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Plasmid#127437PurposeKeratin labeling with near-infrared fluorescent protein miRFP670nanoDepositorInsertKeratin-miRFP670nano
UseTagsmiRFP670nanoExpressionMammalianMutationKeratin V282L - please see depositor comments bel…PromoterCMVAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-omega
Plasmid#114632PurposeAddgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAPDepositorInsertdCas9-omega
UseCRISPRTagsExpressionBacterialMutationPromoterTet promoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
STAGR_SAMScaffold_mU6
Plasmid#102845PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert SAM_mU6
UsePcr template for stagr insertsTagsExpressionMutationPromotermU6Available sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-sapphireAKAR
Plasmid#118466Purposecp-T-sapphire-based single-color PKA biosensor targeted to the plasma membrane.DepositorInsertLyn-sapphireAKAR
UseTagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDGE337
Plasmid#153246PurposeM4E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/UUG-nLuc-3XFLAG
Plasmid#127302PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from UUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_TOP-NLuc1.1_L5-L4
Plasmid#113867PurposeSingle pathway reporter; wnt promoter-driven expression of intracellular NanoLuc; MultiSite Gateway entry vector.DepositorInsertsTOP (wnt dependent promoter)
NLuc
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-sapphireAKAR(T/A)
Plasmid#118465PurposeNegative-control mutant for sapphireAKAR biosensor.DepositorInsertsapphireAKAR(T/A)
UseTags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MBII85-5'SS mut
Plasmid#67631PurposeMBII85 snoRNA expression cassett, contains snoRNA in natural intron surrounded by natural exons. To improve snoRNA expression efficiency 5' splice site was mutated to consensus sequence.DepositorInsertMBII85 snoRNA
UseTagsExpressionMammalianMutationmutation in 5'ssPromoterAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pALS343 (pGEX-2TP-cTevHis-Gibson/MCS)
Plasmid#139788PurposeVector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHisDepositorTypeEmpty backboneUseTags6xHis (cleavable by TEV protease) and GST (cleava…ExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVenusNfN
Plasmid#128564PurposeProtein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.DepositorInsertVenus
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
TB:7xSMAD_RE:MiniP
Plasmid#133879Purpose7 copies the SMAD DNA binding motifDepositorInsert7 copies the SMAD DNA binding motif
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSetB-KanR-LiLac
Plasmid#184569PurposeFluorescent reporter for lactate (with lifetime and intensity response); for bacterial or in vitro expressionDepositorInsertLiLac fluorescent sensor of lactate
UseTagsHis7ExpressionBacterialMutationPromoterT7Available sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3E-APEX2-P2A-mKate2
Plasmid#67671PurposeMultisite gateway entry clone for adding C-terminal fusions of APEX2-P2A-mKate2DepositorInsertAPEX2-P2A-mKate2
UseGateway multisite 3' entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
STAGR_gRNAScaffold_hH1
Plasmid#102841PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert gRNAScaffold_hH1
UsePcr template for stagr insertsTagsExpressionMutationPromoterhH1Available sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
CFP-FRBx5-His
Plasmid#103784Purposeexpresses His-tagged iPOLYMER component in E. coli for protein purificationDepositorInsertCFP-FRBx5-His
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132A2.0
Plasmid#99887PurposeGolden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU6Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVenusCfC
Plasmid#128567PurposeProtein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.DepositorInsertVenus
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVenusCfN
Plasmid#128566PurposeProtein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.DepositorInsertVenus
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ExRai-AKAR-Kras
Plasmid#118408PurposePlasma membrane-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.DepositorInsertExRai-AKAR-Kras
UseTagsC-terminal targeting sequence from KrasExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only