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dora[A]_rescue_construct

Plasmid

#190639

Purpose

Puromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Use

Tags

Expression

Insect

Mutation

Truncated to contain amino acids 1-945

Promoter

Actin-5c

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0033

Plasmid

#185624

Purpose

Level 0 promoter from N. benthamiana used for sgRNA expression, nonstandard overlaps, GGAG-CTCG

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

NbU6-2

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0032

Plasmid

#185623

Purpose

Level 0 promoter from N. benthamiana used for sgRNA expression, nonstandard overlaps, GGAG-TTCG

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

NbU6-1

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pJK434

Plasmid

#155378

Purpose

PphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas9 (bacteria)
PA4-mVenus
PphlF_A4NT in dCas9 sgRNA oscillator v1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Promoter

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pJK422

Plasmid

#155373

Purpose

dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 412; pOSIP)

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas9 (bacteria)
PA4-mVenus
dCas9 sgRNA oscillator v1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Promoter

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pJK423

Plasmid

#155374

Purpose

dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 413; pOSIP)

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas9 (bacteria)
PA4-mVenus
dCas9 sgRNA oscillator v1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Promoter

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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PX458-Lck

Plasmid

#159430

Purpose

CRISPR knockout of Lck

Depositor

Arthur Salomon

Article

Chua et al J Proteome Res. 2020 Nov 13. doi: 10.102

Insert

sgRNA targeting Lck exon 2 (LCK Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pKM328

Plasmid

#134243

Purpose

Mammalian expression construct expressing sgRNA against PLK1

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

gRNA targeting PLK1 3'

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pFA0057

Plasmid

#131784

Purpose

Guide RNA (gGFP) and Cas9 expression plasmid for cleaving pFA6 series GFP C-terminal tagging cassettes, including GFP-His3MX6. Used to perform CRISPR-Swap of alleles.

Depositor

Frank Albert

Article

Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

Insert

20mer GFP cassette guide (gGFP) and 5' sgRNA

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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PLK4 G8.1 gRNA

Plasmid

#90837

Purpose

3rd generation lentiviral gRNA plasmid targeting human PLK4

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PLK4 (Guide Designation G8.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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PLK4 G7.1 gRNA

Plasmid

#90836

Purpose

3rd generation lentiviral gRNA plasmid targeting human PLK4

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PLK4 (Guide Designation G7.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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MIS18BP1 F10.1 gRNA

Plasmid

#90769

Purpose

3rd generation lentiviral gRNA plasmid targeting human MIS18BP1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MIS18BP1 (Guide Designation F10.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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MCM6 E1.2 gRNA

Plasmid

#90762

Purpose

3rd generation lentiviral gRNA plasmid targeting human MCM6

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MCM6 (Guide Designation E1.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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MASTL F4.1 gRNA

Plasmid

#90753

Purpose

3rd generation lentiviral gRNA plasmid targeting human MASTL

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MASTL (Guide Designation F4.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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DYNLT3 B3.5 gRNA

Plasmid

#90670

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYNLT3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DYNLT3 (Guide Designation B3.5)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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DYNLRB1 H11.3 gRNA

Plasmid

#90664

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYNLRB1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DYNLRB1 (Guide Designation H11.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pCR1300

Plasmid

#111119

Purpose

Expresses USP1_+_62903144.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

USP1 gRNA (USP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1324

Plasmid

#111120

Purpose

Expresses WDR48_+_39093886.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

WDR48 gRNA (WDR48 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1278

Plasmid

#111121

Purpose

Expresses XRCC2_-_152373174.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

XRCC2 gRNA (XRCC2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1279

Plasmid

#111122

Purpose

Expresses XRCC2_+_152373143.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

XRCC2 gRNA (XRCC2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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