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Plasmid#153234PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only
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pcDNA3.1(+)-sapphireAKAR-NLS
Plasmid#118467Purposecp-T-sapphire-based single-color PKA biosensor targeted to the nucleus.DepositorInsertsapphireAKAR-NLS
UseTagsNuclear localization signal (NLS)ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU2-A-RFP (colE1 origin)
Plasmid#74093PurposepTU2-A backbone, with RFP negative selection and colE1 (medium-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 4 TU's with RFP and colE1 origin of replication
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterR0010 Const. Promoter controlling RFPAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/GUG-nLuc-3XFLAG
Plasmid#127301PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHygroR-GMRWhite
Plasmid#165886PurposeHygromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screeningDepositorInsertTransgenesis Ready Selection Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV/3' Box_(GLuc)_INT
Plasmid#68436PurposeTransient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.DepositorInsertINT construct_bearing three PP7 Stem-loops
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pzyxin-emiRFP703
Plasmid#136568PurposeZyxin labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)DepositorInsertzyxin-emiRFP703
UseTagsemiRFP703ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGM65881
Plasmid#153215PurposeLevel 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformationDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131A2.0
Plasmid#99884PurposeGolden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133C2.0
Plasmid#99893PurposeGolden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU6Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cyan Nano-lantern/pcDNA3
Plasmid#67660Purposecyan luminescent protein for high-speed single-cell and whole body imagingDepositorInsertCyan Nano-lantern
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGE651
Plasmid#153229PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131C2.0
Plasmid#99886PurposeGolden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU6Available sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132C2.0
Plasmid#99889PurposeGolden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU6Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
Orange Nano-lantern/pcDNA3
Plasmid#67661Purposeorange luminescent protein for high-speed single-cell and whole body imagingDepositorInsertOrange Nano-lantern
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
BPK1169: CAG-DmrC-NLS-3xFLAG-P65
Plasmid#104564PurposeMammalian expression vector for DmrC fused to p65 activation domainDepositorInsertDmrC-p65
UseTags3x FLAG and NLSExpressionMammalianMutationPromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS423-FjTAL(JBEI-15466)
Plasmid#87936PurposeExpression in S.cerevisiae of FjTALDepositorInsertFjTAL
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
YFP-FKBPx5
Plasmid#103775Purposeexpresses iPOLYMER component in mammalian cellsDepositorInsertYFP-FKBPx5
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral CaMKKbeta
Plasmid#33322DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
UseLentiviralTagsFlagExpressionMutationPromoterhuman PGKAvailable sinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ExRai-AKAR(T/A)
Plasmid#118406PurposeNegative-control mutant for ExRai-AKAR biosensor.DepositorInsertExRai-AKAR(T/A)
UseTags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable sinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only