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mCherry-C1-ORP5-H478-9A-deltaTMD-FKBP
Plasmid#220075Purposeexpresses a mutant ORP5 that is unable to bind to PI4P with an mCherry fluorophore and an FKBPDepositorInsertOSBPL5 H478/9A (OSBPL5 Human)
UseTagsExpressionMammalianMutationH478/9A, deltaTMDPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-C1-ORP5deltaORDdeltaTMD-FKBP
Plasmid#220074Purposeexpresses ORP5 with the OSBP-related ligand-binding domain and the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.DepositorInsertOSBPL5deltaORDdeltaTMD (OSBPL5 Human)
UseTagsExpressionMammalianMutationdeltaORDdeltaTMDPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTagBFP-C1-FKBP-PIP5K1C-HD-EE
Plasmid#220090PurposeRecruitable PIP5K to make PIP2DepositorInsertPIP5K1C (PIP5K1C Human)
UseTagsTagBFP-FKBP1A(3-108)ExpressionMammalianMutationamino acids 1-635; D101R, R304D, R445E, K446EPromoterCMVAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNeonGreen-N1-Nir1-613-630
Plasmid#220088PurposeN-terminal amphipathic helix of Nir1-LNS2 domain; loses ability to bind PADepositorInsertPITPNM3 (PITPNM3 Human)
UseTagsGGSGG-NeonGreenExpressionMammalianMutationamino acids 613-630PromoterCMVAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNeonGreen-C1-Nir2-LNS2
Plasmid#220086PurposeC-terminal PA binding domain of Nir2DepositorInsertPITPNM1 (PITPNM1 Human)
UseTagsNeonGreen-GGSGGMExpressionMammalianMutationamino acids 896-1216PromoterCMVAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNES-EGFP-C1-flex-PABD-Spo20
Plasmid#220081PurposeSpo20-PABD PA biosensor with a flexible linker to aid in nuclear exportDepositorInsertSpo20 (SPO20 Budding Yeast)
UseTagsX. leavis map2k1.L(32-44)-EGFP-SGGGSGGSExpressionMammalianMutationamino acids 51-91PromoterCMVAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lneg-INTR-GFP
Plasmid#208902Purposeencodes the inotocin receptor (oxytocin/vasopressin-like) from the ant Lasius neglectus, tagged with GFPDepositorInsertINTR
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
rSirt3 shRNA (pLKO.1 vector)
Plasmid#216807PurposeshRNA against rat Sirt3DepositorInsertSirt3 (Sirt3 Rat)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-Topo-kcnk5a
Plasmid#208988PurposeRiboprobe generationDepositorInsertkcnk5a (kcnk5a Zebrafish)
UseCloningTagsExpressionMutationPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnk6
Plasmid#208990PurposeRiboprobe generationDepositorInsertkcnk6 (kcnk6 Zebrafish)
UseCloningTagsExpressionMutationPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-Topo-kcnk9
Plasmid#208992PurposeRiboprobe generationDepositorInsertkcnk9 (kcnk9 Zebrafish)
UseCloningTagsExpressionMutationWithout STOP codonPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnk13a
Plasmid#208996PurposeRiboprobe generationDepositorInsertkcnk13a (kcnk13a Zebrafish)
UseCloningTagsExpressionMutationPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP3C-FR-2Strep
Plasmid#210312PurposeExpresses the C terminal of SARS-CoV-2 NSP3 in mammalian cellsDepositorInsertC terminal of SARS-CoV-2 NSP3 (ORF1ab )
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP3C-EGFP
Plasmid#210318PurposeExpresses the C terminal of SARS-CoV-2 NSP3 fused with EGFP tag in mammalian cellsDepositorInsertC terminal of SARS-CoV-2 NSP3 (ORF1ab )
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 N-2Strep
Plasmid#210376PurposeExpresses SARS-CoV-2 N protein fused with 2Strep in mammalian cellsDepositorInsertSARS-COV-2 N (N SARS-CoV-2)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDN0617 (pDEST-mEGFP C-term, NatR)
Plasmid#210484PurposepDEST vector to tag gene of interest with mEGFP on the C-terminus to perform localization assays in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsmEGFPExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Combined γB2FL-FRET probe
Plasmid#209094PurposemTurquoise2- and mVenus-inserted PcdhgB2 full length (FL)DepositorInsertPcdhgB2 (Pcdhgb2 Mouse)
UseTagsPA tagExpressionMammalianMutationPromoterChicken β-actinAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Combined γB2ΔICD-FRET probe
Plasmid#209093PurposemTurquoise2- and mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)DepositorInsertPcdhgB2 (Pcdhgb2 Mouse)
UseTagsPA tagExpressionMammalianMutationPromoterChicken β-actinAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
REEP6-2Strep
Plasmid#210351PurposeExpresses REEP6 fused with 2Strep in mammalian cellsDepositorInsertREEP6 (REEP6 Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only
