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pCMV_msfGFP-SEPT7 Gmut1

Plasmid

#180316

Purpose

mammalian expression of human SEPT7 Gmut1 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT7 W269A, H279D

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-mApple-CAAX

Plasmid

#180324

Purpose

mammalian expression of human SEPT9_i3 fused to monomeric Apple and CAAX (HRas)

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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CXCR4-SZ158a-sfGFP

Plasmid

#162446

Purpose

Expression in HEK293T cell and compete ligand singaling against full-length receptors

Depositor

Shuguang Zhang

Article

Qing et al iScience. 2020 Oct 28;23(12):101670. doi

Insert

C-X-C chemokine receptor type 4 (CXCR4 Human)

Use

Tags

sfGFP

Expression

Mammalian

Mutation

truncation from aa52 to aa257

Promoter

CMV

Available since

Availability

Academic Institutions and Nonprofits only

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pEM01

Plasmid

#135414

Purpose

Expression of Hygromycin resistance from CMV promoter

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

CMVpr hph ADH1t

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

CMVpr

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pUCM-CLYBL-hNIL

Plasmid

#105841

Purpose

Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron

Depositor

Michael Ward

Article

Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

Inserts

Neurogenin 2 (NEUROG2 Human)
ISL LIM Homeobox 1 (ISL1 Human)
LIM Homeobox 3 (LHX3 Human)
mCherry
rtTA3G

Use

CRISPR and TALEN

Tags

Expression

Mutation

Promoter

CAG, EF-1alpha, and TRE3G

Available since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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PB-tetO-hNIL

Plasmid

#197089

Purpose

PiggyBac plasmid with tet-inducible expression of transcription factors for motor neuron differentiation

Depositor

Brian Wainger

Article

Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

Insert

NGN2-P2A-ISL1-T2A-LHX3 (NEUROG2, ISL1, LHX3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCjedCas9

Plasmid

#172216

Purpose

Expresses catalytically inactive Campylobacter jejuni Cas9 (CjedCas9) in bacterial cells

Depositor

John van der Oost

Article

Saha et al Sci Adv. 2020 Jun 17;6(25):eaaz4849. doi

Insert

catalytic mutant of Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system

Use

Tags

Histag

Expression

Mutation

changed Asp 8 to Ala and His 559 to Ala

Promoter

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI-PGK-HYGRO-GATA6mut-HA

Plasmid

#65476

Purpose

Lentiviral expression of GATA6 Znc-finger mutant

Depositor

Eduard Batlle

Article

Whissell et al Nat Cell Biol. 2014 Jul;16(7):695-707. d

Insert

GATA6mut (GATA6 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

lacking the eight most highly conserved bases of …

Promoter

PGK

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pEF2-FL-hMer_gR1

Plasmid

#225993

Purpose

Expresses a chimeric contruct having N-terminal human MerTK and C-terminal human Interferon Gamma Receptor

Depositor

Sergei Kotenko, Raymond Birge

Article

Tsou et al J Biol Chem. 2014 Sep 12;289(37):25750-6

Insert

human MerTK N-Terminal human Interferon Gamma R1 Receptor C-Terminal fusion (MERTK, IFNGR1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1-a

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEF2-EFGR-hAxl

Plasmid

#225997

Purpose

Expresses a chimeric contruct having N-terminal human EGFR and C-terminal human Axl

Depositor

Sergei Kotenko, Raymond Birge

Article

Kimani et al Cell Commun Signal. 2016 Sep 6;14(1):19.

Insert

human EGFR N-Terminal human Axl C-Terminal Fusion (EGFR, AXL Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1-a

Available since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3_N-DYK_MTHFD1-NLS

Plasmid

#133023

Purpose

NLS sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)

Depositor

Stefan Kubicek

Article

Sdelci et al Nat Genet. 2019 Jun;51(6):990-998. doi:

Insert

MTHFD1 (MTHFD1 Human)

Use

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3_N-DYK_MTHFD1-NES

Plasmid

#133022

Purpose

NES sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)

Depositor

Stefan Kubicek

Article

Sdelci et al Nat Genet. 2019 Jun;51(6):990-998. doi:

Insert

MTHFD1 (MTHFD1 Human)

Use

Tags

NES

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-beta2-EGFP

Plasmid

#160979

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform beta2 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta2 (MOG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM4673

Plasmid

#48014

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pAGM1311

Plasmid

#47983

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pICH41308

Plasmid

#47998

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGMV-U

Plasmid

#112797

Purpose

Universal BeYDV-derived vector for cloning of up to four gRNAs

Depositor

Humberto Prieto

Article

Prieto et al In: Cantu D., Walker M. (eds) The Grape

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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p15aTetT7mut-fuseYFP

Plasmid

#28013

Depositor

Lingchong You

Article

Tan et al Nat Chem Biol. 2009 Nov . 5(11):842-8.

Insert

EYFP-fuse, T7 RNAP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 21, 2011

Availability

Academic Institutions and Nonprofits only

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pICH41295

Plasmid

#47997

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pT7oGFP

Plasmid

#69346

Purpose

Orthogonal GFP message under T7 promoter

Depositor

Michael Jewett

Article

Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

Insert

Super folder GFP

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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