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Plasmid#125243PurposeExpresses red fluorescent calcium sensor in hippocampal slicesDepositorInsertfRGECO1
UseTagsExpressionMammalianMutationD323APromotersynapsinAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pROSA26-G19
Plasmid#21711DepositorInsertROSA26 G19 (Gt(ROSA)26Sor Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
JPF0522
Plasmid#124013PurposeEncodes crippled pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_pEF1ac-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL T157I-pBabe-puro
Plasmid#19240DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationchanged Threonin 157 to IsoleucinPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL A149T-pBabe-puro
Plasmid#19242DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationchanged Alanin 149 to ThreoninPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
hSyn-PDGFR-KA2-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP
Plasmid#210509Purposeexpresses PDGFR-KA2-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP component in rat hippocampal neuron cellsDepositorInsertsUseTagsMycExpressionMammalianMutationPromoterhSynAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFH2.17_GagZip-MCP-dpol-P2A-eGFP
Plasmid#205532PurposeExpress HIV GagZip-MCP-dpol-P2A-eGFPDepositorInsertGag-MCP-dpol
UseTagsExpressionMammalianMutationNucleocapsid replaced with leucine zipper, pol frā¦PromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
MPRA Empty Vector
Plasmid#204865PurposeVector used for high-throughput cloning and subsequent transfection of MPRA libraries into mammalian cells.DepositorTypeEmpty backboneUseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-LZ_CydAB-mNeongreen
Plasmid#182393PurposeBacterial expression of Vesicle Nucleating peptide-Leucine Zipper and cydAB--mNeongreen fusionsDepositorInsertsVNp-LZ
CydAB-mNeongreen
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRBad-Z-C-DiB-RM
Plasmid#168477PurposeC-fragment of the DiB-RMāsplitāZip protein. This plasmid needs to be co-transformed with Plasmid 168475: pET11a-Z-N-DiB-RM to obtain DiB-RMāsplit-Zip proteinDepositorInsertLeucine Zipper + C-fragment of DiB-RM
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET11a-Z-N-DiB-RM
Plasmid#168475PurposeN-fragment of the DiB-RMāsplitāZip protein. This plasmid needs to be co-transformed with Plasmid 168477: pMRBad-Z-C-DiB-RM to obtain DiB-RMāsplit-Zip proteinDepositorInsertN-fragment of DiB-RM + Leucine Zipper
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
INADL_PDZ_5
Plasmid#103905PurposeProtein expression and purification of INADL PDZ5 domainDepositorInsertINADL (PATJ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
INADL_PDZ_6
Plasmid#103906PurposeProtein expression and purification of INADL PDZ6 domainDepositorInsertINADL (PATJ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
INADL_PDZ_7
Plasmid#103907PurposeProtein expression and purification of INADL PDZ7 domainDepositorInsertINADL (PATJ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
INADL_PDZ_2
Plasmid#103902PurposeProtein expression and purification of INADL PDZ2 domainDepositorInsertINADL (PATJ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
INADL_PDZ_3
Plasmid#103903PurposeProtein expression and purification of INADL PDZ3 domainDepositorInsertINADL (PATJ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
INADL_PDZ_4
Plasmid#103904PurposeProtein expression and purification of INADL PDZ4 domainDepositorInsertINADL (PATJ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
ZAK (ZAKA-c057)
Plasmid#98225PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertZAKA (MAP3K20 Human)
UseBaculovirusTagsHis-6-TEVExpressionMutationpfam00069:Pkinase 16-257PromoterpolyhedrinAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry_C1 I3
Plasmid#61398PurposeExpresses mCherry-tagged Inhibitor-3 in mammalian cellsDepositorInsertInhibitor-3 (PPP1R11 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-GFP-I3
Plasmid#61401PurposeExpresses GFP-tagged Inhibitor-3 in mammalian cellsDepositorInsertInhibitor-3 (PPP1R11 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
XE156 delta LZ-XDpr-CS2+
Plasmid#16766DepositorInsertdelta LZ-XDpr (dact1-b Frog)
UseXenopus expressionTagsExpressionMutationdeletion of first 129 amino acids, including the ā¦PromoterAvailable sinceMarch 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLX304-VHL-IRES-Tdtomato
Plasmid#187643PurposeVHL Expression vector with TdtomatoDepositorInsertVHL (VHL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5 alpha6
Plasmid#16036DepositorInsertalpha6 (ITGA6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLX304-VHL-IRES-GFP
Plasmid#187641PurposeVHL Expression vector with GFPDepositorInsertVHL (VHL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZS*12-MS2-GFP
Plasmid#26245DepositorInsertMS2-GFP
UseTagsExpressionBacterialMutationfive amino acid linker (Ser, Gly, Gly, Gly, Gly) ā¦PromoterAvailable sinceNov. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRK5 c-Fyn
Plasmid#16032DepositorInsertc-Fyn (FYN Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-hs-ALPS 1-ALPS2-mCherry
Plasmid#187114PurposeProtein ExpressionDepositorInsertENSG00000101199 (ARFGAP1 Human)
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5 DN-Fyn
Plasmid#16033DepositorInsertc-Fyn (FYN Human)
UseTagsExpressionMammalianMutationDominant negative: K299MPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
MPRA_minCMV+GFP
Plasmid#204866PurposeVector containing GFP driven by minimal CMV promoter. This cassette can be cloned into MPRA vectors to serve as a reporter gene.DepositorInsertminCMV:GFP
UseTagsExpressionBacterial and MammalianMutationPromoterminimal CMVAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5 beta4
Plasmid#16037DepositorInsertbeta4 (ITGB4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
eGFPbait-E2A-KalTA4-pA donor vector
Plasmid#61069Purposefor CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in combination with a eGFP specific sgRNA (e.g. Plasmid 61051)DepositorInsertseGFPbait
KalTA4
UseZebrafish expressionTagsE2AExpressionMutationPromoterAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 MYC dLZ
Plasmid#176048PurposeExpresses Myc Mutant (delta leucine zipper) in Mammalian cellsDepositorInsertMyc
UseTagsExpressionMammalianMutationdeleted amino acids 414-433PromoterCMVAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Rec-PhoA/CsgA
Plasmid#170787PurposeRecombinase controlled expression of csgA and phoA. Expression is activated upon flipping of inverted proD with corresponding recombinaseDepositorUseSynthetic BiologyTagsHHHHHHExpressionBacterialMutationPromoterInverted proD with Bxb1recognition sites and Inveā¦Available sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-hs-ALPS 1-ALPS2-EGFP
Plasmid#187113PurposeProtein ExpressionDepositorInsertENSG00000101199 (ARFGAP1 Human)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mCherry2-L
Plasmid#104003PurposePlasmid encoding mCherry2-LDepositorInsertmCherry2-L
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (MSKGEE NNLA IIKEFā¦ Vā¦PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAV1-K*R-mEGFP
Plasmid#27766DepositorInsertcaveolin-1, lysine null mutant (CAV1 Human)
UseTagsmonomeric EGFPExpressionMammalianMutationAll lysines mutated to arginines (K*R)PromoterAvailable sinceAug. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mCherry-L
Plasmid#104002PurposePlasmid encoding mCherry-LDepositorInsertmCherry-L
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (MSKGEE DNLA IIKEFā¦ Vā¦PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 BATF3
Plasmid#67050PurposeStandard for cell-free expression benchmarking.DepositorInsertBATF3 (BATF3 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQCXIpuro-H3.3K4M-FLAG/HA
Plasmid#128742PurposeExpression of histone H3.3K4M-FLAG/HADepositorInsertH3.3K4M (H3-3A Human)
UseRetroviralTagsFLAG and HAExpressionMutationK4MPromoterCMVAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNAintron-CDC50A-c3xFLAG
Plasmid#215702PurposeProduces human CDC50A-c3xFLAG, a chaperone to phosphatidylserine flippase complexesDepositorInsertCDC50A (TMEM30A Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-IFT57
Plasmid#218728PurposeExpresses N-terminally EGFP-tagged IFT57 in mammalian cellsDepositorInsertIFT57 (IFT57 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX307-VHL(C162F)-3F-BirA
Plasmid#220149PurposeExpresses VHL(C162F)-BirA as a control for VHL substrate identification by E-STUBDepositorInsertvon Hippel-Lindau tumor suppressor (VHL Human)
UseLentiviralTags3F-BirAExpressionMammalianMutationchanged cysteine 162 to phenylalanine; codon optiā¦PromoterEF-1aAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX307-VHL(Y98H)-3F-BirA
Plasmid#220148PurposeExpresses VHL(Y98H)-BirA as a control for VHL substrate identification by E-STUBDepositorInsertvon Hippel-Lindau tumor suppressor (VHL Human)
UseLentiviralTags3F-BirAExpressionMammalianMutationchanged tyrosine 98 to histidine; codon optimizedPromoterEF-1aAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-Alox5-PLAT-EGFP-6H
Plasmid#187189PurposeProtein ExpressionDepositorInsertENSDARG00000057273 (alox5a Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-hs-AKT1-PH-EGFP-6H
Plasmid#187157PurposeProtein ExpressionDepositorInsertENSG00000142208 (AKT1 Human)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pmCherry_C1 I3(resist)
Plasmid#61399PurposeExpresses mCherry tagged Inhibitor-3 resistant to I3 S2 siRNA in mammalian cellsDepositorInsertInhibitor-3 (PPP1R11 Human)
UseTagsmCherryExpressionMammalianMutationsilent mutations of bases C183T T192C T189CPromoterCMVAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-GFP-I3(resist)
Plasmid#61400PurposeExpresses GFP-tagged Inhibitor-3 resistant to I3 S2 siRNA in mammalian cellsDepositorInsertInhibitor-3 (PPP1R11 Human)
UseTagsGFPExpressionMammalianMutationsilent mutations of bases C183T T192C T189CPromoterCMVAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-GFP-I3(V41A/W43A)
Plasmid#61402PurposeExpresses GFP-tagged, PP1 binding-deficient Inhibitor-3 in mammalian cellsDepositorInsertinhibitor-3 (PPP1R11 Human)
UseTagsGFPExpressionMammalianMutationV41A/W43APromoterCMVAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -