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Plasmid#141339PurposeLentiviral vector for expression of human NHEJ1-WT in mammalian cellsDepositorInsertNHEJ1 (NHEJ1 Human)
UseLentiviralTagsVA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRDV5 containing Sb-loop
Plasmid#132695PurposepRDV5 plasmid containing a loop Sybody for the generation of a qPCR standard curveDepositorInsertSb_loop including 5' and 3' flanking sequences used for ribosome display
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
mKate1.31-pBAD
Plasmid#54827PurposeLocalization: Protein Expression Vector , Excitation: 588, Emission: 635DepositorTypeEmpty backboneUseTags6xHis and mKate1.31ExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-EF1a-DIO-GRAB_g5-HT3.0
Plasmid#208726PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons in the presence of Cre recombinaseDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Calnexin-C-14
Plasmid#54020PurposeLocalization: Endoplasmic Reticulum, Excitation: 487, Emission: 509DepositorInsertCalnexin (CANX Human)
UseTagsmEmeraldExpressionMammalianMutationI474N in CalnexinPromoterCMVAvailable sinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
FLAG-TREX1
Plasmid#27218DepositorInsertTREX1 (TREX1 Human)
UseTagsFLAGExpressionMammalianMutationn/aPromoterAvailable sinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IZ-ANXA7-mRuby3
Plasmid#184893PurposeStable expression of ANXA7-mRuby3 (Annexin A7-mRuby3) in mammalian cellsDepositorInsertANXA7 (ANXA7 Human)
UseTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAM5409
Plasmid#132662PurposeRSF1010-based broad host-range plasmid with RK2 bom site expressing YFP and Sp/Sm resistanceDepositorInsertsaadA
YFP
UseTagsExpressionBacterialMutationPromoterPaadA and PconIIAvailable sinceNov. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC-LYCipi
Plasmid#53279PurposeContains crtE, crtB, crtI, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces lycopene in Escherichia coliDepositorInsertidi
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICE
Plasmid#46960PurposePlasmid for cloning and constitutive or doxycycline-inducible expression of cDNAs. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
Recall-miniCMV-sfGFP
Plasmid#137995PurposeBackbone for generation of ClonMapper Recall PlasmidDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPL5071_TEF1*-Cre_URA3
Plasmid#60930PurposeConstitutive expression vector for Cre-recombinase containing URA3 selection marker.DepositorInsertCre
UseTagsDriven from a mutant TEF1 promoter with 0.16x act…ExpressionYeastMutationPromotermutant TEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC.HA Vav1
Plasmid#14553DepositorInsertVav1 (VAV1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/hChR2-EYFP
Plasmid#20939PurposeMammalian expression of humanized ChR2 fused to EYFPDepositorInsertchannelrhodopsin-2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBPZpGAL4DBDUw
Plasmid#26233DepositorInsertZip-GAL4DBD
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP
Plasmid#45565PurposeExpresses I-Sce I nuclease in mammalian cells and BFP as a trackerDepositorInsertsI-Sce I Nuclease
BFP
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
BPK3082
Plasmid#78742PurposeHuman expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)DepositorInsertLbCpf1 crRNA backbone, without spacer sequence
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAB1677 REPAIR.t3
Plasmid#176320PurposeCMV-HIVNES-GS-dCas13bt3- (GGS)2-huADAR2dd(E488Q) (REPAIR.t3 expression)DepositorInsertsUseCRISPRTagsHIV NESExpressionMammalianMutationE488Q, only the deaminase domain (aa 276-702 are …PromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR
Plasmid#60226PurposeExpresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.DepositorInsertssgRNA
Renilla luciferase
Cre recombinase
UseAAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…TagsCre-HA and Rluc-P2AExpressionMammalianMutationPromoterEFS and U6Available sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG426GPD-ccdB-yeastSMASh-YFP
Plasmid#68854PurposeExpresses SMASh tag fused to YFP at N terminus in yeastDepositorInsertyeastSMASh-YFP
UseTagsSMASh tagExpressionYeastMutationPromoterGPDAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-BAX
Plasmid#16587DepositorInsertBAX alpha (BAX Human)
UseTags2x HAExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBBK28 pHomL-TDH3p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)
Plasmid#179012PurposeParent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK43 pHomL-TDH3p-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179027PurposeParent vector for markerless yeast integration cassettes with untagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK24 pCAS-Tyr-[gRNA: 7=HIS3] (SplitHygR, AmpR)
Plasmid#179008PurposeSp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK04 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitHygR,AmpR)
Plasmid#178988PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
R777-E283 Hs.ROCK1
Plasmid#70567PurposeGateway ORF clone of human ROCK1 [NM_005406.2] with stop codon (for native or N-terminal fusions)DepositorInsertROCK1 (ROCK1 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-hPhyB621-mCherry-HRasCT
Plasmid#100282PurposeExpresses Phytochrome B (1-621 aa) fused with mCherry and Hras C-terminus in mammalian cellsDepositorInsertPhytochrome B (1-621 aa) (PHYB Mustard Weed)
UseTagsmCherry fused with HRas C-terminus for plasma mem…ExpressionMammalianMutationHuman codon optimized PhyB (1-621 aa)PromoterCAG promoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
IBMc396
Plasmid#161954PurposeDemonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHHDepositorInsertpSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST co hPHGDH
Plasmid#154915PurposeExpress codon optimized human PHGDHDepositorInsertPHGDH, codon optimized (PHGDH Synthetic, Human)
UseRetroviralTagsExpressionMammalianMutationCodon optimized for expression in human cellsPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-imp α
Plasmid#119718PurposeExpresses EGFP-tagged human importinα in mammalian cellsDepositorInsertimportinα (KPNA2 Human)
UseTagsEGFPExpressionMammalianMutationcontains amino acids 251-529PromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only