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Plasmid#106157PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6637, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only
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pYPQ138A
Plasmid#69280PurposeGolden Gate entry vector; 8th gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ137A
Plasmid#69279PurposeGolden Gate entry vector;7th gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_R1072A
Plasmid#91859PurposeBacterial expression for Cas13aDepositorInsertLbuCas13a (LbuC2c2) R1072A
UseTagsHis6-MBP-TEV siteExpressionBacterialMutationR1072APromoterAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pM198
Plasmid#173218PurposeExpresses OptGFP(1-9)-GB1DepositorInsertOptGFP(1-9)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJZ002
Plasmid#120231PurposeCRISPR-cas9 targeting in E. coli with ampicillin resistanceDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCas2D
Plasmid#82375PurposedCas9 expressed from aTc-inducible promoter in acsA, D RBS: AGGACGDepositorInsertdCas9
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_K1082A
Plasmid#91863PurposeBacterial expression for Cas13aDepositorInsertLbuCas13a (LbuC2c2) K1082A
UseTagsHis6-MBP-TEV siteExpressionBacterialMutationK1082APromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ZNF503
Plasmid#135735PurposeDonor vector for 3' FLAG tag of human ZNF503DepositorInsertZNF503 Homology arms (ZNF503 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330.iGFP1
Plasmid#66581PurposesgRNA for iGFPDepositorInsertiGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PpU6P-sgRNA-L5L2
Plasmid#113738PurposeGateway entry vector containing attL5 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.DepositorInsertPpU6P::sgRNA
UseCRISPR; Gateway entry vectorTagsExpressionMutationPromoterP. patens U6 promoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330.LoxP
Plasmid#66586PurposesgRNA for LSLDepositorInsertLoxP sgRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKFSS1_Psyn-sgRNA500
Plasmid#149557PurposegRNA expression in B. burgdorferi, parental vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPsyn and pSynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB6371
Plasmid#106153PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6630, integration into Yarrowia lipolytica chromosomal location IntC_3, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ZNF331
Plasmid#135726PurposeDonor vector for 3' FLAG tag of human ZNF331DepositorInsertZNF331 Homology arms (ZNF331 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330.iGFP2
Plasmid#66582PurposesgRNA for iGFPDepositorInsertiGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgRNA_Drosha_4
Plasmid#79863PurposeCRISPR/Cas9 DROSHADepositorInsertsgRNA Drosha
UseCRISPR and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMST665_BB3_L 23_gRNAempty_cas9_BbsI
Plasmid#90278PurposeBB3_L 23_gRNAempty_cas9_BbsI; CRISPR plasmid with empty gRNA spacer to incorporate gRNA, Cas9DepositorInsertCas9
UseA. nigerTagsExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgRNA_Drosha_1
Plasmid#79860PurposeCRISPR/Cas9 DROSHADepositorInsertsgRNA Drosha
UseCRISPR and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgRNA_Drosha_2
Plasmid#79861PurposeCRISPR/Cas9 DROSHADepositorInsertsgRNA Drosha
UseCRISPR and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only