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Plasmid#136254PurposeDoxycycline inducible piggyBAC plasmid for mammalian expression of cytosine base editor AncBE4maxDepositorInsertAncBE4max_GFP
UseCRISPR; Piggybac dna transposonTagsExpressionMammalianMutationThe AncBE4max_GFP insert was PCR amplified from a…PromoterTRE3GAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet3-Giantin_C1
Plasmid#189773PurposeConstruct for labelling Golgi apparatus in mammalian cells using mScarlet3-Giantin fusionDepositorInsertmScarlet3-Giantin
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-mKate2.5f
Plasmid#99129PurposeAlso known as pAAV-GFAP-mKate2.5-f. An AAV genome that express the farnesylated fluorescent protein mKate2.5 from the GFAP promoterDepositorInsertmKate2.5f
UseAAVTagsExpressionMammalianMutationPromoterGFAPAvailable sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti MS2-P65-HSF1_Hygro
Plasmid#61426Purposelenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2DepositorInsertMS2-P65-HSF1_2A_Hygro (HSF1 Human, Synthetic)
UseCRISPR and LentiviralTagsExpressionMammalianMutationN55K in MS2PromoterEF1AAvailable sinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-dnMCAK
Plasmid#208650PurposeMammalian expression plasmid expressing eGFP-dnMCAK driven by CMV promoterDepositorInsertKif2c (Kif2c Cricetulus griseus (Chinese hamster))
UseTagseGFPExpressionMammalianMutationS92A , S106A , S108A, S112A , S186A, H530A, R534A…PromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH-ePPE
Plasmid#183097PurposeFor plant prime editing in rice plantsDepositorInsertNls-nCas9-NC-nls-MLV-Nls
UseCRISPRTagsExpressionPlantMutationD10A in SpCas9PromoterAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYLsgRNA-AtU6-1
Plasmid#66202Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLAG-PRMT3
Plasmid#164695PurposeExpression of PRMT3 with an N-terminal FLAG tagDepositorInsertPRMT3 (PRMT3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Mammalian_SpCas9_Target-AID3S
Plasmid#188649PurposeExpresses SpCas9_Target-AID3S in mammalian cellsDepositorInsertnCas9-AID3S-UGI
UseTagsUGIExpressionMammalianMutationD10A for SpCas9PromoterpCMVAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF3058
Plasmid#144534PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertEBF1 (EBF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only