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Plasmid#219551PurposeAkaLuc expression in mammlian cells. pAAV vector. Reporter plasmid.DepositorInsertAkaLuc
UseAAVTagsExpressionMammalianMutationPromoter12xUAS with TATA-box minimal promoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylB_pET29b
Plasmid#215414PurposeExpression construct for NylB gene, codon optimized for expression in E. coliDepositorInsertNylB
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF3362
Plasmid#144838PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMYOG (MYOG Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2B-E2F2
Plasmid#202521PurposeExpressing human E2F2 protein with FLAG-tagDepositorInsertE2F2 (E2F2 Human)
UseTagsFLAG-tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgCrebbp#2/Cre
Plasmid#193210PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Crebbp geneDepositorInsertsgCrebbp#2 (Crebbp Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-MRPS16-VN172
Plasmid#182375PurposemVenus-based mito-riboBiFC vector (Negative control)DepositorInsertMrps16 (Mrps16 Mouse)
UseTagsVN172ExpressionMammalianMutationPromoterCMV promoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
S9_attL1-EGFP-C-term-attL2
Plasmid#128473PurposeEGFP entry cloneDepositorInsertEGFP entry clone
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-Sport6-CD63-pHluorin
Plasmid#130901PurposeExpression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.DepositorInsertCD63-pHluorin (CD63 Human, Aequorea victoria)
UseTagspHluorinExpressionMammalianMutationpHluorin inserted between Gln-36 and Leu-37 in ex…PromoterCMVAvailable sinceOct. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry2-N1
Plasmid#54517PurposeLocalization: N1 Cloning Vector, Excitation: 587, Emission: 610DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsmCherry2ExpressionMammalianMutationPromoterCMVAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-GFP
Plasmid#58977Purposeexpression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA repliconDepositorInsertEGFP
UseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoter26S subgenomic promoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FAM134C-GFP
Plasmid#201927PurposeFor lentiviral transduction of FAM134C-GFPDepositorInsertFAM134C (RETREG3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-pHluorin_M153R-CD63
Plasmid#172117PurposeFluorescent reporter for CD63-positive exosome secretionDepositorInsertCD63 (CD63 Human)
UseLentiviralTagspHluorin_M153R in a small extracelluar loop of CD…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCas9_GFP
Plasmid#44719PurposeCo-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cellsDepositorInsertCas9-2A-GFP
UseCRISPRTags2A-GFPExpressionMammalianMutationPromoterCAGAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
EGFP-pBAD
Plasmid#54762PurposeLocalization: Protein Expression Vector , Excitation: 488, Emission: 507DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTags6xHis and EGFPExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
Str-Ii_SBP-mCherry-Golgin84
Plasmid#65304Purposesynchronize trafficking of Golgin-84 from the ER (RUSH system)DepositorInsertGolgin-84 (GOLGA5 Human)
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-mROCK2
Plasmid#101296PurposeMammalian expression of Rock2-GFPDepositorInsertRock2 (Rock2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Bla
Plasmid#60526PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: blasticidin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Mfn2-HA
Plasmid#139192PurposeExpresses C-terminal HA tagged wt human Mfn2 in mammalian cellsDepositorInsertMFN2 (MFN2 Human)
UseTags3HAExpressionMammalianMutationPromoterCMV promoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pET24a-ncOGT-FL
Plasmid#190821PurposeFor expression of full-length human ncOGT in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.DepositorInsertO-GlcNAc Transferase (OGT Human)
UseTags6x HisExpressionBacterialMutationCodon optimized for expression in E.coliPromoterT7Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pC13N-dCas9-BFP-KRAB
Plasmid#127968Purposeconstitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)DepositorInsertCLYBL-CAG-dCas9-NLS-BFP-KRAB
UseTALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNAintron-XKR8-n3xFLAG
Plasmid#215693PurposeProduces the XKR8 lipid scramblase with an n-terminal 3xFLAG tagDepositorInsertXKR8-n3xFLAG (XKR8 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-myc-OGA(GS-544)
Plasmid#193995Purposeplasmid encoding myc-tagged OGA(GS-544)DepositorInsertmyc-OGA(GS-544)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
BCL-XL-pcw107-V5
Plasmid#64631Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertBCL2L1 (transcript variant 1) (BCL2L1 Human)
UseLentiviralTagsV5ExpressionMutationPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
p6XHis-T7(P266L)
Plasmid#174866PurposeExpresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.DepositorInsertT7 RNA polymerase
UseTags6XHis-tagExpressionBacterialMutationP266LPromoterlac promoterAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXA2
Plasmid#153110PurposeExpression of Flag tagged human FOXA2 in mammalian cellsDepositorInserthuman FOXA2 (FOXA2 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE
Plasmid#207664PurposeNeuropeptide degradationDepositorInsertNEP (MME Human)
UseAAV and Cre/LoxTagsmRUBY3ExpressionMutationAddition of signaling peptide (POMC) on the N-ter…PromoterhSynapsinAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn WT
Plasmid#36046DepositorInsertalpha-synuclein (SNCA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide Puro-P2A-EGFP
Plasmid#137729PurposeFor simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).DepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsEGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only