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ORF8-1x-TurboID pbabe-puro (SARS-CoV-2)

Plasmid

#185993

Purpose

Expresses SARS-CoV-2 ORF8 to TurboID and 3xHA tag

Depositor

Kyle Roux

Article

May et al Viruses. 2022 Mar 15;14(3). pii: v140306

Insert

SARS-CoV-2 ORF8 (ORF8 SARS-CoV-2)

Use

Retroviral

Tags

3xHA and TurboID

Expression

Mammalian

Mutation

Promoter

LTR

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-HIF2α P405A/P531A/N847A-pBabe-Puro

Plasmid

#25956

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Promoter

Available since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE-Flag-Cdk9-D167N-IRES-eGFP

Plasmid

#28098

Depositor

Andrew Rice

Article

Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

Insert

Cdk9 (CDK9 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Contains D167N mutation in Cdk9

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only
pBabe-TetCMV-puro-mVenus-PA-Rac1-C450M

Plasmid

#22034

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mVenus-PA-Rac1-C450M (RAC1 Human, Avena sativa (oat))

Use

Retroviral

Tags

Expression

Mammalian

Mutation

The LOV domain contains the mutation C450M.Rac1 s…

Promoter

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
HA-HIF2α P405A Δ450-572-pBabe-Puro

Plasmid

#25958

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Promoter

Available since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A

Plasmid

#22023

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mVenus-PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))

Use

Retroviral

Tags

Expression

Mammalian

Mutation

The LOV domain contains the mutation C450A.Rac1 s…

Promoter

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pBabe puro-HA-PIK3CA(H1047R;F486S;del)

Plasmid

#33255

Depositor

Thomas Roberts

Article

Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

Hemagglutinin (HA)

Expression

Mammalian

Mutation

changed histidine 1047 to arginine and phenylalan…

Promoter

SV40

Available since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only
pBABE-Flag-Cdk9-T186A-IRES-eGFP

Plasmid

#28097

Depositor

Andrew Rice

Article

Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

Insert

Cdk9 (CDK9 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Contains T186A mutation in Cdk9

Promoter

Available since

April 15, 2011

Availability

Academic Institutions and Nonprofits only
NSP13-1x-BioID2 pBabe-puro (SARS-CoV-2)

Plasmid

#186007

Purpose

Expresses SARS-CoV-2 NSP13 fused to BioID2 and HA tag

Depositor

Kyle Roux

Article

May et al Viruses. 2022 Mar 15;14(3). pii: v140306

Insert

SARS-CoV-2 NSP13 (ORF1ab SARS-CoV-2)

Use

Retroviral

Tags

BioID2 and HA

Expression

Mammalian

Mutation

Promoter

LTR

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
NSP9-1x-BioID2 pBabe-puro (SARS-CoV-2)

Plasmid

#186003

Purpose

Expresses SARS-CoV-2 NSP9 fused to BioID2 and HA tag

Depositor

Kyle Roux

Article

May et al Viruses. 2022 Mar 15;14(3). pii: v140306

Insert

SARS-CoV-2 NSP9 (ORF1ab SARS-CoV-2)

Use

Retroviral

Tags

BioID2 and HA

Expression

Mammalian

Mutation

Promoter

LTR

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-HIF2α P405A/P531A/N847Q-pBabe-Puro

Plasmid

#25948

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Promoter

Available since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe-TetCMV-puro-mVenus-PA-Rac1-T17N

Plasmid

#22018

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mVenus-PA-Rac1-T17N (RAC1 Human, Avena sativa (oat))

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Rac1 starts at I4 and contains mutation T17N.

Promoter

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pBabe-TetCMV-puro-mPA-GFP-PA-Rac1

Plasmid

#22032

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mPA-GFP-PA-Rac1 (RAC1 Human, Avena sativa (oat))

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Promoter

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
BioID2-1x-ORF7b pBabe-puro (SARS-CoV-2)

Plasmid

#185992

Purpose

Expresses BioID2 fused to SARS-CoV-2 ORF7b and myc tag

Depositor

Kyle Roux

Article

May et al Viruses. 2022 Mar 15;14(3). pii: v140306

Insert

SARS-CoV-2 ORF7b (ORF7b SARS-CoV-2)

Use

Retroviral

Tags

BioID2 and myc

Expression

Mammalian

Mutation

Promoter

LTR

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
ORF7a-1x-BioID2 pBabe-puro (SARS-CoV-2)

Plasmid

#185991

Purpose

Expresses SARS-CoV-2 ORF7a fused to BioID2 and HA tag

Depositor

Kyle Roux

Article

May et al Viruses. 2022 Mar 15;14(3). pii: v140306

Insert

SARS-CoV-2 ORF7a (ORF7a SARS-CoV-2)

Use

Retroviral

Tags

BioID2 and HA

Expression

Mammalian

Mutation

Promoter

LTR

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
1130 pBabe puroL HA FKHR H215R AAA

Plasmid

#9035

Depositor

William Sellers

Article

Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

Insert

FKHR H215R AAA (FOXO1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

H215R T24A S256A S319A

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pBabe-Tet-off-puro-mCherry-PA(wt)-Vav2

Plasmid

#91873

Purpose

Photo-activatable/light sensitive Vav2

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
VAV2 DH/PH/ZF (Vav2 Mouse)

Use

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
NSP2-1x-BioID2 pBabe-puro (SARS-CoV-2)

Plasmid

#185996

Purpose

Expresses SARS-CoV-2 NSP2 fused to BioID2 and HA tag

Depositor

Kyle Roux

Article

May et al Viruses. 2022 Mar 15;14(3). pii: v140306

Insert

SARS-CoV-2 NSP2 (ORF1ab SARS-CoV-2)

Use

Retroviral

Tags

BioID2 and HA

Expression

Mammalian

Mutation

Promoter

LTR

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
1358 pBabe puroL FKHR AAA delta DB

Plasmid

#10697

Depositor

William Sellers

Insert

FKHR AAA deltaDB (FOXO1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

T24A S256A S319A , deletion in aa 208-220

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pBabe 3XFLAG-S37A-GATA6-3XAU1 puro

Plasmid

#72608

Purpose

For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#37 to alanine mutation

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Retroviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Mutation

changed serine37 to alanine

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only