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Showing: 1551 - 1600 of 2028 results

  6. pcDNA5-EGFP-NLS-T2A-mCherry-PTS1

    Plasmid
    #87827
    Purpose
    Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.
    Depositor
    Andrea Musacchio
    Article
    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1
    Inserts
    NLS
    PTS1
    Use
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter, tetracycline operator
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    87827-plasmid-map-sequence-id-175008

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  7. qTAG-C-mScarlet-Puro-H2BC11

    Plasmid
    #207761
    Purpose
    Donor template for mScarlet-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a mScarlet-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 17, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207761-plasmid-map-sequence-id-415325

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  8. pFastBacDual with LFn + Fla

    Plasmid
    #84866
    Purpose
    Baculovirus Expression of LFn -Fla fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein
    Depositor
    Russell Vance
    Article
    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi
    Inserts
    LFn
    Fla
    Use
    Baculovirus
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    84866-plasmid-map-sequence-id-162891

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  9. qTAG-C-moxGFP-Blast-H2BC11

    Plasmid
    #207757
    Purpose
    Donor template for moxGFP-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a moxGFP-Blast Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 16, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207757-plasmid-map-sequence-id-415158

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  10. qTAG-C-mNeon-Blast-TUBB4B

    Plasmid
    #207786
    Purpose
    Donor template for mNeon-2A-Blast insertion into the C-terminus of the TUBB4B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBB4B Addgene #207785
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBB4B Homology Arms flanking a mNeon-Blast Cassette (TUBB4B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    207786-plasmid-map-sequence-id-413796

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  14. pAAV-SynI-CreOn-FlpOff-MCS-P2A-EGFP

    Plasmid
    #176276
    Purpose
    Viral vector for co-expression of a CDS of interest and EGFP in cells expressing Cre AND NOT Flp driven by a Synapsin promoter. Contains an in-frame P2A sequence and an MCS for cloning of the CDS.
    Depositor
    Martin Riccomagno
    Article
    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    MCS-P2A-EGFP
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin I
    Available since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    176276-plasmid-map-sequence-id-350687

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  19. qTAG-C-moxGFP-Puro-H2BC11-MMEJ

    Plasmid
    #207760
    Purpose
    MMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207760-plasmid-map-sequence-id-413789

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  25. pEF1α-M2rtTA-T2A-Puro

    Plasmid
    #169175
    Purpose
    piggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174
    Depositor
    Igor Slukvin
    Article
    Jung et al Cell Rep. 2021 Feb 16;34(7):108758. doi:
    Insert
    M2rtTA-T2A-Puro
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    See Depositor Comments Below
    Promoter
    EF1a
    Available since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    169175-plasmid-map-sequence-id-362474

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  29. PHKG2_HUMAN_D0

    Plasmid
    #79736
    Purpose
    This plasmid encodes the kinase domain of PHKG2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    Nicholas Levinson, John Chodera, Markus Seeliger
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    PHKG2 (PHKG2 Human)
    Use
    Tags
    His10-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  31. pFastBacDual with LFn + PrgJ

    Plasmid
    #84869
    Purpose
    Baculovirus Expression of LFn-PrgJ fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein
    Depositor
    Russell Vance
    Article
    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi
    Inserts
    LFn
    PrgJ
    Use
    Baculovirus
    Tags
    6xHis
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin
    Available since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    84869-plasmid-map-sequence-id-162908

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  32. pMuLE Lenti Dest IFP1.4

    Plasmid
    #62177
    Purpose
    MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses IFP1.4 under the control of a PGK promoter. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    Lentiviral; Mule gateway destination vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62177-plasmid-map-sequence-id-347594

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  33. pMuLE Lenti Dest Luc2

    Plasmid
    #62179
    Purpose
    MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses firefly luciferase under the control of a PGK promoter. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    Luciferase; Mule gateway destination vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62179-plasmid-map-sequence-id-101463

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  35. pY036_ATP1A1_G5

    Plasmid
    #86618
    Purpose
    Expresses the ATP1A1 G5 crRNA in combination with AsCpf1-3xHA to target ATP1A1 intron 4. U6-crRNA(ATP1A1 G5)-CBh-AsCpf1
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G5 crRNA + AsCpf1-3xHA
    Use
    CRISPR; Co-selection via hdr using ouabain
    Tags
    3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86618-plasmid-map-sequence-id-168853

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  39. pBS-AMVpl-β1WT-nAchR

    Plasmid
    #97080
    Purpose
    To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co
    Depositor
    Henry Lester
    Article
    Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.
    Insert
    Beta1 nicotinic acetylcholine receptor (Chrnb1 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    T7
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97080-plasmid-map-sequence-id-189664

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  42. IL1RN gRNA3

    Plasmid
    #113132
    Purpose
    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    IL1RN guideRNA 3 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    Use
    AAV, CRISPR, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    113132-plasmid-map-sequence-id-224944

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  45. qTAG-C-Solo-miRFP670nano3-H2BC11

    Plasmid
    #227331
    Purpose
    Donor template for miRFP670nano3 insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a miRFP670nano3 Tag (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227331-plasmid-map-sequence-id-452128

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  46. qTAG-C-mStayGold-Puro-H2BC11

    Plasmid
    #227332
    Purpose
    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227332-plasmid-map-sequence-id-452132

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  47. qTAG-N-Solo-mStayGold-TUBA1B

    Plasmid
    #227327
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBA1B Homology Arms flanking a mStayGold Tag (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227327-plasmid-map-sequence-id-452117

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  48. qTAG-C-Solo-mStayGold-H2BC11

    Plasmid
    #227330
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a mStayGold Tag (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227330-plasmid-map-sequence-id-452124

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  49. qTAG-C-mStayGold-Puro-PXN

    Plasmid
    #227317
    Purpose
    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    PXN Homology Arms flanking a mStayGold-2A-Puro Cassette (PXN Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227317-plasmid-map-sequence-id-452087

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  50. qTAG-C-mStayGold-EFS-Puro-PLK4

    Plasmid
    #227311
    Purpose
    Donor template for mStayGold-EFS-Puro insertion into the C-terminus of the PLK4 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px458-PLK4 (Addgene #227310)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    PLK4 Homology Arms flanking a mStayGold-EFS-Puro Cassette (PLK4 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227311-plasmid-map-sequence-id-452079

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Showing: 1551 - 1600 of 2028 results