Addgene

We narrowed to 3,798 results for: SPL

Showing: 621 - 640 of 3798 results

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pCAG-HA-Nrxn1beta AS4(-)

Plasmid

#59409

Purpose

Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]

Depositor

Peter Scheiffele

Article

Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Insert

Nrxn1beta AS4(-) (Nrxn1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-TREtight-mTagBFP2-N2cG

Plasmid

#192838

Purpose

plasmid for making helper virus for monosynaptic tracing using CVS-N2c rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

Depositor

Ian Wickersham

Insert

mTagBFP2 and rabies virus CVS-N2c strain glycoprotein genes (N2cG)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TREtight

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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TR therapy reporter

Plasmid

#179083

Purpose

Fluorescent reporter for alternative splicing

Depositor

Thurman Wheeler

Article

Hu et al Nat Commun. 2018 Dec 7;9(1):5227. doi: 1

Inserts

DsRed1
EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pTrans_9xD3B_Cluc_Norm

Plasmid

#68415

Purpose

CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," transient format.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

Cypridina Luciferase-2A-Venus YFP

Use

Luciferase

Tags

Expression

Mammalian

Mutation

YFP: M153T,V163A,S175G ("Venus") YFP Va…

Promoter

minimalCMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pRegev_V1

Plasmid

#204143

Purpose

Single reporter randomly selected from library for biochemical validation

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

V1 exon in beta globin splicing minigene

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Truncated CAG

Available since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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P16ΔS:GFP1–9

Plasmid

#108186

Purpose

Binary vectors used for the constitutive expression of tripartite split-sfGFP components (GFP1-9) in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Insert

GFP1-9

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

P16ΔS

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti_9xD3B_Cluc_Norm

Plasmid

#68414

Purpose

CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," lentiviral format.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

Cypridina Luciferase-2A-Venus YFP

Use

Lentiviral and Luciferase

Tags

Expression

Mutation

YFP: M153T,V163A,S175G ("Venus") YFP Va…

Promoter

minimalCMV

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pI12

Plasmid

#24273

Depositor

Mariano Garcia-Blanco

Article

Baraniak et al Mol Cell Biol. 2003 Dec . 23(24):9327-37

Insert

modified adenovirus exons and introns

Use

T7 for in vitro transcription

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2010

Availability

Academic Institutions and Nonprofits only

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SPELL-Lyn Component 1

Plasmid

#170484

Purpose

SPELL Lyn kinase (Constitutively active) N-lobe fused to iFKBP. Split site is 279.

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

Lyn(N-lobe), iFKBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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SPELL-Lyn Component 2

Plasmid

#170485

Purpose

SPELL Lyn kinase (Constitutively active) C-lobe fused to FRB. Split site is 279.

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

FRB, Lyn(C-lobe)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pJD427

Plasmid

#154371

Purpose

Expresses FKBP12-XB and SspBcore-FRB from constitutive promoters

Depositor

Joseph Davis

Article

Davis et al ACS Chem Biol. 2011 Nov 18;6(11):1205-13

Insert

proB-RBS(B0032)-SspBcore-FRB-terminator(B0011)-proC-RBS(B0032)-FKBP12-sspBXB-terminator(B0011)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHYRSF236

Plasmid

#46377

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

NpuDnaE intein delta variant, inactive

Use

Tags

H6-Smt3

Expression

Bacterial

Mutation

C1A, C+1A, deleted T76, V97, D98, L100 from NpuDn…

Promoter

T7

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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psc2_md1

Plasmid

#61093

Purpose

splicing reporter construct containing MAG mini-gene

Depositor

Sean Ryder

Article

Zearfoss et al RNA. 2013 Jul;19(7):948-57. doi: 10.1261

Insert

Mag genomic sequence (Mag Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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P16ΔS:(MCS)-S11

Plasmid

#108184

Purpose

Binary vectors used for the constitutive expression of tripartite split-sfGFP components in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

P16ΔS

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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P16ΔS:S10-(MCS)

Plasmid

#108182

Purpose

Binary vectors used for the constitutive expression of tripartite split-sfGFP components in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

P16ΔS

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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TAL3233

Plasmid

#42696

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-crispld2-right (crispld2 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2013

Availability

Academic Institutions and Nonprofits only
TAL3232

Plasmid

#42695

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-crispld2-left (crispld2 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
pMS161

Plasmid

#215683

Purpose

Guide only plasmid targeting chrIII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

U6p::GTCCAGCGGCAGATCGGCGG

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMS160

Plasmid

#215682

Purpose

Guide only plasmid targeting chrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

U6p::GTTTGAGTAGAGCACTCAGG

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRegev_S1.46G>U

Plasmid

#204160

Purpose

pRegev_S1 with single mutation that disrupts secondary structure.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

S1 exon with 46G>U substitution in beta globin splicing minigene

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

46G>U substitution

Promoter

Truncated CAG

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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