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Plasmid#162699PurposeExpresses pHnCB10 derived carboxysome operon and prkDepositorInsertpHnCB10 derived carboxysome operon, prk
UseTagsExpressionBacterialMutationPromoterPLtet0-1 promoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSR02
Plasmid#69149PurposeRead-out loxP mCherry to GFP switch for integration on CxTi10816, Mos chr IVDepositorInsertsmCherry
eGFP
UseCre/Lox; MossciTagsExpressionWormMutationcodon-optimzed index 1.0Promoterrps-27Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Sig10-Fc-IG
Plasmid#183722PurposeExpresses the fusion protein containing the extracellular part of human Siglec-10 and the Fc region of human IgG1, and GFPDepositorInsertSiglec-10
UseRetroviralTagsFc of human IgG1ExpressionMutationPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMCS.71/2272.Hygro
Plasmid#22731DepositorInsertLox71-FRT-PGK-hygro-FRT-Lox2272
UseCre/LoxTagsExpressionMutationThis is a basal exchange vector for RMCE. The vec…PromoterAvailable sinceDec. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ER-BSCaM2
Plasmid#64855PurposeEndoplasmic reticulum (ER)-targeted version of a high-affinity FRET-based biosensor for monitoring Ca2+/CaM levels; targeted to cytosolic face of ER membrane.DepositorInsertCYP450-BSCaM2
UseTagsCFP, N-terminal 27 amino acids from CYP450, and Y…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PM-CaNAR2
Plasmid#64730PurposePlasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.DepositorInsertLyn-CaNAR2
UseTagsCerulean3, N-terminal targeting sequence from Lyn…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-17
Plasmid#172747PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-17
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc mouse LNX2
Plasmid#37010DepositorInsertLNX2 (Lnx2 Mouse)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMay 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0402
Plasmid#91009PurposeModule A, Promoter: AtUbi10, Gene: AtCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertAtCas9_dead (D10A + H840A)
UseCRISPRTagsExpressionMutationD10A, H840APromoterAtUbi10Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSR29
Plasmid#69255Purposepelt-2::CRE for integration on ttTi14024, Chr XDepositorInsertCre recombinase
UseCre/Lox; MossciTagsExpressionWormMutationcodon-optimzed index 1.0, tbb-2 UTR is in wrong o…Promoterelt-2Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
LZF1604V2: CAG-spine-jRGECO1a-P2A-QuasAr3-dark Citrine
Plasmid#104122PurposesynOptopatch constructDepositorInsertspine-jRGECO1a-P2A-QuasAr-dark Citrine
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
DNA-CARzeta-IRESpuro
Plasmid#89345Purpose2nd generation lentiviral vector which expresses DNA-CAR receptor upstream of a IRES-puro cassetteDepositorInsertDNA-CARzeta
UseLentiviralTagsSNAPf tagExpressionMammalianMutationPromoterAvailable sinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-16-3xFLAG-LaG-2
Plasmid#172765PurposeBacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (3xFLAG linker)DepositorInsertLaG-16-3xFLAG-LaG-2
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ExRai-AKAR(T/A)
Plasmid#118406PurposeNegative-control mutant for ExRai-AKAR biosensor.DepositorInsertExRai-AKAR(T/A)
UseTags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable sinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP Rab11a S25N pcDNA3.1 Zeo (+)
Plasmid#206080Purposedominant-negative Rab11a mutant - S25N mutation causes inhibition of Rab11a-dependent recyclingDepositorInsertRAB11A (RAB11A Human)
UseTagsEGFPExpressionMammalianMutationS25NPromoterCMVAvailable sinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA
Plasmid#112921PurposeGateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant markerDepositorInsertEF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-ERK2 L198A L232A
Plasmid#8978DepositorInsertERK2 (Mapk1 Rat)
UseTagsHAExpressionMammalianMutationL198A, L232APromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pUBC_HA_PP7-mCherry
Plasmid#107605PurposeTransient expression of non-aggregating delta-FG mutant, fused to mCherry in mammalian cells, under a hUBC promoter, HA tagDepositorInsertPP7-mCherry
UseSynthetic BiologyTagsmCherryExpressionMutationPP7: [delta]67_74 (non-aggregating)PromoterhUBCAvailable sinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP Rab11a WT pcDNA3.1 Zeo (+)
Plasmid#206079PurposeRab11a belongs to the Rab family of small GTPases, Rab11 proteins are particularly involved in trafficking through recycling endosomesDepositorInsertRAB11A (RAB11A Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-sapphireAKAR
Plasmid#118466Purposecp-T-sapphire-based single-color PKA biosensor targeted to the plasma membrane.DepositorInsertLyn-sapphireAKAR
UseTagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only