Addgene

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pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro

Plasmid

#89992

Purpose

Donor template for generation of OCT4-T2A-nEmGFP reporter cell lines

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Res. 2017 Aug;23:105-108. doi:

Insert

OCT4-T2A-NLS-EmGFP-P2A-Puro (POU5F1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLEX-MCS-ASC-GFP

Plasmid

#73957

Purpose

Express human ASC with a C-terminal EGFP tag in mammalian cells

Depositor

Christian Stehlik

Article

de Almeida et al Immunity. 2015 Aug 18;43(2):264-76. doi:

Insert

PYD and CARD domain containing, PYCARD (PYCARD Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMGF182

Plasmid

#97006

Purpose

Expresses GFP-CHMP7 in mammalian cells under a crippled promoter

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

CHMP7 (CHMP7 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV delta 5

Available since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pDest27-mBIN1+13+17

Plasmid

#61097

Purpose

Express N-terminal GST tagged-mouse BIN1+13+17 in mammalian cells

Depositor

Robin Shaw

Article

Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

Insert

mouse BIN1 with exon 13 and 17, excluding exon 7, 11, 14-16

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
LIC1B_Caspase-LOVN7C-7

Plasmid

#104629

Purpose

bacterial expression of light-activated caspase-3 with N7C-7 linker variation

Depositor

Jim Wells

Article

Smart et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

Insert

Caspase-3 (CASP3 Human)

Use

Tags

His and LOV2

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4 human alpha5 full

Plasmid

#221396

Purpose

Mammalian expression of human integrin alpha5 full length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin alpha5 full length (ITGA5 Human)

Use

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and St…

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD6

Plasmid

#195844

Purpose

Expresses human FZD6 with an N-terminal HiBiT; contains M345L

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD6 (FZD6 Human)

Use

Tags

HiBiT

Expression

Mammalian

Mutation

M345L

Promoter

CMV

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-hGSDME

Plasmid

#154876

Purpose

Expresses hGSDME in mammalian cells

Depositor

Judy Lieberman

Article

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (GSDME Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCW57.1-FOXM1b

Plasmid

#68811

Purpose

Doxycycline inducible lentiviral vector for FOXM1b expression in mammalian cells

Depositor

Adam Karpf

Article

Barger et al Oncotarget. 2015 Jul 16.

Insert

FOXM1b (FOXM1 Human)

Use

Lentiviral; Doxycycline inducible

Tags

HA

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 iCasper T2A HO1

Plasmid

#64278

Purpose

Expresses infrared fluorescent executioner caspase reporter (iCasper) plus human heme oxygenase 1

Depositor

Xiaokun Shu

Article

To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

Infrared fluorescent executioner caspase reporter (iCasper) T2A heme oxygenase-1: (HMOX1 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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CaV1.3e[8a,11,31b,Δ32,42a]

Plasmid

#49333

Purpose

Repaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

Depositor

Diane Lipscombe

Article

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Use

Tags

Expression

Mammalian

Mutation

Contains exons 8a, 11, 31b and 42a.Deletion of ex…

Promoter

CMV

Available since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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EphA2-YFP

Plasmid

#108852

Purpose

Encodes for human EphA2 fluorescently labeled with eYFP on the C-Terminus via a 15 amino acid (GGS)5 flexible linker

Depositor

Kalina Hristova

Article

Singh et al J Biol Chem. 2015 Nov 6;290(45):27271-9.

Insert

EPHA2 (EPHA2 Human)

Use

Tags

Labeled with eYFP on the C-Terminus via a 15 amin…

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pMGF196

Plasmid

#97005

Purpose

Expresses LEMD2-mCherry in mammalian cells under a crippled promoter

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

LEMD2 (LEMD2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV delta 5

Available since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-CVS-G-WPRE-pGHpA

Plasmid

#67528

Purpose

"Expresses the rabies glycoprotein"

Depositor

Botond Roska

Article

Wertz et al Science. 2015 Jul 3;349(6243):70-4. doi:

Insert

Rabies virus strain CVS-11 glycoprotein (G)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 23, 2015

Availability

Academic Institutions and Nonprofits only

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pcsII-MECL1-flag

Plasmid

#86774

Purpose

C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

immunoproteasome beta subunit 2 (MECL1) (PSMB10 Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

Promoter

unknown

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP VARP

Plasmid

#42312

Depositor

Thierry Galli

Article

Burgo et al EMBO Rep. 2009 Oct;10(10):1117-24. doi:

Insert

VARP (ANKRD27 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only
pGEX6P1-3xMBT

Plasmid

#46987

Purpose

Inducible expression of 3xMBT from L3MBTL1 as a GST fusion protein in E.coli

Depositor

Or Gozani

Article

Moore et al Mol Cell. 2013 May 9;50(3):444-56. doi:

Insert

Triple MBT domain from human L3MBTL1 (L3MBTL1 Human)

Use

Tags

GST and PreScission cleavage site

Expression

Bacterial

Mutation

contains amino acids 190–530 of accession NP_0562…

Promoter

tac

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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HA-PRAS40 WT

Plasmid

#86758

Purpose

N-terminal HA-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

human PRAS40 (AKT1S1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pcsII-pb5-flag

Plasmid

#86769

Purpose

C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

proteasome beta subunit 5 (PSMB5 Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-AKAP95-FRB

Plasmid

#138254

Purpose

Full-length AKAP95 fused to FRB (rapamycin-binding domain). Used in conjunction with pcDNA3-FKBP-ICUE3-NLS to monitor cAMP dynamics within AKAP95 nanodomain.

Depositor

Jin Zhang

Article

Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

Insert

AKAP95-FRB (AKAP8 Human)

Use

Tags

FRB rapamycin binding domain and HA epitope

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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