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Addgene
Showing: 6361 - 6380 of 16015 results
  1. pDmelOR-mRho.V5.mER.hOr47a

    Plasmid
    #126478
    Purpose
    Human codon-optimized D. melanogaster Orco (hOrco) and Or47a (hOr47a). Or47a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells
    Depositor
    Inserts
    Or47a (Or47a Fly)
    Orco (Orco Fly)
    Use
    Tags
    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens and codon optim…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  2. FLAG-Ex1-17-deltaP1P2-PTPN22

    Plasmid
    #195391
    Purpose
    Mammalian expression of a human PTPN22 mutant (exon 1-17 without the P1 and P2 domains) with FLAG tag
    Depositor
    Insert
    PTPN22 (PTPN22 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    PTPN22 mutant containing exon 1 to 17 and lacking…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pTwist-ENTR-MCP-ADAR2-DD(E488Q/T490A)-V5

    Plasmid
    #192713
    Purpose
    Gateway entry vector containing MS2 coat protein fused to ADAR2 catalytic domain (E488Q/T490A) with V5 tag
    Depositor
    Insert
    MCP-ADAR2-DD(E488Q/T490A)-V5
    Use
    Gateway entry vector
    Tags
    V5
    Expression
    Mutation
    E488Q/T490A
    Promoter
    None
    Availability
    Academic Institutions and Nonprofits only
  4. pET28a-mOct4 Full length(FL)

    Plasmid
    #206394
    Purpose
    Expresses Oct4 with a 6xHis tag for protein purification
    Depositor
    Insert
    Oct4 (Pou5f1 Mouse)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. pTH849-gst-optec

    Plasmid
    #89590
    Purpose
    Plasmid for bacterial expression of His-tagged GST encoded by preferred codons
    Insert
    glutathione-S transferase codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  6. pT7-V5-SBP-C1-HshnRNPC1

    Plasmid
    #64920
    Purpose
    Expresses V5- and SBP-tagged hnRNPC1 in mammalian cells
    Depositor
    Insert
    hnRNPC1 (HNRNPC Human)
    Use
    Tags
    V5 and SBP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  7. pT7-V5-SBP-C1-HshnRNPK

    Plasmid
    #64923
    Purpose
    Expresses V5- and SBP-tagged hnRNPK in mammalian cells
    Depositor
    Insert
    hnRNPK (HNRNPK Human)
    Use
    Tags
    V5 and SBP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  8. pJK642

    Plasmid
    #72391
    Purpose
    Produces Escherichia coli glutamine synthetase, N-terminal His6 tag and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F)
    Depositor
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  9. pJK540

    Plasmid
    #72446
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein with N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK)
    Depositor
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7 and n/a
    Availability
    Academic Institutions and Nonprofits only
  10. pLVX-EGFP-C1-centrin-1

    Plasmid
    #73331
    Purpose
    Lentiviral vector encoding centrin-1 with an N-terminal EGFP tag
    Depositor
    Insert
    centrin-1 (CETN1 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  11. pET-HIS-Sangamo

    Plasmid
    #40786
    Purpose
    for e. coli expression, Golden Gate Compatible TALEN incorporated with N Terminal 6xHIS Tag, Codon Optimized FokI
    Depositor
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    N Terminal 6xHIS
    Expression
    Bacterial
    Mutation
    +63 C Terminus, Codon Optimized FokI Endonuclease
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
Showing: 6361 - 6380 of 16015 results