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pSynapsin_NLS_GFP-P2A_SNAP_GluA2

Plasmid

#170455

Purpose

Expresses SNAP tagged GluA2 in neuronal cells

Depositor

Shigeki Watanabe

Article

Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

Insert

GluA2 (Gria2 Mouse)

Use

Lentiviral

Tags

SNAP

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mCherry-CHMP4B

Plasmid

#116923

Purpose

expresses mCherry-CHMP4B in mammalian cells

Depositor

Sanford Simon

Article

Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

Insert

CHMP4B (CHMP4B Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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EGFR L858R

Plasmid

#11012

Purpose

Retroviral construct for expressing human EGFR with L858R mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR L858R (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

L858R (tumor-derived)

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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GFP-TRIM33

Plasmid

#65398

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

TRIM33 (TRIM33 Human)

Use

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-TRIM28

Plasmid

#65397

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

TRIM28 (TRIM28 Human)

Use

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only
pLentiCas9-T2A-mApple

Plasmid

#193780

Purpose

Tricistronic expression of Cas9-mApple-BSD in mammalian cells

Depositor

James Eshleman

Article

Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

Insert

mApple

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

In frame with Cas9

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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SMARCA2-GFP

Plasmid

#65390

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

SMARCA2 (SMARCA2 Human)

Use

Tags

EmGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-hAXL

Plasmid

#124797

Purpose

Tet-inducible AXL expression in mammalian cells

Depositor

Kenneth Pienta

Article

Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

Insert

AXL (AXL Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE3GS

Available since

June 20, 2019

Availability

Academic Institutions and Nonprofits only

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AbVec2.0-mIgkc

Plasmid

#127157

Purpose

Expression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), kappa constant region

Depositor

Hedda Wardemann

Article

Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

Insert

Igkc (Igkc Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV IE1 gene promoter

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCMVNeoDEST(705-1)-hAXL

Plasmid

#124796

Purpose

Expresses AXL in mammalian cells

Depositor

Kenneth Pienta

Article

Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

Insert

AXL (AXL Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-BICD2

Plasmid

#164626

Purpose

Expresses BICD2 in mammalian cells with a GFP tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

BICD2 (BICD2 Human)

Use

Tags

Cycle 3 GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSynapsin_NLS_GFP_P2A_His6_GluA2

Plasmid

#170453

Purpose

Expresses histidine tagged GluA2 in neuronal cells

Depositor

Shigeki Watanabe

Article

Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

Insert

GluA2 (Gria2 Mouse)

Use

Lentiviral

Tags

Hexa-histidine

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pHIV-EGFP.Flag-EZH2

Plasmid

#220243

Purpose

Expresses wild-type EZH2 for knockout/rescue experiments in mammalian cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 (EZH2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

EF-1α

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-HA-ERK2 R67S

Plasmid

#53201

Purpose

Expresses GST and HA tagged human ERK2 R67S from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

R67S

Promoter

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pOD2044-intDEG

Plasmid

#89366

Purpose

MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pelt-2 (intestine specific).

Depositor

Karen Oegema

Article

Wang et al Development. 2017 Jun 15. pii: dev.15009

Insert

vhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)

Use

Targeting vector for mos1 transposon mediated sin…

Tags

vhhGFP4 (GFP nanobody)

Expression

Mutation

Promoter

Pelt-2

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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phDLX2-N174

Plasmid

#60860

Purpose

2nd generation lentiviral transfer plasmid. Expresses DLX2 under the EF1a promoter

Depositor

Andrew Yoo

Article

Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

Insert

DLX2 (DLX2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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AbVec2.0-mIghg2b

Plasmid

#127155

Purpose

Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2b isotype

Depositor

Hedda Wardemann

Article

Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

Insert

Ighg2b (Ighg2b Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV IE1 gene promoter

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pmCTIP2-N174

Plasmid

#60858

Purpose

Expresses CTIP2 under the EF1a promoter

Depositor

Andrew Yoo

Article

Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

Insert

CTIP2 isoform b (Bcl11b Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pS6-hα2M-H6

Plasmid

#128495

Purpose

Expresses human α2M (from G27 to S1468) in mammalian cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

A2M (G27-S1468) (A2M Human)

Use

Tags

His tag (6x)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pLex 983-Micu2

Plasmid

#50372

Purpose

Expression in mammalian cells

Depositor

Vamsi Mootha

Article

Plovanich et al PLoS One. 2013;8(2):e55785. doi: 10.1371

Insert

MICU2 (MICU2 Human)

Use

Lentivirus

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only