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pBEL1953

Plasmid

#195694

Purpose

Complementation of MlaF-MlaE-MlaD(F118A/L123A)-MlaC-MlaB

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaFEDCB

Use

Tags

Expression

Bacterial

Mutation

MlaF-MlaE-MlaD(F118A/L123A)-MlaC-MlaB

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1933

Plasmid

#195695

Purpose

Complementation of MlaF-MlaE-MlaD(F118A)-MlaC-MlaB

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaFEDCB

Use

Tags

Expression

Bacterial

Mutation

MlaF-MlaE-MlaD(F118A)-MlaC-MlaB

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1934

Plasmid

#195696

Purpose

Complementation of MlaF-MlaE-MlaD(L123A)-MlaC-MlaB

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaFEDCB

Use

Tags

Expression

Bacterial

Mutation

MlaF-MlaE-MlaD(L123A)-MlaC-MlaB

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2319

Plasmid

#195697

Purpose

Cryo-EM of 6xHis-Foldon-(Gly-Ser-Linker)-MlaC

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

Foldon-MlaC

Use

Tags

Expression

Bacterial

Mutation

6xHis-Foldon-(Gly-Ser-Linker)-MlaC

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2707

Plasmid

#195636

Purpose

Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP-MlaA

Use

Tags

Expression

Bacterial

Mutation

6xHis-TEV-sfGFP-MlaA (227-251)

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2753

Plasmid

#195637

Purpose

Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; I241N)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP-MlaA

Use

Tags

Expression

Bacterial

Mutation

6xHis-TEV-sfGFP-MlaA (227-251; I241N)

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2754

Plasmid

#195638

Purpose

Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; L245N)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP-MlaA

Use

Tags

Expression

Bacterial

Mutation

6xHis-TEV-sfGFP-MlaA (227-251; L245N)

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2755

Plasmid

#195639

Purpose

Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; I248N)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP-MlaA

Use

Tags

Expression

Bacterial

Mutation

6xHis-TEV-sfGFP-MlaA (227-251; I248N)

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2756

Plasmid

#195640

Purpose

Expression/purification of 6xHis-TEV-sfGFP-MlaA(227-251; delta238-251)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP-MlaA

Use

Tags

Expression

Bacterial

Mutation

6xHis-TEV-sfGFP-MlaA(227-251; delta238-251)

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2757

Plasmid

#195641

Purpose

Expression/purification of 6xHis-TEV-sfGFP-MlaA(227-251; delta244-251)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP-MlaA

Use

Tags

Expression

Bacterial

Mutation

6xHis-TEV-sfGFP-MlaA(227-251; delta244-251)

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2766

Plasmid

#196028

Purpose

Complementation of MlaF-MlaE-MlaDdelta141-183-MlaC-MlaB

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaFEDCB

Use

Tags

Expression

Bacterial

Mutation

MlaF-MlaE-MlaDdelta141-183-MlaC-MlaB

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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GFP SMG1 T3550A/S3556A

Plasmid

#199572

Purpose

Expresses mutated SMG1 fused to GFP

Depositor

Michael Kastan

Article

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

SMG1 (SMG1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

T3550A and S3556A

Promoter

CMV

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NvM-Bam_13-36_AG

Plasmid

#148830

Purpose

Bacterial Expression of DmBam_13-36

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_13-36 (bam Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NvHN-DmBam_13-36_AF

Plasmid

#148742

Purpose

Bacterial Expression of DmBam_13-36

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_13-36 (bam Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCaf40-V186E_AG

Plasmid

#148818

Purpose

Insect Expression of DmCaf40-V186E

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmCaf40-V186E (Rcd-1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpH-DmCaf40_25-291_AF

Plasmid

#148734

Purpose

Bacterial Expression of DmCaf40_25-291

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmCaf40_25-291 (Rcd-1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCaf40-dsRNAres_AA

Plasmid

#148288

Purpose

Insect Expression of DmCaf40-dsRNAres

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmCaf40-dsRNAres (Rcd-1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCaf40-dsRNAres_AA

Plasmid

#148290

Purpose

Insect Expression of DmCaf40-dsRNAres

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmCaf40-dsRNAres (Rcd-1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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GLRX-roGFP2 in pT3TS-Dest

Plasmid

#194297

Purpose

In vitro transcription of the redox sensor GLRX-roGFP2 from the T3 promoter. Parton lab clone KRK

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

GLRX-roGFP2

Use

In vitro transcription of mrna

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET PI-SceI C-His K301A

Plasmid

#195611

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K301A active site mutation

Depositor

Frederick Gimble

Article

He et al J Biol Chem. 1998 Feb 20;273(8):4607-15.

Insert

PI-SceI

Use

Tags

Expression

Bacterial

Mutation

A822G, A823C, A824T

Promoter

T7

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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