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pKSB-AeU6-2gRNA

Plasmid

#183913

Purpose

gRNA-expressing plasmid under Aedres aegypti AAEL017774 U6 promoter

Depositor

Eric Marois

Article

Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

Insert

gRNA scaffold for protospacer cloning into BbsI sites

Use

Tags

Expression

Mutation

Promoter

Aedes aegypti U6 promoter (AAEL017774)

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pKSB-AeU6-3gRNA

Plasmid

#183914

Purpose

gRNA-expressing plasmid under Aedres aegypti AAEL017763 U6 promoter

Depositor

Eric Marois

Article

Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

Insert

gRNA scaffold for protospacer cloning into BbsI sites

Use

Tags

Expression

Mutation

Promoter

Aedes aegypti U6 promoter (AAEL017763)

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-MR1 (M50P)

Plasmid

#178651

Purpose

Mammalian expression of myc-tagged MR1 (M50P)

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

MR1 (MR1 Human)

Use

Tags

Extracellular c-myc epitope ta and Signal peptide…

Expression

Mammalian

Mutation

M50P

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4 MR1 (H83P)

Plasmid

#178652

Purpose

Mammalian expression of myc-tagged MR1 (H83P)

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

MR1 (MR1 Human)

Use

Tags

Extracellular c-myc epitope ta and Signal peptide…

Expression

Mammalian

Mutation

H83P

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4 MR1 (Q153P)

Plasmid

#178653

Purpose

Mammalian expression of myc-tagged MR1 (Q153P)

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

MR1 (MR1 Human)

Use

Tags

Extracellular c-myc epitope ta and Signal peptide…

Expression

Mammalian

Mutation

Q153P

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-42-DExCon-antiGFPnanobody-mCherry-R11a

Plasmid

#179902

Purpose

Donor with homologous arms for Rab11a to knock in DExCon-antiGFPnanobody-mCherry module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11A, member RAS oncogene family (RAB11A Human)

Use

CRISPR; Donor for homologous based knock in

Tags

antiGFPnanobody-mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

TRE3GS

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-50-DExogron-mCherry-R11b

Plasmid

#179904

Purpose

Donor with homologous arms for Rab11b to knock in DExogron (=minilAA7-DExCon) mCherry module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11B, member RAS oncogene family (RAB11B Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

TRE3GS

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pMKRQ_11_DExCon-mNeonGreen-R25

Plasmid

#179896

Purpose

Donor with homologous arms for Rab25 to knock in DExCon-mNeonGreen module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB25, member RAS oncogene family (RAB25 Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

Promoter

TRE3GS

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-22-DExCon-mNeonGreen-R11a

Plasmid

#179897

Purpose

Donor with homologous arms for Rab11a to knock in DExCon-mNeonGreen module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11A, member RAS oncogene family (RAB11A Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

Promoter

TRE3GS

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-23-DExCon-mCherry-R11b

Plasmid

#179898

Purpose

Donor with homologous arms for Rab11b to knock in DExCon-mCherry module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11B, member RAS oncogene family (RAB11B Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

TRE3GS

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-67-DExCon-mNeonGreen-R11b

Plasmid

#179899

Purpose

Donor with homologous arms for Rab11b to knock in DExCon-mNeonGreen module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11B, member RAS oncogene family (RAB11B Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

Promoter

TRE3GS

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-59-DExCon-mTagBFP2-R25

Plasmid

#179900

Purpose

Donor with homologous arms for Rab25 to knock in DExCon-mTagBFP2 module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB25, member RAS oncogene family (RAB25 Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mTagBFP2

Expression

Bacterial and Mammalian

Mutation

Promoter

TRE3GS

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCaf40-A253FY208A_X

Plasmid

#147973

Purpose

Insect Expression of DmCaf40-A253FY208A

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-A253FY208A (Rcd-1 Fly)

Use

Tags

EGFP (A179V)

Expression

Insect

Mutation

Promoter

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET-UTX(420-633)-H6

Plasmid

#183773

Purpose

Express pET-UTX(420-633)-H6 in E.coli. Purified pET-UTX(420-633)-H6 was used for generating antibodies in rabbits.

Depositor

Peter Harte

Article

Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

Insert

UTX

Use

Tags

6 His tag at the C-terminus

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCaf40-F65AC69Y_X

Plasmid

#147993

Purpose

Insect Expression of DmCaf40-F65AC69Y

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-F65AC69Y (Rcd-1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCaf40_25-291_W

Plasmid

#147866

Purpose

Insect Expression of DmCaf40_25-291

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40_25-291 (Rcd-1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLB37_PB_pGK-H2B-mIFP-T2A-rTetR-HDAC4-zeo

Plasmid

#179440

Purpose

Expresses rTetR-HDAC4(human) for gene silencing, integration by Piggybac

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

H2B-mIFP-T2A-rTetR-HDAC4

Use

Tags

Expression

Mammalian

Mutation

Promoter

pGK

Available since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDestTol2CG2-mnx1:ReaChR-citrine

Plasmid

#169046

Purpose

Destination transgenesis vector for expressing ReaChR fused with citrine in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.

Depositor

Paola Arlotta

Article

Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

Insert

ReaChR-Citrine

Use

Tags

Citrine

Expression

Mammalian

Mutation

Promoter

3xMnx1

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pRabies-EBFP-tTAn

Plasmid

#172120

Purpose

Expression of EBFP and tTA-InteinN in Rabies virus infected cells

Depositor

Chun Xu

Article

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

EBFP, tTAn-InteinN

Use

Rabies virus

Tags

Expression

Mutation

Promoter

CMV

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pRabies-EGFP-rtTAn

Plasmid

#172119

Purpose

Expression of EGFP and rtTA-InteinN in Rabies virus infected cells

Depositor

Chun Xu

Article

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

EGFP, rtTAn-InteinN

Use

Rabies virus

Tags

Expression

Mutation

Promoter

CMV

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge