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RfxCas13d-backbone

Plasmid

#165078

Purpose

Expression of RfxCas13d sgRNAs from a U6 promoter

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

Scaffold gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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PLJR962

Plasmid

#115162

Purpose

Sth1 dCas9 TetR and KanR L5 Int attP for M. smegmatis

Depositor

Sarah Fortune

Article

Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

Inserts

Sth1 sgRNA scaffold
Sth1 dCas9
TetR
L5 attP
L5 Int
KanR

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pNL-CEF-CD79B-RFP

Plasmid

#187005

Purpose

Expression of human CD79B as RFP fusion protein

Depositor

Hermann Eibel

Article

Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

Insert

CD79B (CD79B Human)

Use

Lentiviral

Tags

tagRFP

Expression

Mutation

Promoter

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV_S5E2_Chrimson_tdTomato

Plasmid

#219432

Purpose

AAV construct expressing Chrimson-tdTomato under the control of E2 regulatory element

Depositor

Gordon Fishell

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

Chrimson-tdTomato

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pSB700

Plasmid

#64046

Purpose

Lentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent protein

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

U6, CAGGS

Available since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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Px461-Cas9n-Trp53-sgRNA-beta

Plasmid

#88847

Purpose

CRISPR KO of Trp53

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp53 (Trp53 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only
pCDNA3.1(+) miniTurboID-6xGGSG linker-FKBPF36V-2xHA

Plasmid

#200641

Purpose

Expresses miniTurboID-FKBPF36V-2xHA in mammalian cells.

Depositor

Fleur Ferguson, Justin English

Article

Tao et al Nat Commun. 2023 Dec 4;14(1):8016. doi:

Insert

miniTurboID-6xGGSG linker-FKBPF36V-2xHA

Use

Tags

HA-tag

Expression

Mammalian

Mutation

Promoter

T7

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-OSKM

Plasmid

#20321

Purpose

Lentiviral plasmid for tet-inducible expression of mouse Oct4, Sox2, Klf4 and Myc for iPS cell generation

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Oct-4 (Pou5f1, Sox2, Klf4, Myc Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pCRISPRx-Sth1Cas9-L5

Plasmid

#140993

Purpose

Sth1Cas9, TetR, KanR, L5Int, attP to create frameshifts and gene deletion in Mycobacteria

Depositor

Wilbert Bitter

Article

Meijers et al Tuberculosis (Edinb). 2020 Sep;124:10198

Inserts

Sth1Cas9
TetR
L5 attP
L5 Int
KanR

Use

Unspecified; Integrative in mycobactreia

Tags

Expression

Mutation

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pET20b-NLuc-MBP-6xHis

Plasmid

#135931

Purpose

Expresses NanoLuciferase in bacterial cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

NanoLuciferase

Use

Tags

MBP-6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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