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Plasmid#124252PurposeIn vitro transcription plasmid for T7 transcription of KRAS WT sequence targeted to the endosome. Linearize with SapI.DepositorInsertMinigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
UseIn vitro transcription templateTagsExpressionMutationPromoterT7Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJV129
Plasmid#124253PurposeIn vitro transcription plasmid for T7 transcription of KRAS G12V sequence targeted to the endosome. Linearize with SapI.DepositorInsertMinigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
UseIn vitro transcription templateTagsExpressionMutationPromoterT7Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMTG8-Amp-FRT
Plasmid#128277PurposeTIP-LITer2.0-KRAS(G12V) gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-TIP-P2A-KRAS(G12V)-P2AGFPDepositorInsertsTetR-LOV-TIP
EGFP
KRAS(G12V)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1-myc-BirA*-NRASG12V
Plasmid#122209PurposeExpresses myc-BirA* fused to NRASG12V in mammalian cellsDepositorInsertNRASG12v (NRAS Human)
UseTagsmyc-BirA*ExpressionMammalianMutationchanged G12 to VPromoterCMVAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-NRAS(G12V)
Plasmid#35632DepositorInsertN-Ras(G12V) (NRAS Human)
UseLentiviralTagsHAExpressionMammalianMutationGlycine 12 to ValinePromoterPGKAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
mycBioID-pBabePuro-myc-BirA*NRASG12V
Plasmid#122212PurposeStably expresses myc-BirA* fused to NRASG12V in mammalian cellsDepositorInsertNRASG12V (NRAS Human)
UseRetroviralTagsmyc-BirA*ExpressionMammalianMutationchanged G12 to VPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
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mEos3.2GG
Plasmid#203756PurposeEncodes a modified version of the membrane anchor of KRas, including the last 16 residues of the C terminus, with mEos3.2 fused to the N terminus. The base pairs encoding the CAAX box for KRas were replaced by the CAAX box from rap1B, resulting in a geranylgeranylation instead of farnesylation. Used as a fluorescent plasma membrane probe.DepositorInsertKRas c-term (KRAS Human)
UseTagsmEos3.2ExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
RCAS-K-ras
Plasmid#11549DepositorInsertv-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (Kras Mouse)
UseRetroviralTagsExpressionMutationG12DPromoterAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PM-InPAkt
Plasmid#181915PurposeIndicator of Phosphoinositides using Akt; targeted to the plasma membrane.DepositorInsertpm-InPAkt
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationContains the following mutations with respect to …PromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rgl2-CAAX-pcw107-V5
Plasmid#64641Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertRgl2 plus C-term KRAS (Kras Mouse)
UseLentiviralTagsV5ExpressionMutationC-term of KrasPromoterPGKAvailable sinceDec. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-GFP
Plasmid#35637DepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSPORT-RREB1
Plasmid#41145DepositorInsertRREB-1 (RREB1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLentiCas9-P2A-BFP
Plasmid#149447PurposeExpresses Cas9 and BFP in a lentiviral vector.DepositorInsertP2A-BFP
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterIn frame with Cas9Available sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-SpG
Plasmid#211549PurposePrime editing in mammalian cellsDepositorInsertPEmax-SpG
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBABE puro K-Ras V12
Plasmid#9052PurposeRetroviral expression of a constitutively active form of K-RasDepositorInsertKRAS (KRAS Human)
UseRetroviralTagsExpressionMammalianMutationG12V. Constitutively active.PromoterAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-9K0Q
Plasmid#221739PurposeStable expression of GFP-9K0Q in mammalian cells to monitor membrane chargeDepositorInsertKRAS (KRAS Human)
UseRetroviralTagsGFPExpressionMammalianMutationdeleted amino acids 1-170PromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-SpG-P2A-hMLH1dn
Plasmid#211550PurposePrime editing in mammalian cellsDepositorInsertPEmax-SpG-P2A-hMLH1dn
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDest-635
Plasmid#159582PurposeGateway Destination vector for protein expression in baculovirusDepositorTypeEmpty backboneUseTagsHis6ExpressionInsectMutationPromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only