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Plasmid#202055PurposeE. coli expression vector for N-ter GFP11 R9 tagged BFPDepositorInsertBFP
UseTags6xHis and GFP11ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
p15a_AcCas12n
Plasmid#203806Purposep15a-based plasmid containing a rpsL driven AcCas12n cassette and the araBAD-driven lambda-red recombinase cassette. Apramycin resistance.DepositorInsertAcCas12n
UseCRISPRTagsExpressionMutationPromoterrpslAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSGKP_AcsgRNA
Plasmid#203807PurposepSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance.DepositorInsertAcgRNA
UseCRISPRTagsExpressionMutationPromoterJ23119Available sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ATG13 (363-517)-MBP
Plasmid#203561PurposeMammalian expression of ATG13 (363-517) with MBP tagDepositorInsertATG13 (ATG13 Human)
UseTagsMBP-10HisExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
cytoDCIP
Plasmid#193861PurposeEncodes mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.DepositorInsertmCherry-GFP1-10
UseTagsExpressionPlantMutationPromoter35SAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
1BR9-Lifeact
Plasmid#193863PurposeE. coli expression vector for N-ter GFP11 and C-ter R9 tagged lifeact peptide.DepositorInsertLifeact
UseTags6xHis, GFP11, and R9ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG13 (460-517)
Plasmid#203552PurposeMammalian expression of GST tagged ATG13 (460-517)DepositorInsertATG13 (ATG13 Human)
UseTagsGSTExpressionMammalianMutationPromoterCMVAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG13 (450-517)
Plasmid#203558PurposeMammalian expression of GST tagged ATG13 (450-517)DepositorInsertATG13 (ATG13 Human)
UseTagsGSTExpressionMammalianMutationPromoterCMVAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TSF-ATG13 (363-517)
Plasmid#203550PurposeMammalian expression of TwinStrep-Flag tagged ATG13 (363-517)DepositorInsertATG13 (ATG13 Human)
UseTagsTwinStrep FlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG13 (6-196)
Plasmid#203559PurposeMammalian expression of GST tagged ATG13 (6-196)DepositorInsertATG13 (ATG13 Human)
UseTagsGSTExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG13 (363-398)
Plasmid#203554PurposeMammalian expression of GST tagged ATG13 (363-398)DepositorInsertATG13 (ATG13 Human)
UseTagsGSTExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG13 (402-450)
Plasmid#203557PurposeMammalian expression of GST tagged ATG13 (402-450)DepositorInsertATG13 (ATG13 Human)
UseTagsGSTExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 AMSHLP 16-218
Plasmid#180609PurposeBacterial expression for AMSHLP MIT domain residues 16-218. Internal ID: WISP20-83DepositorInsertAMSHLP residues 16-218
UseTagsHIS-SUMOExpressionBacterialMutationResidues 16-218PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG13 (363-517)
Plasmid#203551PurposeMammalian expression of GST tagged ATG13 (363-517)DepositorInsertATG13 (ATG13 Human)
UseTagsGSTExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG13 (363-392)
Plasmid#203555PurposeMammalian expression of GST tagged ATG13 (363-392)DepositorInsertATG13 (ATG13 Human)
UseTagsGSTExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG13 (402-517)
Plasmid#203556PurposeMammalian expression of GST tagged ATG13 (402-517)DepositorInsertATG13 (ATG13 Human)
UseTagsGSTExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-msfGFP-ATG13 (1-197)
Plasmid#203560PurposeMammalian expression of GFP tagged ATG13 (1-197)DepositorInsertATG13 (ATG13 Human)
UseTagsmsfGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
H5_fold-0_Elsa
Plasmid#201825PurposeExpresses H5_fold-0_Elsa in bacterial cellsDepositorInsertH5_fold-0_Elsa
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXC3cIfm
Plasmid#193750PurposeExpresses frame-shifted CI under the control of PLUX promoter, p15A origin of replication, Chloramphenicol selectionDepositorInsertFrame-shifted CI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLUXAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOBD2
Plasmid#196826PurposeYeast two-hybrid ; Gal4 DNA binding domainDepositorTypeEmpty backboneUseTagsGal4 DNA binding domainExpressionYeastMutationPromoterADHAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only