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Plasmid#101042PurposeAnti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.DepositorInsertAcrIIA2
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265G2
Plasmid#164723PurposeGateway entry clone (attL1 & attR5) A3A/Y130F-iSpyMacCas9-CBE_V01 for C-T base editingDepositorInsertA3A(Y130F)-ziSpyMac(D10A)-UGI)
UseCRISPR; Gateway compatible a3a/y130f-ispymaccas9-…TagsExpressionPlantMutationPromoterAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-CASANOVA
Plasmid#113035PurposeCASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9DepositorInsertCASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCri-H6-TEV-GRAB
Plasmid#128498PurposeExpresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV.DepositorInsertGRAB (Streptococcus pyogenes serotype M1) (grab Streptococcus pyogenes serotype M1)
UseTagsHis tag (6x)ExpressionBacterialMutationPromoterT7Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCri-H6-TEV-GRAB-STREP
Plasmid#128499PurposeExpresses GRAB (from V34 to N181) in bacterial cells. With N-t H6x tag + TEV + protein + STREP(2x) tag.DepositorInsertGRAB (V34-N181) (grab Streptococcus pyogenes serotype M1)
UseTagsHis tag (6x) and Strep tag (2x)ExpressionBacterialMutationPromoterT7Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-CASANOVA
Plasmid#113036PurposeAAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9DepositorInsertCASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTT3-ecdICAM1-ST3-His
Plasmid#210666PurposeExpression of the extracellular domain of human ICAM1 fused to Spytag003 + Histag in HEK293 (or similar)DepositorInsertExtracellular domain of human ICAM-1 fused to Spytag003 and Histag (ICAM1 Human, Synthetic)
UseTagsHistag and Spytag003ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTT3-ecdCD86-ST3-His
Plasmid#210664PurposeExpression of the extracellular domain of human CD86 fused to Spytag003 + Histag in HEK293 (or similar)DepositorInsertExtracellular domain of human CD86 fused to Spytag003 and Histag (CD86 Human)
UseTagsHistag and Spytag003ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTT3-ecdCD80-ST3-His
Plasmid#210665PurposeExpression of the extracellular domain of human CD80 fused to Spytag003 + Histag in HEK293 (or similar)DepositorInsertExtracellular domain of human CD80 fused to Spytag003 and Histag (CD80 Human)
UseTagsHistag and Spytag003ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR1002
Plasmid#71820PurposeExpress Streptococcus pyogenes Cas9DepositorInsertT7pr_10xHis-MBP-TEV-SPyCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only