Addgene

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pEGFP-PTEN

Plasmid

#110181

Purpose

Expression of PTEN tagged with EGFP

Depositor

Jose Esteban

Article

Jurado et al EMBO J. 2010 Aug 18;29(16):2827-40. doi:

Insert

PTEN (Pten Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAS_8xMmaPylT_EF1_FLAG-MmaPylRS

Plasmid

#174890

Purpose

Mma PylRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

van Husen et al Chembiochem. 2021 Aug 25. doi: 10.1002/c

Insert

Mma PylRS (pylS Methanosarcina mazei)

Use

Tags

FLAG

Expression

Mammalian

Mutation

U25C PylT

Promoter

EF1

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-H2A

Plasmid

#36207

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

H2A (H2AC16 Human)

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-Tubulin

Plasmid

#36202

Purpose

In vivo visualization of microtubules (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

Alpha-tubulin (TUBA1B Human)

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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MSCV-FOXP3-IRES-Thy1.1

Plasmid

#17443

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Insert

FOXP3 (FOXP3 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pPB CAG Otx2-IRES-EGFP

Plasmid

#153947

Purpose

piggyBac transposon vector with CAG promoter expressing Otx2 and EGFP

Depositor

Luca Tiberi

Article

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

Otx2 (Otx2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCRISPR-HOT-Clover-BlastR

Plasmid

#138569

Purpose

Universal Cleavable Donor Plasmid for tagging with Clover-BlastR using NHEJ

Depositor

Hans Clevers

Article

Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

Insert

Clover-BlastR

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pPAICS-2XStrep

Plasmid

#125683

Purpose

expresses human PAICS protein with a C-terminal 2XStrep affinity tag

Depositor

Stephen Benkovic

Article

Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

Insert

PAICS (PAICS Human)

Use

Tags

2X Strep-Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pET27b-ecDHFR-N23PP/L28F

Plasmid

#118584

Purpose

Bacterial expression of E.coli DHFR N23PP and L28F mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Use

Tags

Expression

Bacterial

Mutation

L28 mutated to F28, N23 mutated to P23, a proline…

Promoter

T7

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-ITGA6-1k-Basic-LUC

Plasmid

#107141

Purpose

Luciferase reporter plasmid to monitor transcriptional activity of canine ITGA6-promoter (1kb fragment)

Depositor

Aki Manninen

Article

Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

Insert

ITGA6-promoter

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pRV FOXP3 WWRR

Plasmid

#13625

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Insert

FOXP3 (FOXP3 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

WWRR: T359W, N361W, E399R, E401R

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
LPCX BMP-RIB Q203D

Plasmid

#12642

Depositor

Rik Derynck

Article

Skillington et al J Cell Biol. 2002 Oct 14. 159(1):135-46.

Insert

BMP-RIB (Bmpr1b Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Q203D, activating mutation

Promoter

Available since

Oct. 17, 2006

Availability

Academic Institutions and Nonprofits only
pRV FOXP3 WRR

Plasmid

#13624

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Insert

FOXP3 (FOXP3 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

WRR: N361W, E399R, E401R

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pCK389.AAV

Plasmid

#177327

Purpose

aTc inducible MCP-SoxS double mutant expression for CRISPRa in E.coli. Contains constitutive dCas9 and scRNA expression

Depositor

James Carothers

Article

Tickman et al Cell Syst. 2021 Nov 17. pii: S2405-4712(

Inserts

SoxS
dCas9

Use

CRISPR

Tags

MCP (MS2 Coat Protein)

Expression

Mutation

R93A,S101A

Promoter

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Keasling-2187

Plasmid

#100167

Purpose

Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

Depositor

Jay Keasling

Article

Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

Insert

MevT and MBIS operon

Use

Tags

Expression

Bacterial

Mutation

Promoter

lacUV5

Available since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p)

Plasmid

#70191

Purpose

Derived from 21 with “ClpX” coding sequence from Genbank U00096.3 substituted for “deGFP” – used as T500 template for 134-137.

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

ClpX (clpX )

Use

Tags

Expression

Mutation

Promoter

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG-HUWE1_R4187C

Plasmid

#187142

Purpose

mammalian cell expression of 3xFLAG-HUWE1 R4187C

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

R4187C

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pBEST-p70a-UTR3-deGFP-6xHis-T500

Plasmid

#92224

Purpose

Expression of deGFP using the p70a promoter and UTR3

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

deGFP

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

p70a

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_YH/GG

Plasmid

#187123

Purpose

mammalian cell expression of FLAG-HUWE1 Y355G/H356G

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

1xFLAG

Expression

Mammalian

Mutation

Y355G/H356G

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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JBEI-3716

Plasmid

#100169

Purpose

Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

Depositor

Jay Keasling

Article

Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

Insert

codon-optimized MK

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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