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Plasmid#52149PurposeEncodes module 3 with amino acids 12N-16Q for recognition of nt 6UDepositorInsertPumilio homology domain amino acids 97-132 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12C to 12N in Module 3PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-3SR
Plasmid#52150PurposeEncodes module 3 with amino acids 12S-16R for recognition of nt 6CDepositorInsertPumilio homology domain amino acids 97-132 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12C to 12S and 16Q to 16R in Module 3PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-4CQ
Plasmid#52151PurposeEncodes module 4 with amino acids 12C-16Q for recognition of nt 5ADepositorInsertPumilio homology domain amino acids 133-168 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12C in Module 4PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-4SR
Plasmid#52154PurposeEncodes module 4 with amino acids 12S-16R for recognition of nt 5CDepositorInsertPumilio homology domain amino acids 133-168 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12S and 16Q to 16R in Module 4PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-4CYQ
Plasmid#52155PurposeEncodes module 4 with amino acids 12C-13Y-16Q for recognition of nt 5ADepositorInsertPumilio homology domain amino acids 133-168 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12C and 13H to 13Y in Module 4PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-4NYQ
Plasmid#52157PurposeEncodes module 4 with amino acids 12N-13Y-16Q for recognition of nt 5UDepositorInsertPumilio homology domain amino acids 133-168 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 13H to 13Y in Module 4PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-5SE
Plasmid#52160PurposeEncodes module 5 with amino acids 12S-16E for recognition of nt 4GDepositorInsertPumilio homology domain amino acids 169-204 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12C to 12S and 16Q to 16E in Module 5PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-5NQ
Plasmid#52161PurposeEncodes module 5 with amino acids 12N-16Q for recognition of nt 4UDepositorInsertPumilio homology domain amino acids 169-204 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12C to 12N in Module 5PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-6CQ
Plasmid#52163PurposeEncodes module 6 with amino acids 12C-16Q for recognition of nt 3ADepositorInsertPumilio homology domain amino acids 205-240 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12C in Module 6PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-6SR
Plasmid#52166PurposeEncodes module 6 with amino acids 12S-16R for recognition of nt 3CDepositorInsertPumilio homology domain amino acids 205-240 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12S and 16Q to 16R in Module 6PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCas34
Plasmid#82386PurposesgRNA targeting YFP expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pY128
Plasmid#127713PurposeLevel 1 yeast shuttle vector for a transcription unit with A - D scars. Clone in with BsaI. Has a high-copy yeast replicon and KanMX selection.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-CrkII
Plasmid#50024PurposepENTR vector for mouse Crk-IIDepositorInsertCrk-II (Crk Mouse)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceDec. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pACYC Destination
Plasmid#207675PurposeTrio “destination vectors” which harbor an operon for constitutive expression of the repressors for each inducible promoter (CymR, PhlF, and VanR for cumate, DAPG and vanillate, respectively).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterCymRC, PhlF, VanCCAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHYX137
Plasmid#202814PurposeSaccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter PCK1. LEU2 selection.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterScPCK1Available sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHYX138
Plasmid#202815PurposeSaccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter ADH2. URA3 selection.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterScADH2Available sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHYX143
Plasmid#202816PurposeControl plasmid expressing mScarlet-I fluorescent protein.DepositorInsertmScarlet-I
UseTagsExpressionYeastMutationPromoterScPCK1Available sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHYX173
Plasmid#202817PurposePlasmid expressing the TEV protease, codon-optimized for Saccharomyces cerevisiae.DepositorInsertTEV protease
UseTagsExpressionYeastMutationPromoterScPCK1Available sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL_dSSN1_EF1a-tNGFR-2A-Puro
Plasmid#200553PurposeDual barcoding cassettes for SSN-seq; Barcode Set 1DepositorInsertsSSN.guide
SSN.F30
tNGFR
UseLentiviralTagsExpressionMutationPromoterEF1a, Human U6, and Mouse U6Available sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL_dSSN2_EF1a-tNGFR-2A-Puro
Plasmid#201112PurposeDual barcoding cassettes for SSN-seq; Barcode Set 2DepositorInsertsSSN.guide
SSN.F30
tNGFR
UseLentiviralTagsExpressionMutationPromoterEF1a, Human U6, and Mouse U6Available sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only