SpiCee, a buffer of calcium that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

SpiCee

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCX-mutSpiCee

Plasmid

#140903

Purpose

mammalian expression of a mutated form of SpiCee, that does not affect calcium signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

mutSpiCee

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+ Lifeact-mCherry

Plasmid

#185408

Purpose

Red fluorescent reporter for polymerized actin. For in vitro transcription (SP6) or expression through CMV

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

lifeact

Use

In vitro synthesis of mrna

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual- GST-TEV-TBK1

Plasmid

#208875

Purpose

For expression in insect cells of TBK1

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

TBK1 (TBK1 Human)

Use

Tags

GST-TEV

Expression

Insect

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEcCas

Plasmid

#73227

Purpose

Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA

Depositor

Sheng Yang

Article

Li et al Acta Biochim Biophys Sin (Shanghai). 202

Inserts

Cas9
sgRNA

Use

CRISPR; Red recombinase

Tags

Expression

Bacterial

Mutation

Promoter

native promoter

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt3

Plasmid

#35909

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt3 (WNT3 Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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LentiV_Blast

Plasmid

#111887

Purpose

Lentiviral expression of cDNA with blasticidin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Type

Empty backbone

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EFS promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIP CMV GFP1-9

Plasmid

#130271

Purpose

Lentiviral expression vector for stable expression of GFP1-9

Depositor

Stéphanie Cabantous

Article

Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

Insert

GFP 1-9 OPT

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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MStart-36xMashTag-BFP-3xStop-24xPP7 (Moonstart-MashTag reporter)

Plasmid

#128607

Purpose

Expresses 36xMashTag with MoonTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

36xMashTag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Pcmv

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAGM65879

Plasmid

#153214

Purpose

Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and Bar selection cassette

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNASAM(gEmpty)-TREGFP-PGKpuroBFP-W

Plasmid

#112926

Purpose

CRISPR-a reporter assay lentiviral vector (empty gRNA control)

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6-gRNASAM(empty), TREGFP, PGKpuroGFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSK-mU6-Cj-sgRNA-SV40-puro

Plasmid

#192128

Purpose

Expresses Cj-SgRNA scaffold cloned with BbsI and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Cj-SgRNA scaffold

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-HA-hM3D(Gq)

Plasmid

#45547

Depositor

Bryan Roth

Article

Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

Insert

HA-hM3D

Use

Tags

HA

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

July 3, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-HA-hM4D(Gi)

Plasmid

#45548

Depositor

Bryan Roth

Article

Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

Insert

HA-hM4D

Use

Tags

HA

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-HA-rM3D(Gs)

Plasmid

#45549

Depositor

Bryan Roth

Article

Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

Insert

HA-rM3D

Use

Tags

HA

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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