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Plasmid#192143PurposeExpresses Nsp2Cas9, and cloning backbone for sgRNADepositorInsertNsp2Cas9
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
NarCas9_AAV
Plasmid#192145PurposeExpresses NarCas9?and cloning backbone for sgRNADepositorInsertNarCas9
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSK-mU6-Ccu-sgRNA-SV40-puro
Plasmid#192130PurposeExpresses Ccu-SgRNA scaffold cloned with BbsI and puromycin resistance geneDepositorInsertCcu-SgRNA scaffold
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSK-mU6-Hsp1-sgRNA-SV40-puro
Plasmid#192129PurposeExpresses Hsp1-SgRNA scaffold cloned with BbsI and puromycin resistance geneDepositorInsertHsp1-SgRNA scaffold
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSK-mU6-Cj-sgRNA-SV40-puro
Plasmid#192128PurposeExpresses Cj-SgRNA scaffold cloned with BbsI and puromycin resistance geneDepositorInsertCj-SgRNA scaffold
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENT-L3-eBra-L4
Plasmid#186368PurposeEntry clone with ORF encoding Brachury cis-regulatory region flanked by Gateway recombination sequencesDepositorInsertBrachury notochord enhancer
UseProtein expressionTagsExpressionMutationPromoterT7 promoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L3-Titf-L4
Plasmid#186367PurposeEntry clone with ORF encoding Titf1 cis-regulatory region flanked by Gateway recombination sequencesDepositorInsertTitf endoderm enhancer
UseProtein expressionTagsExpressionMutationPromoterT7 promoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::RfA
Plasmid#186396PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of a regulatory sequence cloned in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-Venus-stop-L2
Plasmid#186360PurposeEntry clone with ORF encoding Venus fluorescent protein flanked by Gateway recombination sequencesDepositorInsertVenus yellow fluorescent protein
UseExpression of the venus fluorescent proteinTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
DUX4_KBM pDonor221
Plasmid#187802PurposeDUX4 with KBM (p. E414-L424 deletion) entry cloneDepositorInsertDUX4-KBM (DUX4 Human)
UseTagsExpressionMammalianMutationp. E414-L424 deletionPromoterAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-ZmIAA29 (yeast codon optimized)
Plasmid#188441PurposeZea Mays Nuclear Auxin Signaling Repressor IAA29 with yeast codon optimizationDepositorInsertAuxin Signaling Repressor IAA29 (yeast codon optimized)
UseCloningTagsExpressionMutationPromoterAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-NF1-L1-dSpRY
Plasmid#177681PurposeGateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editingDepositorInsertFokI-dCas9-SpRY
UseCRISPR; Gateway compatible foki-dcas9-spry entry …TagsNLSExpressionPlantMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-NF1-L2-dSpRY
Plasmid#177682PurposeGateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editingDepositorInsertFokI-dCas9-SpRY
UseCRISPR; Gateway compatible foki-dcas9-spry entry …TagsNLSExpressionPlantMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-NF-L2-dMb2Cas12a
Plasmid#177684PurposeGateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editingDepositorInsertFokI-dMb2Cas12a
UseCRISPR; Gateway compatible foki-dmb2cas12a entry …TagsNLSExpressionPlantMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pMC8b-XI1up
Plasmid#176166Purposeyeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-1DepositorInsert5' homology for integration site XI-1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC7-XI1dw
Plasmid#176167Purposeyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-1DepositorInsert3' homology for integration site XI-1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC8b-XI2up
Plasmid#176168Purposeyeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-2DepositorInsert5' homology for integration site XI-2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC7-XI2dw
Plasmid#176169Purposeyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-2DepositorInsert3' homology for integration site XI-2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC8b-XII2up
Plasmid#176174Purposeyeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XII-2DepositorInsert5' homology for integration site XII-2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only