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Plasmid#148398PurposeInsect Expression of DmNot1_1147-1673DepositorInsertDmNot1_1147-1673 (Not1 Fly)
UseTagsExpressionInsectMutation2 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1_1377-1673_AC
Plasmid#148400PurposeInsect Expression of DmNot1_1377-1673DepositorInsertDmNot1_1377-1673 (Not1 Fly)
UseTagsExpressionInsectMutation1 silent mutation compared to the sequence given …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsNot1iso1_1085-1605_AC
Plasmid#148413PurposeMammalian Expression of HsNot1iso1_1085-1605DepositorInsertHsNot1iso1_1085-1605 (CNOT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsRck_AC
Plasmid#148417PurposeMammalian Expression of HsRckDepositorInsertHsRck (DDX6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsRck_1-295_AC
Plasmid#148418PurposeMammalian Expression of HsRck_1-295DepositorInsertHsRck_1-295 (DDX6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsRCK-E236Q_H
Plasmid#146456PurposeMammalian Expression of HsRck-E236QDepositorInsertHsRck-E236Q (DDX6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rtn2b-RHD272-472-GFP
Plasmid#186606PurposeEncodes human Reticulon 2 isoform B reticulon homology domain aa 272 to 472 fused to GFPDepositorInsertHuman Reticulon 2 isoform B amino acids 272 to 472 (RTN2 Human)
UseTagsmNeonGreenExpressionMammalianMutationMet-Gly-Ser-Lys peptide added at N-terminus for c…PromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rtn2b-RHD272-472-mNeon
Plasmid#186605PurposeEncodes human Reticulon 2 isoform B reticulon homology domain aa 272 to 472 fused to mNeonGreenDepositorInsertHuman Reticulon 2 isoform B amino acids 272 to 472 (RTN2 Human)
UseTagsmNeonGreenExpressionMammalianMutationMet-Gly-Ser-Lys peptide added at N-terminus for c…PromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsRck_296-472_AC
Plasmid#148419PurposeMammalian Expression of HsRck_296-472DepositorInsertHsRck_296-472 (DDX6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hASCL1 gRNA_1-MS2-Puro
Plasmid#192670PurposeLentiviral expression of sgRNA targeting hASCL1 promoter to activate human ASCL1 transcriptionDepositorInsertHuman ASCL1 activating gRNA #1 (ASCL1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hIL1RN gRNA_1-MS2-Puro
Plasmid#192677PurposeLentiviral expression of sgRNA targeting hIL1RN promoter to activate human IL1RN transcriptionDepositorInsertHuman IL1RN activating gRNA #1 (IL1RN Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Immune checkpoint (library 2) Backbone
Plasmid#192363PurposePlasmid vector for in vivo immune checkpoint screening library (for checkpoint library)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
T cell activation (library 1) Backbone
Plasmid#192364PurposePlasmid vector for in vitro Jurkat activation screening (for immune library)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Gly-tRNA-gRNA scaffold for library 2
Plasmid#192365PurposetRNA sequence provider (for checkpoint library)DepositorInsertGly tRNA-gRNA scaffold
UseFor golden gate assemblyTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pro-tRNA-gRNA scaffold for library 2
Plasmid#192366PurposetRNA sequence provider (for checkpoint library)DepositorInsertPro tRNA-gRNA scaffold
UseFor golden gate assemblyTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Gly-tRNA-gRNA scaffold for library 1
Plasmid#192368PurposetRNA sequence provider (for immune library)DepositorInsertGly tRNA-gRNA scaffold
UseFor golden gate assemblyTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pro-tRNA-gRNA scaffold for library 1
Plasmid#192369PurposetRNA sequence provider (for immune library)DepositorInsertPro tRNA-gRNA scaffold
UseFor golden gate assemblyTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
mRFP-PA-GFP-RhoAQ63L
Plasmid#187282PurposeExpress pmRFP-PAGFP-RhoA Q63LDepositorInsertRhoA (RHOA Human)
UseTagsmRFP-PAGFPExpressionMammalianMutationRhoA Q63LPromoterCMV, T7Available sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rock1-GBD (840-110 aa) in pEGFPN1
Plasmid#187279PurposeExpress EGFP-Rock1 GTPase-binding domainDepositorInsertRock1 (Rock1 Mouse)
UseTagsEGFPExpressionMammalianMutationGBD (84-110aa)PromoterCMVAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKSB-AeU6-1gRNA
Plasmid#183912PurposegRNA-expressing plasmid under Aedes aegypti AAEL017702 U6 promoterDepositorInsertgRNA scaffold for protospacer cloning into BbsI sites
UseTagsExpressionMutationPromoterAedes aegypti U6 promoter (AAEL017702)Available sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only