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pCAGGs-NLS-TIR1_P2A_NES-TIR1
Plasmid#117699PurposeExpression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNADepositorInsertsTRANSPORT INHIBITOR RESPONSE 1
TRANSPORT INHIBITOR RESPONSE 1
UseTagsHA tag, Myc-NES, and NLSExpressionMammalianMutationcodon optimized for murine expressionPromoterNone, 2A peptide from porcine teschovirus-1 polyp…Available sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPyPGK-Myc-LaG17-SynNotch-tTA-IRES-Ble
Plasmid#183607PurposeMammalian expression vector: mouse Pgk1 promoter driving expression of a SynNotch receptor comprising anti-GFP nanobody (LaG17), mouse Notch1 core, tTA. Confers bleomycin/zeocin resistance.DepositorInsertLaG17 GFP nanobody SynNotch tTA
UseTagsMyc and human CD8a signal sequenceExpressionMammalianMutationPromoterMouse Pgk1Available sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
mVenusN-Cavin1
Plasmid#108887PurposeExpression of Cavin1 fused to the N-terminus of mVenus for bimolecular fluorescence complementationDepositorInsertcaveolae associated protein 1 (CAVIN1 Synthetic, Human)
UseTagsmVenus1-155ExpressionMammalianMutationaltered codon usagePromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
(318) pcDNA3.1-myc-OGA(1-400)D174N
Plasmid#168096PurposeMyc tagged inactive truncated human OGA catalytic domain of residues 1-400 with D174N mutationDepositorInsertmyc-OGA(1-400, D174N)
UseTagsmycExpressionMammalianMutationD174N catalytically inactive OGAPromoterT7/CMVAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
ALFA-bArr1
Plasmid#201495PurposeExpression of the beta-arrestin1 containing a N-terminal ALFA tag.DepositorInsertALFA-bArr1 (ARRB1 Human)
UseTagsALFAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pBXNPHM3-Nb_MsbA#1
Plasmid#186428PurposePlasmid for bacterial expression of MsbA binding Nanobody Nb_MsbA#1DepositorInsertNanobody Nb_MsbA#1
UseAffinity Reagent/ AntibodyTags3C cleavage site, His Tag, Maltose Binding Protei…ExpressionMutationPromoterpBADAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
mVenusC-Cavin1
Plasmid#108888PurposeExpression of Cavin1 fused to the C-terminus of mVenus for bimolecular fluorescence complementationDepositorInsertCavin1 (altered codon usage) (CAVIN1 Synthetic, Human)
UseTagsmVenus156-239ExpressionMammalianMutationaltered codon usagePromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
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bArr1-ALFA
Plasmid#201496PurposeExpression of the beta-arrestin1 containing a C-terminal ALFA tag.DepositorInsertbArr1-ALFA (ARRB1 Human)
UseTagsALFAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
ALFA-bArr2
Plasmid#201497PurposeExpression of the beta-arrestin2 containing a N-terminal ALFA tag.DepositorInsertALFA-bArr2 (ARRB2 Human)
UseTagsALFAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
bArr2-ALFA
Plasmid#201498PurposeExpression of the beta-arrestin2 containing a N-terminal ALFA tag.DepositorInsertbArr2-ALFA (ARRB2 Human)
UseTagsALFAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBC2T-Ypet-FtnA
Plasmid#108969Purposeexpresses BC2-tag-Ypet-FtnA in mammalian cellsDepositorInsertFtnA (ftnA Escherichia coli)
UseTagsBC2-tag and YpetExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-H2B
Plasmid#108883PurposeMultisite gateway vector for 5' tagging with the human histone H2B sequence (for nuclear localisation)DepositorInsertH2B (human amino acid sequence but different codon usage) (H2BC21 Synthetic, Human)
UseMultisite gatewayTagsExpressionMutationhuman amino acid sequence but different codon usa…PromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-VIM-BC2T
Plasmid#108966Purposeexpresses mCherry-vimentin-BC2-tag in mammalian cellsDepositorInsertVIM (VIM Human)
UseTagsBC2T and mCherryExpressionMammalianMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pABVec-NbH11-D4_huIgG1
Plasmid#160314PurposeExpresses Expresses nanobody H11-D4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2 as a huIgG1 Fc fusionDepositorInsertVHH D4-Fc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pABVec-NbH11-H4_huIgG1
Plasmid#160313PurposeExpresses nanobody H11-H4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2 as a huIgG1 Fc fusionDepositorInsertVHH H4-Fc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6
Plasmid#192789PurposeaGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labelingDepositorInsertantiGFP nanobody minimizer
UseTagsH6 and ybbRExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOD1988-epiDEG
Plasmid#89357PurposeMosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pdpy-7 (epidermis specific).DepositorInsertvhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
UseTargeting vector for mos1 transposon mediated sin…TagsvhhGFP4 (GFP nanobody)ExpressionMutationPromoterPdpy-7Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only
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