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Plasmid#169996PurposeUltra processive kinesin KIF1A fused with KIF5B stalk for dimerization and HALO-tag for labelingDepositorInsertKinesin-like protein Unc-104 fused with KIF5B
UseTagsHALO-tag, 6xHISExpressionBacterialMutationPromoterT7Available sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
ZJOM10
Plasmid#133639PurposeIntegration of VPS4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast late endosome.DepositorInsertp1k-Vps4-GFP (VPS4 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
SiRES COR1C-GFP
Plasmid#195154Purposeimage COR1CDepositorInsertCORO1C (CORO1C Human)
UseTagsExpressionMammalianMutationmutations to make si RNA resistantPromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV eIF4E IRES GFP
Plasmid#18761DepositorInserteIF4E (Eif4e Mouse)
UseRetroviralTagsIRES GFPExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
ZJOM86
Plasmid#133634PurposeIntegration of ANP1-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast early golgi.DepositorInsertp1k-ANP1-GFP (ANP1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ER-CaNAR2
Plasmid#64732PurposeEndoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of ER membrane.DepositorInsertCYP450-CaNAR2
UseTagsCerulean3, N-terminal 27 amino acids from CYP450,…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_21A
Plasmid#91128PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:hpt IIDepositorInsertEngineering Reagent: 35S:AtCas9 + AtU6:gRNA
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 mSynCAM 2
Plasmid#115497Purposeexpression of mouse full-length SynCAM 2DepositorInsertCadm2 (Cadm2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRRL-5xTetO-pEF-H2B-Citrine-SV40
Plasmid#186966PurposeLentiviral vector encoding fluorescent reporter (5xTetO-pEF-H2B-Citrine-SV40) for expression in mammalian cells.DepositorInsert5xTetO-pEF-H2B-Citrine-SV40
UseLentiviralTagsExpressionMammalianMutationPromoter5xTetO-pEF-1alphaAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 Intergenic control guide 2
Plasmid#193585PurposesgRNA control; induces CAS9 cutting in an intergenic regionDepositorInsertsgRNA control
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgNFkB2 guide 2
Plasmid#193594PurposeNFkB2 knockoutDepositorInsertsgNFkB2 guide 2 (NFKB2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-p3-PE(1152)-N-term
Plasmid#187181Purposep3-PE2-N-term(1152) in AAV2 backbone (ITRs)DepositorInsertPE2 from plasmid #132775
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEM390 - mosTI unc-119 - Peft-3 - NNN - tbb-2
Plasmid#182350PurposeUbiquitous expression cloning vector for mosTI(unc-119). Transgene inserted between Peft-3 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAXEF-Rap1-E63(GTP)
Plasmid#32698DepositorInsertRAP1 (RAP1A Human)
UseTagsExpressionMammalianMutationE63: Consitutively GTP-boundPromoterEF promoterAvailable sinceDec. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hyg-RFPKDEL
Plasmid#138660PurposeReporter plasmid expressing the ER-located RFPDepositorInsertRFP-KDEL
UseTagsKDELExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE-SHH
Plasmid#134350PurposeTargeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locusDepositorInsertSHH (SHH Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5 TGF beta type I receptor Flag
Plasmid#14831DepositorInsertTGF beta type I receptor (Tgfbr1 Rat)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
ZJOM25
Plasmid#133672PurposeIntegration of MSP1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on mitochondria and peroxisomes.DepositorInsertp1k-MSP1-2GFP (MSP1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-1
Plasmid#172767PurposeBacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-1
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_YWHAZ_14-3-3
Plasmid#109808PurposeProtein expression and purification of YWHAZ_14-3-3DepositorInsertYWHAZ_14-3-3 (YWHAZ Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only