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Plasmid#118463PurposeNegative-control mutant for ExRai-AktAR biosensor.DepositorInsertExRai-AktAR(T/A)
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUSE-Src-YF-UniRapR-mCerulean-myc
Plasmid#45381DepositorInsertSrc-YF-UniRapR-mCerulean-myc
UseTagsMyc, Myr, and mCeruleanExpressionMammalianMutationchanged mSrc Tyrosine 529 to PhenylalaninePromoterCMVAvailable sinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) 6xHis-TEV-IFIT1
Plasmid#53557Purposebacterial expression of IFIT1DepositorInsertIFIT1 (IFIT1 Human)
UseTags6xHIS and TEVExpressionBacterialMutationPromoterT7Available sinceMay 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-hAAT-W
Plasmid#126704PurposeConstituitive, ubiquitious human alpha - 1 antitrypsin gene expressionDepositorInserthAAT (SERPINA1 Human)
UseLentiviralTagsExpressionMutationPromoterCMVpAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pALS099 (pGEX-2TP-cTevHis-DEST)
Plasmid#139787PurposeGateway destination vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHisDepositorTypeEmpty backboneUseTags6xHis (cleavable by TEV protease) and GST (cleava…ExpressionBacterialMutationPromoterAvailable sinceApril 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEM394 - mosTI unc-119 - PUPN - NNN - tbb-2
Plasmid#182352PurposePan-neuronal expression cloning vector for mosTI(unc-119). Transgene inserted between Ultra pan neuronal (UPN) promoter and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
ZJOM100
Plasmid#133662PurposeIntegration of MNN9-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.DepositorInsertp1k-MNN9-mTagBFP2 (MNN9 Budding Yeast)
UseTagsmTagBFP2ExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC-85b
Plasmid#53282PurposeContains crtE, crtI, crtY genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and will produce beta-carotene in E. coli when complemented with a gene encoding phytoene synthaseDepositorInsertcrtE, crtY, crtI
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDB180
Plasmid#80677Purposefor secretion of N-terminal OsmY, deca-histidine, SUMO tagged proteinDepositorTypeEmpty backboneUseTagsOsmY-10His-Smt3ExpressionBacterialMutationPromoterT7Available sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
ZJOM157
Plasmid#133640PurposeIntegration of GFP-PEP12 as an additional copy in genome, use auxotrophic marker URA3(Kluyveromyces lactis).Marker for yeast late endosome.DepositorInsertp1k-GFP-Pep12 (PEP12 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS3-lim-4P_gtACR2_SL2_eGFP(65C)_unc-54 3'UTR
Plasmid#195853PurposeExpresses inhibitory opsin gtACR2 and GFP in turning associated neuronsDepositorInsertGuillardia theta anion-conducting channelrhodopsin-2 (GtACR2)
UseTagsExpressionWormMutationPromoterlim-4Available sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
ZJOM1
Plasmid#133632PurposeIntegration of GFP-SED5 as an additional copy in genome, use auxotrophic marker URA3(Kluyveromyces lactis). Marker for yeast early golgi.DepositorInsertp1k-GFP-SED5 (SED5 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
LZF97_hSyn-spine-jRGECO1a
Plasmid#119198PurposeTargets jRGECO1a to spines in living neurons.DepositorInsertjRGECO1a-TPR3-Utrophin(1-78)-TPR3-Zfn(CCR5-L) (Utrn Rat, Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterHuman Synapsin 1Available sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
ZJOM57
Plasmid#133656PurposeIntegration of PEX3-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast peroxisome.DepositorInsertp1k-PEX3-DuDre (PEX3 Budding Yeast)
UseTagsDuDreExpressionYeastMutationPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RAB-EKARev(T/A)
Plasmid#118475PurposeNegative-control mutant for RAB-EKARev biosensor.DepositorInsertRAB-EKARev(T/A)
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
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rAAV-syn::FLEX-rev::PSAML141F,Y115F:5HT3HC-IRES-GFP
Plasmid#32477DepositorInsertPSAML141F,Y115F-5HT3HC
UseAAV and Cre/Lox; Adeno-associated virus, flex swi…TagsIRES-EGFPExpressionMutationPromoterSynapsinAvailable sinceJan. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2
Plasmid#87694PurposeExpress ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cellsDepositorInsertERT2-PhoCl-Cre-PhoCl-ERT2
UseTagsExpressionMammalianMutationPromoterChicken beta actinAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
IRES-GFP-AXL-KD
Plasmid#65498PurposeExpresses kinase dead AXL in mammalian cells with cistronic EGFPDepositorInsertTyrosine-protein kinase receptor UFO (AXL Human)
UseTagsFLAGExpressionMammalianMutationK567RPromoterCMVAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only