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pTn7CdrA-gfp(ASV)C

Plasmid

#111617

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

Depositor

Tim Tolker-Nielsen

Publication

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(ASV)-T0-T1

Expression

Bacterial

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14

Plasmid

#213518

Purpose

Expresses NSP14 of SARS-CoV-2

Depositor

Thomas Tuschl

Publication

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

N-terminal His6-sumo

Expression

Bacterial

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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BSD-CryC

Plasmid

#209672

Purpose

Expresses CRY2 fused with Split-TurboID-C and mCherry in mammalian cells

Depositor

Sanjeevi Sivasankar

Publication

Shafraz et al J Cell Sci. 2023 Oct 1;136(19):jcs261430

Insert

CRY2 (CRY2 Mustard Weed)

Use

Lentiviral

Tags

Split-TurboID-C and mCherry

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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CibN

Plasmid

#209671

Purpose

Expresses CIB1 fused with Split-TurboID-N and pmGFP in mammalian cells

Depositor

Sanjeevi Sivasankar

Publication

Shafraz et al J Cell Sci. 2023 Oct 1;136(19):jcs261430

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CIB1 (CIB1 Mustard Weed)

Tags

Split-TurboID-N and pmEGFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pMP4518

Plasmid

#107778

Purpose

Expression of EYFP

Depositor

Herman Spaink

Publication

Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

pLacZ-EYFP in pBBR1-mcs5

Tags

pLacZ-EYFP

Expression

Bacterial

Promoter

pLacZ

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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ZNF668-Flag

Plasmid

#42636

Depositor

Shiaw-Yih Lin

Publication

Hu et al Cancer Res. 2011 Oct 15;71(20):6524-34.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ZNF668 (ZNF668 Human)

Tags

3xFLAG

Expression

Mammalian

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only
pDN-G1TGt

Plasmid

#44508

Depositor

Gabor Balazsi

Publication

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
tetR::yEGFP

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Two tandem tet operators (2XtetO2) downstream of …

Promoter

PGal1-D12 promoter

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pDN-T1TCbt

Plasmid

#44514

Depositor

Gabor Balazsi

Publication

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-T123 promoter (GAL1 Budding Yeast, Synthetic)
tetR::ymcherry

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Three tandem tet operators (3XtetO2) downstream o…

Promoter

PGal1-T123 promoter

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pATLIC

Plasmid

#110287

Purpose

A backbone vector for cell surface display in E. coli by Ligation Independent Cloning (LIC)

Depositor

In-Geol Choi

Publication

Ko et al Appl Environ Microbiol. 2012 May;78(9):3

Insert

yfaL

Expression

Bacterial

Promoter

araBAD promoter

Available since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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pcsII-pb2-flag

Plasmid

#86766

Purpose

C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

Depositor

Do-Hyung Kim

Publication

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

proteasome beta subunit 2 (PSMB2 Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Promoter

EF-1a

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDisplay-DNER

Plasmid

#51053

Purpose

expression of HA-DNER in mammalian cells

Depositor

Mineko Kengaku

Publication

Eiraku et al J Biol Chem. 2002 Jul 12;277(28):25400-7

Insert

DNER (Dner Mouse)

Tags

HA and signal peptide

Expression

Mammalian

Mutation

without endogenous signal peptide (1-221bp)

Promoter

CMV

Available since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pLVX-Puro-mGSDME F2A

Plasmid

#154878

Purpose

Expresses mGSDME F2A in mammalian cells

Depositor

Judy Lieberman

Publication

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (Gsdme Mouse)

Use

Lentiviral

Mutation

changes Phenylalanine 2 to Alanine

Promoter

CMV

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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T7-5'UTR-Pa01-bpNLS-3'UTR-polyA (CJT484)

Plasmid

#248218

Purpose

Pa01-bpNLS IVT vector for mRNA production

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

5'UTR-Pa01-bpNLS-3'UTR-polyA

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

None

Promoter

T7

Available since

June 4, 2026

Availability

Academic Institutions and Nonprofits only

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T7-5'UTR-SuperDn29-bpNLS-3'UTR-polyA (CJT611)

Plasmid

#248220

Purpose

bpNLS-SuperDn29 IVT vector for mRNA production

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

5'UTR-SuperDn29-bpNLS-3'UTR-polyA

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

Super Dn29(M6I, E70G, A224P, G227V, I233K, K248R,…

Promoter

T7

Available since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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Dn29 attD pDonor (CJT454)

Plasmid

#248228

Purpose

Dn29 attP pDonor with Nt.BbvcI site for cssDNA production

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

Dn29 attD

Use

CRISPR; In vitro transcription

Expression

Mammalian

Mutation

None

Promoter

NA

Available since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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Pa01 attP60 pDonor (CJT460)

Plasmid

#248225

Purpose

Pa01 attP pDonor with Nt.BbvcI site for cssDNA production

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

Pa01 attP

Use

CRISPR; In vitro transcription

Expression

Mammalian

Mutation

None

Promoter

NA

Available since

May 11, 2026

Availability

Academic Institutions and Nonprofits only

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LTR-[Bxb1(attB)-spacer-BceINT(attB)-spacer-Pa01(attB)-spacer-SacINT(attB)-pEF1a-Puro-LTR (DRR724)

Plasmid

#248231

Purpose

Lentiviral plasmid containing Bxb1, BceINT, Pa01, and SacINT attB target sites

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

LTR-[Bxb1(attB)-spacer-BceINT(attB)-spacer-Pa01(attB)-spacer-SacINT(attB)-pEF1a-Puro-LTR

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

None

Promoter

NA

Available since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-bpNLS-IS621 (CJT489)

Plasmid

#248213

Purpose

bpNLS-tagged IS621 bridge recombinase, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

bpNLS-IS621

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

None

Promoter

CMV and T7

Available since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-SV40NLS-Stab-exin21-Bxb1(A315R)-Stab (CJT604)

Plasmid

#248210

Purpose

SV40 NLS-, stabilon-, and exin21-tagged Bxb1 (A315R) recombinase, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

SV40NLS-Stab-exin21-Bxb1(A315R)-Stab

Use

CRISPR; In vitro transcription

Tags

SV40NLS

Expression

Mammalian

Mutation

Bxb1(A315R)

Promoter

CMV and T7

Available since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-bpNLS-Bxb1 (EMK569)

Plasmid

#248208

Purpose

Bxb1 recombinase with N-terminal bpNLS, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

bpNLS-Bxb1

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

None

Promoter

CMV and T7

Available since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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