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pIK137
Plasmid#65632PurposeExpresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter and contains a C. briggsae unc-119 rescuing fragmentDepositorInsertPeft-3::gfp::h2b::tbb-2 3'UTR, C. briggsae unc-119 rescue fragment
UseTagsExpressionWormMutationPromoterAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF Doc2alpha D291N mutant GFP
Plasmid#128821PurposeTo visualize Doc2alpha D291N mutant in PC12 cellsDepositorInsertDoc2alpha (Doc2a Rat)
UseTagsEGFPExpressionMammalianMutationD291NPromoterEF-1alphaAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF Doc2beta D303N mutant GFP
Plasmid#128822PurposeTo visualize Doc2beta D303N mutant in PC12 cellsDepositorInsertDoc2beta (Doc2b Rat)
UseTagsEGFPExpressionMammalianMutationD303NPromoterEF-1alphaAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-Grb10-WT
Plasmid#74501PurposeOverexpression of Grb10 in mammalian cellsDepositorInsertGrb10 (GRB10 Human)
UseTagsFLAGExpressionMammalianMutationL358S, V522A, and V524LPromoterCMVAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Sa sgRNA expression vector
Plasmid#107720PurposeFor in vitro transcription of Sa sgRNA from the T7 promoter.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab7 DN
Plasmid#44826DepositorInsertRab7 (Rab7 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationT22N (dominant negative)PromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-Alpha-Actinin-19
Plasmid#57944PurposeLocalization: Alpha Actinin, Excitation: 549, Emission: 565DepositorInsertAlpha-Actinin (ACTN1 Human)
UseTagsmOrange2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
kank3_pJet1.2
Plasmid#121981Purposefor generating kank3 riboprobes for WISH in zebrafishDepositorInsertkank3 (kank3 Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterT7Available sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMiNGo11-3
Plasmid#126058PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cellDepositorInsertsmitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11x3 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMiNGo11-7
Plasmid#126057PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cellDepositorInsertsmitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11x7 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab7 CA
Plasmid#37704DepositorInsertRab7 (Rab7 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ67L (constitutive active)PromoterGAL4Available sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab8 CA
Plasmid#37707DepositorInsertRab8 (Rab8 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ67L (constitutive active)PromoterGAL4Available sinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab8 DN
Plasmid#37706DepositorInsertRab8 (Rab8 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationT22N (dominant negative)PromoterGAL4Available sinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab6 CA
Plasmid#37698DepositorInsertRab6 (Rab6 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ71L (constitutive active)PromoterGAL4Available sinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab6 DN
Plasmid#37697DepositorInsertRab6 (Rab6 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationT26N (dominant negative)PromoterGAL4Available sinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mEos4a (mEosFP4a-V69T)
Plasmid#99225PurposeBacterial expression of the primed conversion capable protein pr-mEos4a (mEos4a-V69T).DepositorInsertpr-mEos4a
UseTagsExpressionBacterialMutationV69T for primed conversion applicationsPromoterT7Available sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-Maf
Plasmid#98179PurposeExpression of a cMaf in situ hybridization probeDepositorInsertcMaf, partial mRNA (Maf Mouse)
UseIn situ hybridizationTagsExpressionMutationPromoterT3/T7Available sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-rap1-K462-463-651R
Plasmid#84564PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorInsertRAP1 (RAP1 Budding Yeast)
UseGateway donor plasmidTagsExpressionMutationK462-463-651RPromoternoneAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-rap1-K43-117-118R
Plasmid#84565PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorInsertRAP1 (RAP1 Budding Yeast)
UseGateway donor plasmidTagsExpressionMutationK43-117-118RPromoternoneAvailable sinceOct. 19, 2016AvailabilityAcademic Institutions and Nonprofits only
